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OfflineAmishmedic8
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Agar dishes first time.
    #22324085 - 10/02/15 02:40 PM (8 years, 3 months ago)

http:/
First time working with agar. Sry the pics are as good as I can get. Its been really slow like 5wks. But the conditions arnt optimal and Ive read its a slow growing strain. It started off really white and fluffy. Very slowly its changed to an off white gray and only the one fuzzy patch. The one thats out of the sab got trich early on so I took it out cut out the contam and waiting it out. The other plates look very similar very white fluffy colonys but as it spreads it turns grayish. Wondering if it looks like cobweb or something else?


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OnlineNorthernerM
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Re: Agar dishes first time. [Re: Amishmedic8]
    #22325304 - 10/02/15 07:13 PM (8 years, 3 months ago)

There looks like there's several different thing in there to me. Hard to tell if any are the mycelium you're after though.


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InvisibleMad Season
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Re: Agar dishes first time. [Re: Northerner]
    #22327588 - 10/03/15 10:40 AM (8 years, 3 months ago)

:whathesaid: better pics! :P


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Invisiblemicro
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Re: Agar dishes first time. [Re: Mad Season]
    #22331111 - 10/04/15 01:19 AM (8 years, 3 months ago)

its not cobweb

if it is slow to grow I'd usually say chances are it is what you want, but holy shit 5 weeks

at what temperature you got those incubating


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OfflineAmishmedic8
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Re: Agar dishes first time. [Re: micro]
    #22354776 - 10/09/15 02:14 PM (8 years, 3 months ago)

Thanks for the input guys! Their at around the mid 70s but im working on increasing the temp. Heres the best pics I can get hope they are better!http:/ Thanks Again! And just to be sure since the plates are large and of questionable content would it be best to make an lc with the extremely fluffy "mycelium". Or can I just add to grain jars as is and cross my fingers? Maybe I have enough to do both?


--------------------
Well at least thats what my granmother would say, Medicine from the hinterland :thor:

Your vision will become clear only when you look into your heart ... Who looks outside, dreams. Who looks inside, awakens. - Carl Jung


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InvisibleMad Season
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Re: Agar dishes first time. [Re: Amishmedic8]
    #22354795 - 10/09/15 02:20 PM (8 years, 3 months ago)

Mid 70s is fine.

Also I'd do at least one more transfer to ensure cleanliness


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


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OfflineAmishmedic8
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Re: Agar dishes first time. [Re: Mad Season]
    #22355055 - 10/09/15 03:41 PM (8 years, 3 months ago)

Sounds good! It does look like its supposed to though right? The pics ive seen on here look more vine like in structure mine is extremely fluffy but as Ive never seen "good" myc..
For the transfers? I should take small slivers of the white, and transfer to multiple spots, on multiple plates as to "isolate". Sound right?
And does anyone have good pics of a contam on plates, Most I find have been deleted by poster.
Thanks again!


--------------------
Well at least thats what my granmother would say, Medicine from the hinterland :thor:

Your vision will become clear only when you look into your heart ... Who looks outside, dreams. Who looks inside, awakens. - Carl Jung


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