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Offlineshroomfun
Stranger
Registered: 09/14/15
Posts: 21
Loc: Bay Area, CA
Last seen: 8 years, 3 months
Pasteurizing growing media
    #22326438 - 10/02/15 11:48 PM (8 years, 3 months ago)

Hello,

I understand that the growing medium / substrate (in my case compost) needs to be pasteurized before inoculating.

My question is why? When it will be inoculated and cased and left in open air?
Wouldn't contaminants affect the whole thing when left for open air?

I would like to understand the scientific reason behind pasteurizing the growing substrate.

Thanks.


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OfflinePabloSumgie
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Registered: 08/20/15
Posts: 84
Last seen: 8 years, 3 months
Re: Pasteurizing growing media [Re: shroomfun]
    #22326592 - 10/03/15 12:48 AM (8 years, 3 months ago)

Someone will chime in with a better answer but basically your killing the bad bacterias and what not but your leaving the beneficial ones begin to help with future contams.


--------------------
Alright then, picture this if you will:
10 to 2 AM, X, Yogi DMT, and a box of Krispy Kremes, in my "need to know" post, just outside of Area 51.
Contemplating the whole "chosen people" thing with just a flaming stealth banana split the sky like one would hope but never really expect to see in a place like this.
Cutting right angle donuts on a dime and stopping right at my Birkenstocks, and me yelping...
Holy fucking shit!


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Invisiblelipa

Registered: 07/24/07
Posts: 2,684
Trusted Cultivator
Re: Pasteurizing growing media [Re: shroomfun]
    #22327473 - 10/03/15 10:10 AM (8 years, 3 months ago)

1. flies and their eggs need to be killed to prevent instant infestation.
2. Yes it is vulnerable in the open air but the idea is to give the fungus a head start. Do realize there are other organism growing in your compost already. Some of them release enzymes that prevent the growth of the fungus you are trying to grow. Heat as well as preventing these organism from growing minimizes this issue.
3. After pasteurization most of the contaminates will be on top of the substrate from the open air like you said (You can cover the bed with plastic to help). They must grow inwards to infect the substrate.  You inoculate the substrate inside and hope that the fungus makes it to the top before the others make it below. Trichoderma will be one of the most common enemy you encounter. It releases enzymes that dissolve the cell walls of the mushroom hyphae inhibiting growth and preventing fruiting. When you pasteurize the substrate properly the bacteria and fungus you encourage to colonize the substrate at higher temperatures release enzymes that prevent the trichoderma from growing and provide an environment for the mushroom to grow as well as more food.


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Offlineshroomfun
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Registered: 09/14/15
Posts: 21
Loc: Bay Area, CA
Last seen: 8 years, 3 months
Re: Pasteurizing growing media [Re: lipa]
    #22330288 - 10/03/15 09:04 PM (8 years, 3 months ago)

Quote:

lipa said:
1. flies and their eggs need to be killed to prevent instant infestation.
2. Yes it is vulnerable in the open air but the idea is to give the fungus a head start. Do realize there are other organism growing in your compost already. Some of them release enzymes that prevent the growth of the fungus you are trying to grow. Heat as well as preventing these organism from growing minimizes this issue.
3. After pasteurization most of the contaminates will be on top of the substrate from the open air like you said (You can cover the bed with plastic to help). They must grow inwards to infect the substrate.  You inoculate the substrate inside and hope that the fungus makes it to the top before the others make it below. Trichoderma will be one of the most common enemy you encounter. It releases enzymes that dissolve the cell walls of the mushroom hyphae inhibiting growth and preventing fruiting. When you pasteurize the substrate properly the bacteria and fungus you encourage to colonize the substrate at higher temperatures release enzymes that prevent the trichoderma from growing and provide an environment for the mushroom to grow as well as more food.





Thanks for an excellent and enlightening answer.


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OfflineGr0wer
always improving
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Registered: 09/16/03
Posts: 6,056
Loc: El Paso, TX Flag
Last seen: 5 years, 10 months
Trusted Cultivator
Re: Pasteurizing growing media [Re: shroomfun]
    #22330363 - 10/03/15 09:26 PM (8 years, 3 months ago)

Durring colonization the uncolonized substrate is moist and prime envoinment for any existing bacteria, mold or fungus to bloom. By pasteurizing you make the substrate more neutral with competion for the mushroom growth.


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