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Offlinerayuk
deja Vu
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Registered: 08/31/15
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Last seen: 8 years, 26 days
LB Agar bad or Bad Syringe?
    #22332524 - 10/04/15 12:39 PM (8 years, 3 months ago)

Hey everyone curious if someone with more experience might help me out. I have a few LB agar in dishes and I tried 2 syringes I have. Both of them where stored together in the fridge for some time now.

All the dishes began to smell bad and where the liquid was dropped creates a yellow looking fluid no white. Did I miss that LB will not work with mushies or are my syringes possibly bad?

I injected several WBS jars with both syringes as well had over 3 weeks with no growth, contam or mushie so it makes me think something is up with the syringes possibly.

I am fruiting atm, using the I should be able to place a tissue sample on the petri dish correct? That would allow me to test sterility of the dishes.

Petri Dishes & WBS Jars - Needle was flamed and swabbed. Everything done in a glove box.


Edited by rayuk (10/04/15 12:43 PM)


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Invisibleblindingleaf
blue collar underworld
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Registered: 07/19/13
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Re: LB Agar bad or Bad Syringe? [Re: rayuk]
    #22332588 - 10/04/15 12:53 PM (8 years, 3 months ago)

if LB has salt in it, cubes will do weird shit.  makes them fuzz up in a weird way.

post a picture of the plate.  they can store pretty long in syringe>fridge, but if u got them from a crappy source, they may not have been worth storing in the first place :frown:


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Offlinerayuk
deja Vu
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Registered: 08/31/15
Posts: 12
Last seen: 8 years, 26 days
Re: LB Agar bad or Bad Syringe? [Re: rayuk]
    #22332748 - 10/04/15 01:29 PM (8 years, 3 months ago)

Threw out the plates sadly, kicking myself for no image of them. Smelled horrible though just after 3 days. One syringe was unknown source other was from hawkseye. My previous syringe worked great from there.


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