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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: First Agar plates/test pictures. And then a question [Re: Ro0k]
#22346776 - 10/07/15 06:21 PM (8 years, 3 months ago) |
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That last plate looks like hella bacteria colonies.
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mushpunx
Fungus Punk



Registered: 04/20/14
Posts: 13,394
Last seen: 11 days, 10 hours
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Re: First Agar plates/test pictures. And then a question [Re: Pastywhyte]
#22347240 - 10/07/15 07:51 PM (8 years, 3 months ago) |
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Quote:
Pastywhyte said: That last plate looks like hella bacteria colonies.
Yea I second this it looks like no germination, but bacteria colonies completely following your streaks
You used a swab? Was it sterile, like opened from sterile packaging or wrapped in foil and run thru PC cycle?
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 Amateur Mycologists United AMU Q&A
Edited by mushpunx (10/07/15 07:52 PM)
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Ro0k
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Registered: 08/29/15
Posts: 95
Last seen: 6 years, 3 months
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Re: First Agar plates/test pictures. And then a question [Re: mushpunx]
#22347384 - 10/07/15 08:13 PM (8 years, 3 months ago) |
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Yeah. Opemed from a pack of sterile swabs. I was afraid of this. Hrmmm.... Into isolation it goes, then.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: First Agar plates/test pictures. And then a question [Re: Ro0k]
#22347394 - 10/07/15 08:16 PM (8 years, 3 months ago) |
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I doubt anything is going to germinate in that.
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mushpunx
Fungus Punk



Registered: 04/20/14
Posts: 13,394
Last seen: 11 days, 10 hours
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Re: First Agar plates/test pictures. And then a question [Re: Ro0k]
#22347396 - 10/07/15 08:17 PM (8 years, 3 months ago) |
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Quote:
Ro0k said: Yeah. Opemed from a pack of sterile swabs. I was afraid of this. Hrmmm.... Into isolation it goes, then.
Isolation? No its not gunna spread to the other plates. But toss it, you dont even have germination and already have that much bacteria. I think its un redeeemable man
Did you make the syringe yourself?
Ah Pasty beat me
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 Amateur Mycologists United AMU Q&A
Edited by mushpunx (10/07/15 08:17 PM)
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Ro0k
Stranger

Registered: 08/29/15
Posts: 95
Last seen: 6 years, 3 months
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Re: First Agar plates/test pictures. And then a question [Re: mushpunx]
#22347641 - 10/07/15 08:56 PM (8 years, 3 months ago) |
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Quote:
mushpunx said:
Quote:
Ro0k said: Yeah. Opemed from a pack of sterile swabs. I was afraid of this. Hrmmm.... Into isolation it goes, then.
Isolation? No its not gunna spread to the other plates. But toss it, you dont even have germination and already have that much bacteria. I think its un redeeemable man
Did you make the syringe yourself?
Ah Pasty beat me
Nope. Actually the first two wrapped in micropore are from a syringe I made. So that seems to have worked okay. The lower one is from a syringe I bought. I'm going to try again tomorrow on another plate and remove the swab from the equation. See if it's the syringe.
What do you think of if I sterilize a knife (I use a propane torch) then score the centre of the plate about half an inch (or cut a shallow x) and put a drop in there? Figure it'll work?
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mushpunx
Fungus Punk



Registered: 04/20/14
Posts: 13,394
Last seen: 11 days, 10 hours
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Re: First Agar plates/test pictures. And then a question [Re: Ro0k]
#22348973 - 10/08/15 07:38 AM (8 years, 3 months ago) |
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That'd be fine too I guess , swabbing it on just keeps the liquid from ro. Dring around and uses way less solution lowering your chance of contams
You can also use an inoculation loop, you can make one out of wire and an e xacto knife handle even. Flame sterilize it, cool it on the agar, flame your needle, cool it by squirting some liquid out, drip onto loop, swipe on agar.
Im just saying you don't want the liquid all over the place and the myc growing over any bacteria
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 Amateur Mycologists United AMU Q&A
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