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OfflineMoxyOx
Grazin'

Registered: 10/08/10
Posts: 1,439
Loc: Flag
Last seen: 1 month, 20 days
Re: Flow hood alternatives? [Re: enlightened seed]
    #22363260 - 10/11/15 01:11 PM (8 years, 3 months ago)

It would. Essentially it is the same as a glovebox, but it does have a larger opening than two circles.

How do you clean the device?


--------------------
No one behind, no one ahead.
The path the ancients cleared has closed.
And the other path, everyone's path,
easy and wide, goes nowhere.
I am alone and find my way.


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OfflineRJ Tubs 202
Male


Registered: 09/20/08
Posts: 6,016
Loc: USA Flag
Last seen: 9 hours, 53 minutes
Re: Flow hood alternatives? [Re: Psilosopherr]
    #22376364 - 10/13/15 11:00 PM (8 years, 3 months ago)

Quote:

rbalzer said:
iso alcohol is a poor substitute for flame sterilization. most people on here will tell you that.

I just keep a propane torch lit on a table near my SAB. whenever I need to flame I simply pull said item out of the SAB and apply the flame.

Just gotta keep safety in mind.




I'm used to using a flow hood, but recently I've been using a glove box, and once I start working, I don't want to pull out (insert dirty joke here) . . .

(paranoia is a factor)

But seriously, thanks for that comment. I needed that. It's tough to change one's habit . . .


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Invisibleenlightened seed
Utopia is a state of mind
Male User Gallery


Registered: 05/04/07
Posts: 2,117
Loc: amongst civilization
Re: Flow hood alternatives? [Re: RJ Tubs 202]
    #22376384 - 10/13/15 11:06 PM (8 years, 3 months ago)

personally i like a SAB(glove box) with plexiglass on the top instead of the lid that comes with it.


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Invisibleenlightened seed
Utopia is a state of mind
Male User Gallery


Registered: 05/04/07
Posts: 2,117
Loc: amongst civilization
Re: Flow hood alternatives? [Re: RJ Tubs 202]
    #22376508 - 10/13/15 11:38 PM (8 years, 3 months ago)

Quote:

RJ Tubs 202 said:
Quote:

rbalzer said:
iso alcohol is a poor substitute for flame sterilization. most people on here will tell you that.

I just keep a propane torch lit on a table near my SAB. whenever I need to flame I simply pull said item out of the SAB and apply the flame.

Just gotta keep safety in mind.




I'm used to using a flow hood, but recently I've been using a glove box, and once I start working, I don't want to pull out (insert dirty joke here) . . .

(paranoia is a factor)

But seriously, thanks for that comment. I needed that. It's tough to change one's habit . . .




paranoia sucks i used to get paranoid(in certain situations) but now i have no reason to  :pipesmoke:


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Invisiblemicro
bunbun has a gungun
Male User Gallery


Registered: 05/09/03
Posts: 7,532
Loc: Brick City Flag
Re: Flow hood alternatives? [Re: IngSocTHC]
    #22391982 - 10/17/15 06:21 AM (8 years, 3 months ago)

Quote:

IngSocTHC said:
Micro: I was under the impression that useing a flame like that to keep an area clean doesn't work. Any recent discussions you might be able to point me towards?




Google?

Seriously, this is common aseptic technique.

Here is a Youtube video:



Believe Bio Rad if you can trust them.

This is common practice and can be found very easily in a search.

CLICK ME FOR A WHOLE LOT MORE

EDIT: If people keep asking this I'm gonna keep deeplinking threads and googlebomb the shit out of it even more just to sink it into your heads :V

(I'm kidding of course, well not the googlebombing part cause I find it amusing it is there on the first page already but... the facepalming part cause I really don't care)

This was another googlebomb anyway.

EDIT EDIT: Darn, no it wasn't but now it is :lol:


--------------------
Any research paper or book for free
(Avatar is Maxxy, a character by Mizzyam, RIP)


Edited by micro (10/17/15 07:15 AM)


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OfflinePussyFart
Retired Cultivation Extrodinaire
Male


Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 17 days, 21 hours
Trusted Cultivator
Re: Flow hood alternatives? [Re: micro]
    #22403435 - 10/19/15 12:48 PM (8 years, 3 months ago)

Bunsen burners get used in labs where bacteria is grown out and the cultures are tossed within a week.....we keep our cultures for far longer, so we need to take more precautions than that......the next best thing to a LFH is a SAB.....


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Invisiblemicro
bunbun has a gungun
Male User Gallery


Registered: 05/09/03
Posts: 7,532
Loc: Brick City Flag
Re: Flow hood alternatives? [Re: PussyFart]
    #22406782 - 10/20/15 12:01 AM (8 years, 3 months ago)

so wait, i'm lost

is this an appeal to authority argument or appeal to oneself

because that is devoid of any substance whatsoever but

it IS

it IS because I SAY IT IS



appeal to posts that at least have some content :lol:

hoorah (i'm sure you get the joke :V )


--------------------
Any research paper or book for free
(Avatar is Maxxy, a character by Mizzyam, RIP)


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Offlinefruit
Registered: 10/22/15
Posts: 13
Last seen: 8 years, 2 months
Re: Flow hood alternatives? [Re: micro]
    #22418203 - 10/22/15 12:56 PM (8 years, 3 months ago)

So how would one prepare a Sab for use then? Lysol and wait? Alcohol wipedown?


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OfflineMoxyOx
Grazin'

Registered: 10/08/10
Posts: 1,439
Loc: Flag
Last seen: 1 month, 20 days
Re: Flow hood alternatives? [Re: fruit]
    #22418681 - 10/22/15 02:55 PM (8 years, 3 months ago)

Reduce the amount of contaminant in the air and the work area.

So spray the environment down with lysol and wipe the SAB with alcohol.

The most important process is your technique though, fidgeting and moving quickly and hastily will fuck you over more than anything.


--------------------
No one behind, no one ahead.
The path the ancients cleared has closed.
And the other path, everyone's path,
easy and wide, goes nowhere.
I am alone and find my way.


Extras: Filter Print Post Top
Offlinefruit

Registered: 10/22/15
Posts: 13
Last seen: 8 years, 2 months
Re: Flow hood alternatives? [Re: MoxyOx]
    #22418977 - 10/22/15 04:15 PM (8 years, 3 months ago)

thanks. didnt know if there might be any special attention it should get or not


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Invisibledreadhed
 User Gallery
Registered: 01/21/11
Posts: 175
Re: Flow hood alternatives? [Re: fruit]
    #22420172 - 10/22/15 08:36 PM (8 years, 3 months ago)

I use a big plastic tub placed upside down. First I spray the air in the area Im going to be working in with soapy water. I use to use Lysol but didn't get more contamination when I switched to just soapy water. Wipe my SAB down with ISO, put in on a clean towel, spray the inside with soapy water, wipe my jars, tools etc with iso & put them in the box. Some times I'll spay once more with soapy water. Then wait a couple of min then get to work.


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Offlinefruit

Registered: 10/22/15
Posts: 13
Last seen: 8 years, 2 months
Re: Flow hood alternatives? [Re: dreadhed]
    #22431031 - 10/25/15 09:11 AM (8 years, 3 months ago)

is it not difficult to dry the box out when you have sprayed all that stuff? not much airflow would mean little evap. i personally noticed problems evaping alcohol, of all things in my old glovebox/sab


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Invisibledreadhed
 User Gallery
Registered: 01/21/11
Posts: 175
Re: Flow hood alternatives? [Re: fruit]
    #22433579 - 10/25/15 08:07 PM (8 years, 3 months ago)

I don't dry it out. Just a few mists to bring down and spores, etc that may be in the air.  I don't spray it till water is dripping off the top or anything like that.


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