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OfflinefirstTIMER420
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pink oyster grow/questions
    #22335946 - 10/05/15 08:02 AM (8 years, 3 months ago)

Innocukayed some jars with a liquid culture syringe from a vendor, so I assumed it was clean but but I guess I was wrong.

Never grown oysters before so I was wondering if this yellowing was normal? Also doesn't seem like its completely colonizing on all sides, what I mean by that is it isn't as thick on some sides.



I'm gonna spawn these in some buckets to pasteurized straw, seems like the easiest avenue to go.

Also, if anyone has some suggestions on pasteurizing straw please share!


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Re: pink oyster grow/questions [Re: firstTIMER420]
    #22336003 - 10/05/15 08:22 AM (8 years, 3 months ago)

just keep them warm during colonization.  make sure they get some air to breathe.  if you're doing straw buckets, and you use more than a couple quarts of spawn...you could see pins in less than two weeks!  Even one!  Pinks are amazingly fast which is why you can cold pasteurize your straw or not even treat it at all!  Just soak and inoculate.  Usually for me they are colonized in a week or less and pinning on day 10-14.


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Re: pink oyster grow/questions [Re: drake89]
    #22336583 - 10/05/15 11:45 AM (8 years, 3 months ago)

I had pinks on straw pinning 7 days after spawning at 10%. They were. Pinning in my pantry where I colonize. I heat pasteurized using a bath around 160-165 to start and put the pre soaked straw with gypsum into 5 gal paint strainer bags but pillow cases or even better burlap bags work too. 90 min at 136-150 drain and lay out on a table to cool with a fan turning every half hour. 1-2 hours later its cooled enough to spawn.


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Re: pink oyster grow/questions [Re: Gr0wer]
    #22336588 - 10/05/15 11:48 AM (8 years, 3 months ago)

Soak your straw for 1-2 days. Use a dash of dish soap to help the straw absorb more water.


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OfflinefirstTIMER420
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Re: pink oyster grow/questions [Re: drake89]
    #22336758 - 10/05/15 12:27 PM (8 years, 3 months ago)

I was wondering how wet the straw should be after soaking. If its dripping I'm assuming its too wet.

Those jars look OK to you? I'm worried about the yellow on top.


Also, I was going to use 2 QTS spawn per 5gal bucket but what do you guys recommend?

And I've been reading about misting them after the fruits start growing, what's your opinion on that?


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Edited by firstTIMER420 (10/05/15 12:29 PM)


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Re: pink oyster grow/questions [Re: firstTIMER420]
    #22336788 - 10/05/15 12:38 PM (8 years, 3 months ago)

Let the straw drip dry, using the fan to dry and cool helps remove excess moisture as well. I drain and cool my strawon a slanted table so the water drains away. Mist for sure. Two jars per 5 gal pail works for me. I've been having much better results with pinks in bags vs pails. The yellow water is myc piss. I'm supprised those arnet pinning already invetro. My pinks start to pin on agar and seed a few days after colonization. You might want to fruit a jar to verify the culture.


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Re: pink oyster grow/questions [Re: Gr0wer]
    #22343375 - 10/06/15 10:57 PM (8 years, 3 months ago)

my jars started turning pink so I shook them, should I not have done that?

I didn't have time to make straw yesterday, so im doing it tomorrow. 

I think ill try bags next time, whats your procedure for them?

last thing, pinning invitro would work? I thought they needed to be mixed with straw to fruit.


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Re: pink oyster grow/questions [Re: firstTIMER420]
    #22343436 - 10/06/15 11:09 PM (8 years, 3 months ago)

They start to grow pink as they finish colonizing, this shows even on agar. You should have left them and just held out for the spawn day. Even if you have a few pins going its still good to use, the pins are just another inoculation point.  A good pink strain will always pin invitro but they need more FAE for a good fruitbody. If your seeing pink myc i think its safe to say you have verified the strain and fruiting a jar wouldn't be needed.


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Re: pink oyster grow/questions [Re: Gr0wer]
    #22343450 - 10/06/15 11:14 PM (8 years, 3 months ago)

gotcha, thanks for the info.

Either way, since I shook, using them for spawn will still be viable, and a good option?  Don't want to just contam.


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Re: pink oyster grow/questions [Re: firstTIMER420]
    #22345007 - 10/07/15 11:59 AM (8 years, 3 months ago)

Yup, your spawn will still be viable and will produce fine.  I just harvested 3 lbs. off of a 22 lbs. log an hour ago and had to do the same thing.  Pinks do everything by their own time table.  I generally have to shake mine to prevent invitro pinning.


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Re: pink oyster grow/questions [Re: shroombasa]
    #22347075 - 10/07/15 07:21 PM (8 years, 3 months ago)

sweet.

how long do you pasteurize for?  Ive been told a couple times ranging from 60 - 120 minutes..

also, it seems like a pain in the ass, why cant I just load with water into jars and heat bath it, then drain the straw for a while and then use.


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Re: pink oyster grow/questions [Re: firstTIMER420]
    #22349798 - 10/08/15 11:50 AM (8 years, 3 months ago)

Quote:

firstTIMER420 said:
sweet.

how long do you pasteurize for?  Ive been told a couple times ranging from 60 - 120 minutes..

also, it seems like a pain in the ass, why cant I just load with water into jars and heat bath it, then drain the straw for a while and then use.



I judge my pasteurization times on the initial bath temperature.  The range I use is 60-90 minutes at 140-160F.  If my starting temp is 160F, I'll soak for 60 minutes, 150F for 75 minutes, and 140F for 90 minutes (although you'll probably need to top up the bath if you're starting that low). 

As far as loading into jars, I'm not sure what you had in mind, but I imagine it'd be difficult to get enough pasteurized straw in your jars to be worth it.  If you're only planning on stuffing a couple of small bags (like a gallon or less), then that might work.  Otherwise, I'd look at RR's videos and try to do something similar with a plastic tub.


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Re: pink oyster grow/questions [Re: shroombasa]
    #22350526 - 10/08/15 03:21 PM (8 years, 3 months ago)

yea im doing that with a plastic tub. but im wondering, do I rinse the straw off after soaking/pasteurizing because of the soap used? Im thinking the soap will mess with the spawn


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Re: pink oyster grow/questions [Re: firstTIMER420]
    #22350761 - 10/08/15 04:21 PM (8 years, 3 months ago)

You don't have to rinse.  Some of the guys on here that use straw baskets pre-soak with soapy water and just heat up the same water for the pasteurization.


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Re: pink oyster grow/questions [Re: shroombasa]
    #22351740 - 10/08/15 07:56 PM (8 years, 3 months ago)

I soapy water soak my straw then heat up a fresh drum of water for the pasteurization, that rinses the soap off enough to where its not noticable. Don't forget to add your gypsum.


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Re: pink oyster grow/questions [Re: Gr0wer]
    #22352521 - 10/08/15 11:27 PM (8 years, 3 months ago)

I forgot the gypsum, shit. But I'm wondering how long straw pasteurized lasts for. I'm tired as fuck and would loile to just spawn to it in the morning


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Re: pink oyster grow/questions [Re: firstTIMER420]
    #22352621 - 10/08/15 11:48 PM (8 years, 3 months ago)

should be fine

I treat my pasteurized substrate like i do my homebrew. Chill fast and give contams less time to get a foothold. If you have a clean environment to chill your substrate i wouldn't sweat it. I chill outdoors where i know there are bugs so i cool fast and spawn within ~2 hours.


Edited by Gr0wer (10/08/15 11:50 PM)


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Re: pink oyster grow/questions [Re: Gr0wer]
    #22372255 - 10/13/15 09:03 AM (8 years, 3 months ago)

I ended up doing two buckets, but for some reason, one is colonizing the other....not so much. I think its been maybe 4 or 5 days and one is almost completely colonized the homes, the other I cannot see really any signs. Should I toss the uncolonized one before it contains(if it already hasnt) ( might have because I used the straw that sunk to the bottom of the Pasteur bath, I needed it.)


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Re: pink oyster grow/questions [Re: firstTIMER420]
    #22372266 - 10/13/15 09:06 AM (8 years, 3 months ago)

smell it. If you do toss it toss it outside in a shady spot and cover with leaves or mulch.


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Re: pink oyster grow/questions [Re: Gr0wer]
    #22379525 - 10/14/15 04:20 PM (8 years, 3 months ago)

Gotcha on that. Heard someone say pink oyster smells, and damn they were right. I hope the fruits fon taste as bad as they smell.

Any suggestions for after pins form? I have them in separate trash bags, tied closely as to allow ge and the lids to the buckets cracked


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Re: pink oyster grow/questions [Re: firstTIMER420]
    #22379917 - 10/14/15 05:48 PM (8 years, 3 months ago)

They shouldn't smell bad. Sounds like you may have a bacterial infection. You can still put them outside and they may do ok.


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Re: pink oyster grow/questions [Re: Jim Morrison]
    #22381113 - 10/14/15 10:01 PM (8 years, 3 months ago)

Well im saying it taste bad because it doesn't smell like cube Myc, or is it supposed too? I've heard multiple people say pinks don't smell very good

EDIT:

I didn't even think about setting up lights for them.. Im assuming they run on a 12:12 light schedule right?


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Edited by firstTIMER420 (10/16/15 10:26 AM)


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Re: pink oyster grow/questions [Re: firstTIMER420]
    #22388228 - 10/16/15 12:49 PM (8 years, 3 months ago)

Pink oysters shouldn't smell bad.  As far as taste, they're more bland than other oysters, but they don't really taste bad either.  It sounds like you've got a contam.

You should definitely set up a lighting schedule.  12/12 will work.


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Re: pink oyster grow/questions [Re: shroombasa]
    #22400668 - 10/18/15 10:51 PM (8 years, 3 months ago)

I thought they were contamed from the yellow stuff on the tops of the jars, but it did look like metabolites I guess. Never grown from lc so maybe that's why

Should I take the, out of the trash bags they are colonizing in? The bag is slightly open and the lids are propped

EDIT: Good news guys, one of the buckets is pinning, the other isn't.  Im not sure why the other isn't, but from what I can tell the mycelium growing inside both looks generally the same.  I would assume they would've pinned at about the same time but the other might be behind a little bit.  I didn't really want to take them out of the bags they are in inside my house but will tomorrow so I can get a better view of the other one.

should I put the one that's pinning outside for fresh air?


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Edited by firstTIMER420 (10/19/15 01:47 AM)


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Re: pink oyster grow/questions [Re: firstTIMER420]
    #22401163 - 10/19/15 04:10 AM (8 years, 3 months ago)

Mushrooms do what they want. So one might pin now, and the other may take a week longer. You never know. You can put the pinning one outside, just make sure to mist several times a day, and don't put it in direct sunlight.


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Re: pink oyster grow/questions [Re: Jim Morrison]
    #22401533 - 10/19/15 08:15 AM (8 years, 3 months ago)

interesting.

When you say misting, i assume you mean take the lid off and mist the top as well as the holes where its pinning.


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Re: pink oyster grow/questions [Re: firstTIMER420]
    #22401979 - 10/19/15 09:37 AM (8 years, 3 months ago)

Just the pins. Leave the top on to keep the moisture inside


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Re: pink oyster grow/questions [Re: Jim Morrison]
    #22401981 - 10/19/15 09:38 AM (8 years, 3 months ago)

Pics are always good.


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Re: pink oyster grow/questions [Re: Jim Morrison]
    #22403145 - 10/19/15 11:35 AM (8 years, 3 months ago)

Mist the  bags and fruits. Don't open the bags up and spray the substrate. Once the bags are flushed out you can try hydrating the substrate again by hosing down the substrate.


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Re: pink oyster grow/questions [Re: Gr0wer]
    #22403315 - 10/19/15 12:21 PM (8 years, 3 months ago)

Quote:

Gr0wer said:
Mist the  bags and fruits. Don't open the bags up and spray the substrate. Once the bags are flushed out you can try hydrating the substrate again by hosing down the substrate.



Bags? I thought we're talking about buckets.


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Re: pink oyster grow/questions [Re: Jim Morrison]
    #22403342 - 10/19/15 12:27 PM (8 years, 3 months ago)

oh yea bucket, yea pop the lid and hose er down then let it drain after 2-3 flushes. Also i would like add i've recently moved away from buckets, they are harder to get pinset, pain to clean, and you can't see contams. The pinset is what did it for me. Now i use bags.


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Re: pink oyster grow/questions [Re: Gr0wer]
    #22404414 - 10/19/15 05:04 PM (8 years, 3 months ago)

gotcha guys

Ive put it outside in the shade, so it can get fresh air.  Im planning on misting it maybe once or twice a day, hopefully more pins pop up, and hopefully the other bucket starts pinning soon, if not since it sbeen about 10 or 11 days im going to dump it out. Made LC anyways,.

Gonna start pearl oysters as well, gonna look into using bags as well, what do you guys use for reference s far as technique for bags?


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Re: pink oyster grow/questions [Re: firstTIMER420]
    #22404820 - 10/19/15 06:32 PM (8 years, 3 months ago)

Quote:

firstTIMER420 said:


Gonna start pearl oysters as well, gonna look into using bags as well, what do you guys use for reference s far as technique for bags?




I'm lazy, so I use Lipa's PFP Tek. But not for pinks. They didn't seem to like the wood shavings. Works really well for lion's mane though. And am just now trying it with kings and blues. What are the current temps outside where you are?


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Re: pink oyster grow/questions [Re: Jim Morrison]
    #22410378 - 10/20/15 05:39 PM (8 years, 3 months ago)

its getting a little bit cooler now but still in the high 70s maybe low 80s

I will look at those


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Re: pink oyster grow/questions [Re: firstTIMER420]
    #22410587 - 10/20/15 06:27 PM (8 years, 3 months ago)

Pearl oyster and lion's mane would do really well at those temps.


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Re: pink oyster grow/questions [Re: Jim Morrison]
    #22410692 - 10/20/15 06:53 PM (8 years, 3 months ago)

LM is a cooler weather strain for good flavor, or so i've heard. Like 50-60.


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Re: pink oyster grow/questions [Re: Jim Morrison]
    #22411038 - 10/20/15 08:11 PM (8 years, 3 months ago)

well I have a completely colonized LC jar, of pearl oyster.  By the way, how long is a colonized LC jar good for?

im going to take some out tonight and test it on agar tomorrow. and then use it if its good.

what conditions do pink oyster prefer?


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OfflineGr0wer
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Re: pink oyster grow/questions [Re: firstTIMER420]
    #22411229 - 10/20/15 08:56 PM (8 years, 3 months ago)

colonized jars can store for weeks if not months in a fridge. Ive let colonized spawn sit in the jar for a week or two, typically you will see invitro pinning after a while but its still usable spawn.


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OfflinefirstTIMER420
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Re: pink oyster grow/questions [Re: Gr0wer]
    #22411433 - 10/20/15 09:48 PM (8 years, 3 months ago)

I knew you could store grain spawn in the fridge, jus thought LC's were different for some reason

I mistd the pins today and it seemed t help a little.

what do pink oysters taste like?


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12 34 http://www.shroomery.org/forums/showflat.php/Number/22386794[/url]
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Edited by firstTIMER420 (10/20/15 10:46 PM)


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OfflineGr0wer
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Re: pink oyster grow/questions [Re: firstTIMER420]
    #22411794 - 10/20/15 11:45 PM (8 years, 3 months ago)

oh i mis read. LC's, agar plate/slants, jars just about any culture can and should be stored in a fridge.

Pinks have a mild flavor, mostly takes on whatever you cook them with. They can be a tad bit tougher than browns and blues but the nice thing is they have almost no stem. With the temps cooling down you should only be growing pinks for a few more weeks before swapping to blues or other cold weather crops.

What do you plan on fruiting in?


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OfflinefirstTIMER420
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Re: pink oyster grow/questions [Re: Gr0wer]
    #22417446 - 10/22/15 09:52 AM (8 years, 3 months ago)

GOtcha, I usually store my other stuff in the fridge but I am just starting to get into LC's

Ok, they are coming along well ever since I started misting them outside. doubling in size every 12 hours or so.  But the othjer bucket is still sitting there doing nothing, I think it might be contamed.  Not sure what cobweb would look like on them but I guess I might be able to see :shrug:

I appreciate the help so far guys, my first time growing edibles, and I have all the magic mushies I could ever need so im starting to transition into edibles, and maybe make a business out of it!


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Re: pink oyster grow/questions [Re: firstTIMER420]
    #22417564 - 10/22/15 10:27 AM (8 years, 3 months ago)

If your storing pink oyster under refrigeration your risking killing your culture. :sad:


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OfflineJim Morrison
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Re: pink oyster grow/questions [Re: poofterFroth]
    #22417596 - 10/22/15 10:40 AM (8 years, 3 months ago)

Quote:

poofterFroth said:
If your storing pink oyster under refrigeration your risking killing your culture. :sad:




Very true. I had some spawn arrive in the mail and it got really cold, possibly even frozen. Didn't grow.


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Re: pink oyster grow/questions [Re: Jim Morrison]
    #22418783 - 10/22/15 03:19 PM (8 years, 3 months ago)

well the pink oyster I have left is out in LC but the pearl oyster and shitake LC syringes are in the fridge... I should take out?

I figured it would help until I used, don't know how long until im going to use them again.

By the way, do you guys clone oysters? I didn't get it them started on agar when I first had them,


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12 34 http://www.shroomery.org/forums/showflat.php/Number/22386794[/url]
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Edited by firstTIMER420 (10/22/15 03:22 PM)


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Re: pink oyster grow/questions [Re: firstTIMER420]
    #22419430 - 10/22/15 06:08 PM (8 years, 3 months ago)

Shiitake and pearl oyster are ok to refrigerate.

Cloning is cool to. :super:


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Re: pink oyster grow/questions [Re: poofterFroth]
    #22474246 - 11/04/15 01:18 AM (8 years, 2 months ago)

currently have my pearl oyster syringe in the fridge, although I have 5 LC jars and made two syringes each, so 2 syringes of pink oytster and 2 syringes of pearl oyster, but those are out in the open.


The bucket is flushing again, but the other one got trich early on, I think its this Florida heat+humidity.


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