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InvisibleMESCALATO
hunting.
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Registered: 05/16/02
Posts: 1,025
Loc: Central Alabama
when makeing a clone.....
    #2232797 - 01/10/04 04:30 AM (20 years, 1 month ago)

if one were to attempt to clone a GT fruit to pda agar would you suggest useing a piece of tissue from the base of the stem or the in side of the cap or is there no real differance? just want to hear from others experiances.


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OfflineGranola
bag lady

Registered: 05/18/03
Posts: 411
Loc: 50.0N-6.0E
Last seen: 18 years, 5 months
Re: when makeing a clone..... [Re: MESCALATO]
    #2233004 - 01/10/04 10:03 AM (20 years, 1 month ago)

I do both, best way for stems is to remove te cap, rip the stem lengthwise and remove a section in the center, it's the best probability for sterility. the cap works the same way


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InvisibleYarry
Old Timer
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Registered: 01/04/04
Posts: 23,762
Re: when makeing a clone..... [Re: Granola]
    #2233005 - 01/10/04 10:04 AM (20 years, 1 month ago)

sorry guys, im new to the concept of cloning mushrooms.. how is it done and why?


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Grumpy Old Man.


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Offlineforevrgrounded98
Stranger

Registered: 10/25/03
Posts: 283
Last seen: 17 years, 17 days
Re: when makeing a clone..... [Re: Yarry]
    #2233115 - 01/10/04 11:33 AM (20 years, 1 month ago)

Its done to isolate the characteristics of that individual mushroom. To do it, you remove a check of the inner stem or cap and grow it out using agar or stick it in a jar with an acceptable medium and it should grow.
-matt


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"If I don't see ya before the end of this one, I'll meet ya in the next one and don't be late" - Jimi Hendrix


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InvisibleYesItsMe
Homeless GOHOME !...
Registered: 10/29/03
Posts: 253
Loc: Working for Piss ;...
Re: when makeing a clone..... [Re: Yarry]
    #2233121 - 01/10/04 11:37 AM (20 years, 1 month ago)

Well , go here (Anno's tek).


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God save the Queen


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