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Shop: Mushroom-Hut Liquid Cultures   North Spore Injection Grain Bag

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InvisiblePastywhyteMDiscord
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Re: First Agar plates/test pictures. And then a question [Re: Ro0k]
    #22346776 - 10/07/15 06:21 PM (8 years, 3 months ago)

That last plate looks like hella bacteria colonies.


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Offlinemushpunx
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Re: First Agar plates/test pictures. And then a question [Re: Pastywhyte]
    #22347240 - 10/07/15 07:51 PM (8 years, 3 months ago)

Quote:

Pastywhyte said:
That last plate looks like hella bacteria colonies.




Yea I second this it looks like no germination, but bacteria colonies completely following your streaks

  You used a swab? Was it sterile, like opened from  sterile packaging or wrapped in foil and run thru PC cycle?


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Edited by mushpunx (10/07/15 07:52 PM)


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OfflineRo0k
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Re: First Agar plates/test pictures. And then a question [Re: mushpunx]
    #22347384 - 10/07/15 08:13 PM (8 years, 3 months ago)

Yeah. Opemed from a pack of sterile swabs. I was afraid of this. Hrmmm.... Into isolation it goes, then.


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InvisiblePastywhyteMDiscord
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Re: First Agar plates/test pictures. And then a question [Re: Ro0k]
    #22347394 - 10/07/15 08:16 PM (8 years, 3 months ago)

I doubt anything is going to germinate in that.


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Offlinemushpunx
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Re: First Agar plates/test pictures. And then a question [Re: Ro0k]
    #22347396 - 10/07/15 08:17 PM (8 years, 3 months ago)

Quote:

Ro0k said:
Yeah. Opemed from a pack of sterile swabs. I was afraid of this. Hrmmm.... Into isolation it goes, then.





Isolation? No its not gunna spread to the other plates. But toss it, you dont even have germination and already have that much bacteria. I think its un redeeemable man

Did you make the syringe yourself?

Ah Pasty beat me


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Edited by mushpunx (10/07/15 08:17 PM)


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OfflineRo0k
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Re: First Agar plates/test pictures. And then a question [Re: mushpunx]
    #22347641 - 10/07/15 08:56 PM (8 years, 3 months ago)

Quote:

mushpunx said:
Quote:

Ro0k said:
Yeah. Opemed from a pack of sterile swabs. I was afraid of this. Hrmmm.... Into isolation it goes, then.





Isolation? No its not gunna spread to the other plates. But toss it, you dont even have germination and already have that much bacteria. I think its un redeeemable man

Did you make the syringe yourself?

Ah Pasty beat me




Nope.  Actually the first two wrapped in micropore are from a syringe I made.  So that seems to have worked okay.  The lower one is from a syringe I bought.  I'm going to try again tomorrow on another plate and remove the swab from the equation.  See if it's the syringe.

What do you think of if I sterilize a knife (I use a propane torch) then score the centre of the plate about half an inch (or cut a shallow x) and put a drop in there?  Figure it'll work?


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Re: First Agar plates/test pictures. And then a question [Re: Ro0k]
    #22348973 - 10/08/15 07:38 AM (8 years, 3 months ago)

That'd be fine too I guess , swabbing it on just keeps the liquid from ro. Dring around and uses way less solution lowering your chance of contams

You can also use an inoculation loop, you can make one out of wire and an e xacto knife handle even.
Flame sterilize it, cool it on the agar, flame your needle, cool it by squirting some liquid out, drip onto loop, swipe on agar.

Im just saying you don't want the liquid all over the place and the myc growing over any bacteria


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