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Macavity224
Ubermensch



Registered: 06/09/11
Posts: 719
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Possible Contamination in LC?
#22327672 - 10/03/15 11:09 AM (8 years, 3 months ago) |
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I recently started an LC with a Penis Envy spore syringe, four days ago. Five jars were inoculated, 2cc's each. All the jars have what looks to be mycelium forming in them, but there are two that have small black "spots" floating around inside, which I believe is mold. I segregated those two jars from the other three as a precaution. Just looking for confirmation whether this is or is not mold.
Thanks. 

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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: Possible Contamination in LC? [Re: Macavity224]
#22327740 - 10/03/15 11:29 AM (8 years, 3 months ago) |
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Since you inoculated them from a spore syringe, it's safe to assume they're all contaminated, even the ones that don't look like it. That black spot is definitely bad unless it's a clump of spores, then it's bad because it's a huge clump of spores and probably carried some bacteria in.
All you can do is test the ones that don't look outright contaminated and hope they check out.
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Macavity224
Ubermensch



Registered: 06/09/11
Posts: 719
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Re: Possible Contamination in LC? [Re: Inocuole]
#22327784 - 10/03/15 11:43 AM (8 years, 3 months ago) |
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Why would the assumption be that they're all contaminated if they're from a spore syringe? Going from an already active LC would be preferable, but there isn't one.
-------------------- "O my soul, I taught you to say "Today" as well as "Once" and "Formerly," and to dance your dance over every here and there and yonder." Everything I post here is not true. Do not believe a single word of it.
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: Possible Contamination in LC? [Re: Macavity224]
#22327794 - 10/03/15 11:46 AM (8 years, 3 months ago) |
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I've put plenty of spores from syringes on agar and they are rarely 100% clean. Given how most prints are created, the likelihood of them being sterile is pretty slim. They would have to have been taken from a sterile fruit which don't produce nearly as many spores as a large well taken care of fruit, and thus are unlikely to be what the spore donors used.
Spores to LC is just bad news, I'd say the chances of contamination are easily greater than 75% for every jar of LC.
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Macavity224
Ubermensch



Registered: 06/09/11
Posts: 719
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Re: Possible Contamination in LC? [Re: Inocuole]
#22328136 - 10/03/15 01:07 PM (8 years, 3 months ago) |
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Fair enough. What is the best way to proceed in your opinion? I've tried spores to substrate twice with no results. The mold, or whatever I'm growing is the closest I've got to anything.
Is spore to LC really that unreliable? Or is the problem just using a spore syringe in the first place?
-------------------- "O my soul, I taught you to say "Today" as well as "Once" and "Formerly," and to dance your dance over every here and there and yonder." Everything I post here is not true. Do not believe a single word of it.
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: Possible Contamination in LC? [Re: Macavity224]
#22328162 - 10/03/15 01:11 PM (8 years, 3 months ago) |
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Right, now, you want to test your best looking LCs, maybe you got lucky. While that's going on, read this http://www.shroomery.org/forums/showflat.php/Number/19208976 and then use clean agar wedges to inoculate the LC, OR just start inoculating your jars with agar wedges, using those jars as masters, and G2G them to 10 more jars apiece. Or you can check out a Liquid Inoculant tek, which I've had good luck with personally. http://www.shroomery.org/forums/showflat.php/Number/20429745/fpart/26/vc/1#20429745
This helps with both of those: http://www.shroomery.org/forums/showflat.php/Number/21032261
Maybe you can even take one of those LCs, and inoculate agar with it, transfer away from the contams, and then use the resulting mycelium to inoculate a second LC so it's 100% clean.
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