|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
TheShroomanizer
Stranger-Danger


Registered: 06/12/09
Posts: 1,571
Loc: The Swamp
Last seen: 9 months, 1 day
|
WBS fully colonized Purple hue?
#22325989 - 10/02/15 09:13 PM (8 years, 3 months ago) |
|
|
Had 2 jars of WBS, they took off and did AMAZING...well, its been about 4 days at fully colonized and now on 1 of the jars it seems like the mycelium is turning a purplish/pinkish hue...it looks like normal mycelium, just changing from white to pinkish...It is hard to see in the pictures...anyone heard of this?
Here you can see its very healthy.
Here in the upper right side, you may be able to kind of see it...like the mycelium turning pink?
Bacteria? Mold taking over a fully colonized/consolodated jar?
-------------------- Trading Prints -Nature gave us one tongue and two ears so we could listen twice as much as we speak-
|
Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
|
|
Fusarium is white and looks exactly like myc. It will sporulate pink/purple when it's ready tho. Could it be fusarium? Hard to tell from the pics, but myc turning pink/purple is DEFINITELY a bad sign
|
TheShroomanizer
Stranger-Danger


Registered: 06/12/09
Posts: 1,571
Loc: The Swamp
Last seen: 9 months, 1 day
|
Re: WBS fully colonized Purple hue? [Re: Mad Season]
#22327650 - 10/03/15 10:59 AM (8 years, 3 months ago) |
|
|
Does it colonize the same speed? Because these took about 2 weekz
-------------------- Trading Prints -Nature gave us one tongue and two ears so we could listen twice as much as we speak-
|
Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
|
|
Yes almost exactly the same rate.
|
TheShroomanizer
Stranger-Danger


Registered: 06/12/09
Posts: 1,571
Loc: The Swamp
Last seen: 9 months, 1 day
|
Re: WBS fully colonized Purple hue? [Re: Mad Season]
#22327756 - 10/03/15 11:34 AM (8 years, 3 months ago) |
|
|
great. Are there any determining factors that I could be made aware of to tell the difference?
Like smell? Does it smell like earthy mycelium? or does it have a distinct odor?
-------------------- Trading Prints -Nature gave us one tongue and two ears so we could listen twice as much as we speak-
Edited by TheShroomanizer (10/03/15 11:37 AM)
|
Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
|
|
Quote:
Fusarium Sources: Soil, Plants, Unsterilized Grain, Humidifiers This mold has a white mycelium very similar to mushroom mycelium and produces bright colors commonly purple, pink, orange, and yellow. Color change commonly occurs after 3 weeks of growth and may not be seen on short-lived agar cultures. Fusarium may inhibit mushroom growth causing mushrooms to remain small with tiny caps and a brown discoloration inside the stem. This can occur even when the mold itself is not visible.
Fusarium contamination is most commonly seen in PF jars as a result of contaminated syringes. The mold looks like mushroom mycelium and grows at a similar speed. After a couple weeks, the jar being about 1/2 - 3/4 colonized the purplish color develops. The spores are generally not airborne but can stick to airborne dust and water droplets.
 
http://www.shroomery.org/forums/showflat.php?Cat=&Board=255&Number=17165834&PHPSESSID=&fpart=1#17165834
Basically when it's 100% give it a shake and recover. Make sure it fully colonizes cleanly within 48 hours after shaking.
I don't recommend sniffing or doing anything with fusarium. If you Google about it, lots of people go to the hospital because of the mycotoxins it produces. More than the black molds people worry about for sure. Anything turning pink, or that shows after a shake and recover gets tossed for sure
|
TheShroomanizer
Stranger-Danger


Registered: 06/12/09
Posts: 1,571
Loc: The Swamp
Last seen: 9 months, 1 day
|
Re: WBS fully colonized Purple hue? [Re: Mad Season]
#22327966 - 10/03/15 12:25 PM (8 years, 3 months ago) |
|
|
Yup doesn't look exactly like that..its a lighter pink...but normal mycelium is jet white so something up. I'll def be tossing them...damn I PCd for 90minutes wldve thought they wldve died. Maybe dirty syringe idk
-------------------- Trading Prints -Nature gave us one tongue and two ears so we could listen twice as much as we speak-
|
Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
|
|
Pcing would have killed any in there. It would be from an ineffective filter/bad lid, From the syringe, or slipped in when you inoculated/bad procedures.
I hope you don't wipe with alcohol after flaming the needle. You should flame between every inoculation(if you don't). Do you use a ship? Do you use a sab/FH? You should think about starting agar. How do you do your lids?
Edited by Mad Season (10/03/15 12:45 PM)
|
TheShroomanizer
Stranger-Danger


Registered: 06/12/09
Posts: 1,571
Loc: The Swamp
Last seen: 9 months, 1 day
|
Re: WBS fully colonized Purple hue? [Re: Mad Season]
#22328319 - 10/03/15 01:35 PM (8 years, 3 months ago) |
|
|
nah, i just wipe everything down real good, I do alluminum foil under lids, then tighten down the tops, and PC...then innoc through the pre-made holes on the lid-2-5 holes. and place filter paper over them. I flame sterilize between all inoculation points (red hot). I make home-made syringes though, so I am assuming it probably was on the print...as it turns out, of my 22 syringes, about 8 have contained contams of either Cobweb/Trich/ or this purple stuff. So ill just re-do my syringe method
-------------------- Trading Prints -Nature gave us one tongue and two ears so we could listen twice as much as we speak-
|
Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
|
|
Really? Not the placing unsterile filter paper over the holes you inoculated into? The lids are supposed to have the filter on them first, and go through the pc with it. The pc will sterilize the filter. What do you mean just wipe things down? Does that mean no to the sab?
So let me get this straight. You have open holes, in open air you inoculate lots of solution through, and then put filter paper onto (which I can guarantee isn't rated to .3 microns) in open air and you think it's the syringes? I can guarantee there's much more wrong than your syringes.
Read this. This is what we're fighting against: http://www.shroomery.org/forums/showflat.php/Number/21718811
There can be easily 5 million particles per cubic meter. Sometimes up to 10 billion. This is why sab, and proper technique is KEY.
Also with grains you only need 1 hole/ship. Tho I just pop the lid and inoculate in the sab. each grain jar gets MAX 3 drops down 1 side. This will greatly lower the chance of contaminations from the syringe. But won't hinder the cubes. Whether there's 1 million spores, or 50 million. Germination rates will be the same, much less contams have entered, and you still just shake at 20-30% to make it sky rocket
Edited by Mad Season (10/03/15 01:59 PM)
|
Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
|
Re: WBS fully colonized Purple hue? [Re: Mad Season]
#22328448 - 10/03/15 01:57 PM (8 years, 3 months ago) |
|
|
I highly recommend reading through the improve your sterile techniques link in my signature as well. If you want success every time, follow it to the t
|
TheShroomanizer
Stranger-Danger


Registered: 06/12/09
Posts: 1,571
Loc: The Swamp
Last seen: 9 months, 1 day
|
Re: WBS fully colonized Purple hue? [Re: Mad Season]
#22328470 - 10/03/15 02:01 PM (8 years, 3 months ago) |
|
|
Well, i do use a SAB, just probably not up to standards. and actually, this is laughable, but i used to use paper towels as 1st line defense to keep dust off the filter paper. But i guess with all of my mess ups I have been pretty lucky. I have 24 jars currently mid - fully colonized and 5 have shown contam. I used to actually have a pretty nice glove-box SAB that worked AMAZING, and then i moved and it somehow disappeared.
-------------------- Trading Prints -Nature gave us one tongue and two ears so we could listen twice as much as we speak-
|
Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
|
|
I used to put my lids upside down with absolutely no filter. I didn't use agar and inoculated 1-2ccs per jar. I still had some success. Looking back tho, the amount of success isn't even a quarter of today's work lol. Open air g2gs and shit... God idk how I did it.
They say don't fix whats not broken. The problem is, that once it starts to not work, it always doesn't work until you fix it. Doing it right tho means you got nothing to fix, and thus very rare contams. Hope you understand what I'm saying lol
|
TheShroomanizer
Stranger-Danger


Registered: 06/12/09
Posts: 1,571
Loc: The Swamp
Last seen: 9 months, 1 day
|
Re: WBS fully colonized Purple hue? [Re: Mad Season]
#22328521 - 10/03/15 02:09 PM (8 years, 3 months ago) |
|
|
yea haha, Fix my broke ass method before it fixes me? right?...
-------------------- Trading Prints -Nature gave us one tongue and two ears so we could listen twice as much as we speak-
|
Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
|
|
Haha yeah . But im not saying never go into the wrong. We should all experiment . But if you're fighting contams, following my signature link is the way to do it. It even says in the first paragraph it's to help people fight contams
|
TheShroomanizer
Stranger-Danger


Registered: 06/12/09
Posts: 1,571
Loc: The Swamp
Last seen: 9 months, 1 day
|
Re: WBS fully colonized Purple hue? [Re: Mad Season]
#22328555 - 10/03/15 02:16 PM (8 years, 3 months ago) |
|
|
Yes i see now. When you said click on your signature, I assumed you meant "Hookers and Blackjack"
Which actually made perfect sense
-------------------- Trading Prints -Nature gave us one tongue and two ears so we could listen twice as much as we speak-
|
Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
|
|
hookers and blackjack could be the answer too..
Good luck man. Ime prints in the sab come out clean af. Especially when you dilute them further in a syringe. I've been pretty dirty making my prints, and I've had nothing but praise on their cleanliness.
Ime, and even in labs too, 99% of contaminations are from humans. We're dirty af.
|
dmppb
On course


Registered: 11/11/11
Posts: 1,043
Loc: universe
Last seen: 2 months, 17 days
|
Re: WBS fully colonized Purple hue? [Re: Mad Season]
#22376669 - 10/14/15 12:38 AM (8 years, 3 months ago) |
|
|
hello , do you by any chance have pics of fusarium growing in straw bags ,
i ve gotten bright purple pink spots on my straw bags last couple of weeks ,
i'm just starting to do research on this one , how can i tell between lipstick , fusarium , pink mold etc.
thanks !
-------------------- ...and when you loose control ; you'll reap the harvest you have sown.
|
|