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Violet



Registered: 12/06/11
Posts: 4,205
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Re: At what point do functional spores develop? [Re: SteveRogers]
#22328547 - 10/03/15 02:15 PM (8 years, 3 months ago) |
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Again? Pardon me, but I have no clue who you are. Maybe you just don't have a good answer. That's okay.
-------------------- Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it! PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers The simplest, quickest, safest tek! For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers! • Violet's Teks and Posts •
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SteveRogers
gandy dancer


Registered: 10/24/06
Posts: 3,450
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Re: At what point do functional spores develop? [Re: Violet]
#22328560 - 10/03/15 02:17 PM (8 years, 3 months ago) |
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glad to see you haven't lost that twinkle in your eye.
As I said, good luck OP
-------------------- "General, I am loyal to nothing......except The Dream"
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Violet



Registered: 12/06/11
Posts: 4,205
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Re: At what point do functional spores develop? [Re: SteveRogers]
#22328574 - 10/03/15 02:20 PM (8 years, 3 months ago) |
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Hahah, well, whatever. For what it's worth (which is a lot) my teks are the status quo now for ease and simplicity. One was even recently stickied a week for that exact reason. *shrug*
-------------------- Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it! PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers The simplest, quickest, safest tek! For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers! • Violet's Teks and Posts •
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Violet



Registered: 12/06/11
Posts: 4,205
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Re: At what point do functional spores develop? [Re: Violet]
#22328603 - 10/03/15 02:27 PM (8 years, 3 months ago) |
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Orca, are you still thinking about dropping a sporulatn cap in grain? I'd still suggest against it. Trade a spore print, in which case the best time is when the caps are well into being open. Even when you see spores on the upper stems. Then, as I suggested before, make a syringe for the culture tek. Unless I'm forgetting what you said after that...
Some strains will drop spores as soon as the veil is tearing. Others might wait until the cap is almost flat. Most are somewhere right in the middle. You'll quickly learn to know it when you see it!
-------------------- Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it! PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers The simplest, quickest, safest tek! For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers! • Violet's Teks and Posts •
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KauaiOrca
Waterman


Registered: 08/12/08
Posts: 3,131
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Re: At what point do functional spores develop? [Re: Violet]
#22329554 - 10/03/15 05:54 PM (8 years, 3 months ago) |
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Quote:
Violet said: Orca, are you still thinking about dropping a sporulatn cap in grain? I'd still suggest against it. Trade a spore print, in which case the best time is when the caps are well into being open. Even when you see spores on the upper stems. Then, as I suggested before, make a syringe for the culture tek. Unless I'm forgetting what you said after that...
Some strains will drop spores as soon as the veil is tearing. Others might wait until the cap is almost flat. Most are somewhere right in the middle. You'll quickly learn to know it when you see it!
Violet - I've got an absolutely fool proof method of generating spore prints/syringes that is 99% reliable in generating no contams. The cap never touches the surface of where the spores are dropping into.
Just for the hell of it, in my next MS grow, I'm going blend up a small developing mushroom that hasn't been exposed to any open air and inoculate a jar with it to see what happens. It's a 15 cent experiment just to see what happens. If it goes bad, BFD ... started this thread to see if, at 1/3 of its growth, the mushroom has spores in the cap that are essentially fully developed that would defeat the purpose.
-------------------- "The universe is endless, limitless and infinite. Any effort to define it's boundaries is an attempt to overcome ignorance. We are physical, mental and spiritual beings ... there is no beginning and there is no end. There is only memory. Our repeated loss of memory experiences create the illusion of beginnings and ends. Immortality is the ability to retain full memory through all consciousness transformations. Loss of memory is man's greatest curse and, in very real terms, death." -- Ancient Taoist Master
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LocN9ne
ɢᄋᄋd ԲᄋЯ ᄁᄋȚᅢΙᄁɢ ᄂᄋ₩ᄂΙԲᄐ



Registered: 04/17/15
Posts: 7,076
Loc: to the brain
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Re: At what point do functional spores develop? [Re: KauaiOrca]
#22329573 - 10/03/15 06:01 PM (8 years, 3 months ago) |
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The only way you are going to get a mushroom that has NEVER been touched by open air, is to grow it in vitro in a jar with the blender attachment on it the entire time then stick it to the blender without ever opening it... it would be more logical to do a regular slurry, no?
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Q&A US vs. THEM The more I learn, the less I know.
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KauaiOrca
Waterman


Registered: 08/12/08
Posts: 3,131
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Re: At what point do functional spores develop? [Re: LocN9ne]
#22329648 - 10/03/15 06:17 PM (8 years, 3 months ago) |
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Quote:
LocN9ne said: The only way you are going to get a mushroom that has NEVER been touched by open air, is to grow it in vitro in a jar with the blender attachment on it the entire time then stick it to the blender without ever opening it... it would be more logical to do a regular slurry, no?
Grow the mushroom invitro ... bring the jar into a SAB ... open it and pluck the mushroom ... chances of it being contam free should be pretty good, I'd guess but I could be wrong ... What the hell, worth a try ... not like I'm using it to spawn 50 grain jars or anything like that, just going to test it with one jar and see what happens. Might be a way to go straight to a new clone culture without going to agar. If it doesn't work, BFD.
-------------------- "The universe is endless, limitless and infinite. Any effort to define it's boundaries is an attempt to overcome ignorance. We are physical, mental and spiritual beings ... there is no beginning and there is no end. There is only memory. Our repeated loss of memory experiences create the illusion of beginnings and ends. Immortality is the ability to retain full memory through all consciousness transformations. Loss of memory is man's greatest curse and, in very real terms, death." -- Ancient Taoist Master
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Violet



Registered: 12/06/11
Posts: 4,205
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Re: At what point do functional spores develop? [Re: KauaiOrca]
#22329773 - 10/03/15 06:53 PM (8 years, 3 months ago) |
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Oh I see, you're not trying to get the spores, but to avoid them. Then yep that should work! That's pretty much a one-step clone slurry. It will work, and you actually CAN inoc tons of containers with it, just like the liquid inoculant tek.
Indeed, just like any sterile tranfer, still-air box work will make it plenty sterile. No need to grow in a blender attachment...
-------------------- Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it! PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers The simplest, quickest, safest tek! For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers! • Violet's Teks and Posts •
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LocN9ne
ɢᄋᄋd ԲᄋЯ ᄁᄋȚᅢΙᄁɢ ᄂᄋ₩ᄂΙԲᄐ



Registered: 04/17/15
Posts: 7,076
Loc: to the brain
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Re: At what point do functional spores develop? [Re: Violet]
#22329796 - 10/03/15 07:00 PM (8 years, 3 months ago) |
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Well there ya go... good luck on your venture...good vibes your way... and thank you violet.
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Q&A US vs. THEM The more I learn, the less I know.
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KauaiOrca
Waterman


Registered: 08/12/08
Posts: 3,131
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Re: At what point do functional spores develop? [Re: Violet]
#22330100 - 10/03/15 08:20 PM (8 years, 3 months ago) |
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Quote:
Violet said: Oh I see, you're not trying to get the spores, but to avoid them.
Exactly. And take advantage of the contamination free growing environment to the max to see if even one more step can be eliminated. Given the yield of just a few jars of even multispore of the strain I have, yield and efficiency, for me, is meaningless. If every once in a while I start a multi spore grow then just pluck 2-3 good looking developing mushrooms to start different clone cultures with clean mushroom slurries, I'm bound to stumble on one or two that's a prolific fruiter ... and maybe without agar at all. We'll see.
-------------------- "The universe is endless, limitless and infinite. Any effort to define it's boundaries is an attempt to overcome ignorance. We are physical, mental and spiritual beings ... there is no beginning and there is no end. There is only memory. Our repeated loss of memory experiences create the illusion of beginnings and ends. Immortality is the ability to retain full memory through all consciousness transformations. Loss of memory is man's greatest curse and, in very real terms, death." -- Ancient Taoist Master
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