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Offlineragadinks
MrBeatle
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liquid innoculation with which species ?
    #2232877 - 01/10/04 08:28 AM (12 years, 10 months ago)

I have tried to make liquid innoculation with following species:
- pleurotus pulmonaris
- flammulina velutipes
- p. azurescens
- shiitake
- hypholoma capnoides

either I have tried out the honey or a dextrose/yeast mixture for the liquid broth and inncoulated either WBS or sawdust spawn or beer soaked cardboard.
This were the results:
- pleurotus pulmonaris: mycelium on WBS ok / not tried to innoculate sawdust spawn / cardboard worked very well
- flammulina velutipes: mycelium on WBS ok / mycelium on sawdust spawn
- p. azurescens: no growth on WBS / not tried to innoculate sawdust spawn
- shiitake: not tried WBS / no growth on sawdust spawn
- hypholoma capnoides: innoculated sawdust and WBS but too early for results

It migth be that the innoculation of some species failed due to my lack of experience. So I wonder what are the experiences of other growers ? What did you use for liquid innoculum, which species did you innoculate on which substrate and was it successful ?


Edited by ragadinks (01/10/04 06:40 PM)


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Offlinebonsai
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Re: liquid innoculation with which species ? [Re: ragadinks]
    #2233352 - 01/10/04 04:16 PM (12 years, 10 months ago)

If you could explain your procedures maybe some one may find out what is going wrong. The Shiitake should have took to the sawdust. H"ow do you make your liquid innoculant?
bonsai


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OfflineSuntzu
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Re: liquid innoculation with which species ? [Re: ragadinks]
    #2233539 - 01/10/04 06:23 PM (12 years, 10 months ago)

I use a sort of liquid method. . .it doesn't involve growing the organism out in liquid culture, but still provides some of the benefit of liquid innoculum. In essence, a small chunk of colonized agar [or mycelium scraped off the surface] is added to a sterile tube of sterile water [usually about 10 mL in a 15 mL tube]. This is shaken, shaken, and shaken again, then used as soon as possible. There are better ways, but this is easy and has worked with every species I've tried--much better take-off than the agar chunk(s) alone.


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Offlineragadinks
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Re: liquid innoculation with which species ? [Re: bonsai]
    #2233557 - 01/10/04 06:38 PM (12 years, 10 months ago)

Quote:

bonsai said:
If you could explain your procedures maybe some one may find out what is going wrong. The Shiitake should have took to the sawdust. H"ow do you make your liquid innoculant?
bonsai




I mix either honey with water ( details see honey tek ) or mix 15 g dextrose and 1 gramm yeast with 0.5 liter water and sterilize it ( 20 minutes/ 121 degree Celsius ) and let it cool over night. Then I add a piece of mycelium to the broth ( in my pressure box ). Then I stir it with a stirrer for 4 - 10 days depending on species at about 24 degree Celsius. When I see that there is enough mycelium in the bottle I add about 20 - 30 ml to a jar of spawn. That's it.


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Invisiblemicro
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Re: liquid innoculation with which species ? [Re: ragadinks]
    #2236798 - 01/12/04 12:10 PM (12 years, 10 months ago)

I've used PDY for shiitake and it worked fine....

--
Micro


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Offlineragadinks
MrBeatle
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Re: liquid innoculation with which species ? [Re: micro]
    #2238432 - 01/13/04 05:31 AM (12 years, 10 months ago)

So at least shiitake should grow in liquid medium. How about P.azur ? Any experiences with other species ?


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Mushrooms, Mycology and Psychedelics >> Advanced Mycology

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