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micro
bunbun has a gungun



Registered: 05/09/03
Posts: 7,532
Loc: Brick City
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Re: So I hear it's hard to print PE? [Re: mushpunx]
#22329930 - 10/03/15 07:35 PM (8 years, 3 months ago) |
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I would say you shouldn't do LC without something to sterilize it.
If you get septum-top bottles though, it's pretty easy to be sterile once you can PC the media.
If you need sterile spores you could always try fruiting it in a bag...
Does PE not produce them at all, or just very few?
Very few is okay but then you would certainly want agar since inoculating substrate with that would be painfully slow.
-------------------- Any research paper or book for free (Avatar is Maxxy, a character by Mizzyam, RIP)
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Fantastic Mr. Fox
Grass Seed Connoisseur


Registered: 02/12/15
Posts: 3,225
Loc:
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Re: So I hear it's hard to print PE? [Re: YaMoonSun]
#22329957 - 10/03/15 07:42 PM (8 years, 3 months ago) |
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Just because you see growth doesn't mean it's clean growth.
-------------------- Giving is all we have, for we're just grateful to be alive

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Grey
⇜ ✯ ⇝



Registered: 11/06/14
Posts: 6,223
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Re: So I hear it's hard to print PE? [Re: micro]
#22329967 - 10/03/15 07:45 PM (8 years, 3 months ago) |
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They produce them, they just don't drop them.
--------------------
AMU Q&A If you don't have a plan of your own, you'll become a part of somebody else's.
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YaMoonSun
The Double Standard


Registered: 10/23/14
Posts: 3,967
Loc: NY
Last seen: 5 months, 11 days
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Amen, but I'd like to take my chances. Worst possible outcome?
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: So I hear it's hard to print PE? [Re: YaMoonSun]
#22330417 - 10/03/15 09:38 PM (8 years, 3 months ago) |
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Quote:
YaMoonSun said: If I can't print it, I should still be able to empty a syringe of sterile water into a cap and quickly stir it up and pull it back into the syringe, no? Bad idea? Ever try it? Likely need to get a SAB for that procedure. 
it has worked out before, i'd expect a little failure along the way though(should have a sab for everything)
--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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invitro

Registered: 05/03/13
Posts: 2,529
Last seen: 1 month, 21 days
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Re: So I hear it's hard to print PE? [Re: YaMoonSun]
#22330675 - 10/03/15 10:40 PM (8 years, 3 months ago) |
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good info Grey, thx
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Violet



Registered: 12/06/11
Posts: 4,205
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Re: So I hear it's hard to print PE? [Re: invitro]
#22331287 - 10/04/15 03:25 AM (8 years, 3 months ago) |
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That's cool, about the centrifuge.
I have used the syringe drops method to harvest PE spores before into a syringe. I found it more tedious than using a swab but equally, possibly more, rewarding. Thing is the droplet's surface tension stops it from easily getting down into the gills. And when it does, it's not coming back out. So what I did was: 1. Sterilized a syringe with 5-8 mL of water in it, with the needle attached, all wrapped in foil. 2. Used my extra mature and wrinkly PE fruit that had grown invitro - picked in my sterile airflow work space, and readied the syringe in my other hand. 3. Placed a few droplets of water on the gills while holding the fruit upside down. 4. Worked the water into the gills with the tip of the needle. Repeated 3 and 4 until excess water that could easily be sucked up was building up in the cap. 5. Without absolutely shredding the gills, ran the needle tip along the gills, with the goal to rub off spores into the water. 6. Sucked as much of the water up as I could.
If you feel good about having this all kept clean during the process, which I did in my sterile airflow, you can shake the syringe and put water back in to stir it with the water that remained from the first time and try to get more of the spores that you freed but were left behind.
To me, this was worth it. Because with 1mL of spore solution I can start like 10 of my grain petris for my culture tech.
-------------------- Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it! PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers The simplest, quickest, safest tek! For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers! • Violet's Teks and Posts •
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YaMoonSun
The Double Standard


Registered: 10/23/14
Posts: 3,967
Loc: NY
Last seen: 5 months, 11 days
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Re: So I hear it's hard to print PE? [Re: Violet]
#22331992 - 10/04/15 10:05 AM (8 years, 3 months ago) |
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