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DinkinFlicka84
Dude



Registered: 01/19/14
Posts: 546
Last seen: 5 years, 12 days
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Saving *7* bacterial bulk subs in GH? Best way to keep from contaminating?
#22323189 - 10/02/15 11:31 AM (8 years, 3 months ago) |
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Long story medium, my jars looked like this,
  
Each are different strains (Chitwan, Burma, B+, & Amazonian Clone) all from 4 diff. G2G, which were from multiple agar plates. I heated my closet too much one day and it was around 85f for a few hours in the closet, where normally it was ~78-80f consistently. This is when my healthy looking jars started looking like the pics above.
I know some say this is normal, but I've smelled the jars and they all smelled off, sour, or sweet. Not at all like the desired mushroomy-smell. I spawned 7 of these and forgot to smell them and wasn't paying attention to the tiny uncolonized spots on the grain (above). Despite that plus the smell, the bulk colonized very quickly (7 days) and were nice, solid subs, where even 1 has already fruited 2oz dry nicely!
It's surprising, b/c in the past, I've had more trouble with mold and bacteria than I would care to admit. One might think I was crazy for trying to so many god damn times with little to no fruits.
I want to keep all 7 subs from contaminating, since their bacteria makes them more prone to do so. Right? All I would think to do is to spread them out in my modified GH that holds 16 subs, to keep them from possibly contaminating each other IF 1 of them happened to get mold.
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champinhom
Lord Justhappensness


Registered: 03/06/09
Posts: 987
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Re: Saving *7* bacterial bulk subs in GH? Best way to keep from contaminating? [Re: DinkinFlicka84]
#22323419 - 10/02/15 12:18 PM (8 years, 3 months ago) |
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Do you really think that having some jars of cube myc at 85 degrees for a few hours is going to spoil them? If they have that much potential for bacterial contamination, then no matter what temp you kept them at, they would eventually conk out on you. A lot of the time I will put jars of cube myc in an incubator that gets to about 85 and keep them in there for days. No problem.
Anyway, those subs, as far as I can see, look like champs.
-------------------- My father used to say: I don't care what else you do in life, just don't be an asshole. People, forgive me when I forget what my daddy said. Cut back the proliferating list of people whose opinions can hurt you. Unless they have done or want to do you some good, their views are just not worth tracking. Saul Bellow “People are just cannibals unless they leave each other alone.” Doris Lessing Those whom the gods would save, they dower with compassion. Mr. P. Silocybin
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Re: Saving *7* bacterial bulk subs in GH? Best way to keep from contaminating? [Re: champinhom]
#22323457 - 10/02/15 12:26 PM (8 years, 3 months ago) |
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Don't you have another thread going with the same question asked and answered?
http://www.shroomery.org/forums/showflat.php/Number/22319196
Edited by Mad Season (10/02/15 12:27 PM)
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DinkinFlicka84
Dude



Registered: 01/19/14
Posts: 546
Last seen: 5 years, 12 days
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Re: Saving *7* bacterial bulk subs in GH? Best way to keep from contaminating? [Re: Mad Season]
#22326368 - 10/02/15 11:22 PM (8 years, 3 months ago) |
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Quote:
Mad Season said: Don't you have another thread going with the same question asked and answered?
http://www.shroomery.org/forums/showflat.php/Number/22319196
I realize they seem very similar, even with the pictures, but the pics were a necessity to help explain the situation. Also, by asking how to keep these potentially bad subs going, I got an answer that really helped and retrieved what I was looking for. Thanks, Champ!
The other thread is asking if I should spawn those jars. Which is quite close to this thread here, where I asked how do I keep these contaminated jars to have them fruit as well.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: Saving *7* bacterial bulk subs in GH? Best way to keep from contaminating? [Re: DinkinFlicka84]
#22326542 - 10/03/15 12:23 AM (8 years, 3 months ago) |
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again i will say those do not look to be infected to a point of worry, look up bacilus or coli in grain spawn
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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DinkinFlicka84
Dude



Registered: 01/19/14
Posts: 546
Last seen: 5 years, 12 days
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Re: Saving *7* bacterial bulk subs in GH? Best way to keep from contaminating? [Re: cronicr]
#22329845 - 10/03/15 07:14 PM (8 years, 3 months ago) |
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Quote:
cronicr said: again i will say those do not look to be infected to a point of worry, look up bacilus or coli in grain spawn
This one sub did great, still with no contamination. However, the other subs haven't started pinning, but also no moldy spots. I am surprised that jars with the uncolonized spots on the grain were able to be spawned and make fruits like this. Especially this big on the first flush! This size is the about the same as a 2nd or 3rd flush for me.
I dont' know what strain this is, b/c I forgot to label the subs, so if you can me if these are either Amazonian, Chitwan, Burma or B+ ( i'm pretty sure they're not B+ cuz I don't think I had any jars with B+ at the time.
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MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
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Re: Saving *7* bacterial bulk subs in GH? Best way to keep from contaminating? [Re: DinkinFlicka84]
#22330112 - 10/03/15 08:23 PM (8 years, 3 months ago) |
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Your fine. I've used a lot of verry bacterial spawn. Yours doesn't look bad. If you are keep your FAE up bacteria isn't the end of the world.
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DinkinFlicka84
Dude



Registered: 01/19/14
Posts: 546
Last seen: 5 years, 12 days
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Re: Saving *7* bacterial bulk subs in GH? Best way to keep from contaminating? [Re: MudaFuka]
#22335463 - 10/05/15 01:43 AM (8 years, 3 months ago) |
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Quote:
MudaFuka said: Your fine. I've used a lot of verry bacterial spawn. Yours doesn't look bad. If you are keep your FAE up bacteria isn't the end of the world.
Just to get this bacteria issue completely understood...
The bacteria doesn't directly affect the substrate. However, it leaves bulk more vulnerable to molds, more bacteria, etc... I have a cool mist piped in at the top right that blows almost 24/7 to help with the vulnerable subs more protected from mold spores settling and infecting my subs.
**Can someone remind me why or how the FAE keep mold out. Isn't FAE the reason why some mold, especially trich that doesn't show up until fruiting? In the tub for 8 days inside of a liner, the sub doesn't hardly ever get green by the time I move it into the GH.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: Saving *7* bacterial bulk subs in GH? Best way to keep from contaminating? [Re: DinkinFlicka84]
#22335467 - 10/05/15 01:46 AM (8 years, 3 months ago) |
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molds can thrive in a stale air enviroment
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
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Re: Saving *7* bacterial bulk subs in GH? Best way to keep from contaminating? [Re: cronicr]
#22335714 - 10/05/15 05:53 AM (8 years, 3 months ago) |
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Bacteria does directly affect the substrate. It generally loweres yeilds considerably. It can also cause deformed looking fruits. On the plus side bacteria quite often causes the substrate to Stat producing pins faster. The reason bacteria isn't the end of the world is. Bacteria competes with your cube mycelium for space. Where as mold feeds on your mycelium.
No FAE isn't the cause of mold. You spawn your sub in open air. It already has tons of mold spores mixed into it. A few more landing on top isn't going affect much.
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DinkinFlicka84
Dude



Registered: 01/19/14
Posts: 546
Last seen: 5 years, 12 days
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Re: Saving *7* bacterial bulk subs in GH? Best way to keep from contaminating? [Re: MudaFuka]
#22336451 - 10/05/15 11:05 AM (8 years, 3 months ago) |
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Quote:
MudaFuka said: Bacteria does directly affect the substrate. It generally loweres yeilds considerably. It can also cause deformed looking fruits. On the plus side bacteria quite often causes the substrate to Stat producing pins faster. The reason bacteria isn't the end of the world is. Bacteria competes with your cube mycelium for space. Where as mold feeds on your mycelium.
No FAE isn't the cause of mold. You spawn your sub in open air. It already has tons of mold spores mixed into it. A few more landing on top isn't going affect much.
I love learning more shit about this hobby. Thanks for your input. I'm guessing that bacteria doesn't grow/spread too fast, since the myc usually colonized the entire sub and the bacteria doesn't have any significant section of the sub. I'm trying to get rid of all or most bacteria I've had in my spawn/subs extra. I'm sure it's my[. '
Any tips tips for avoiding bad spawn? I've stopped using a heater in my closet that would keep the temp to 80f, which was fine, right? Does anyone keep their "jar room" at 80f or more?
I use an SAB and almost an entire large bottle of 70% isopropyl for my hands, wiping down he SAB, top, sides, and bottom paper towels that are also soaked and line the bottom. I cover the walls/bottom of my SAB and I flame sterilize with a small blow torch before every inoculation (if it's a print/MS). Normally I knock up my jars with agar, so I can see if there is any contamination, which I've read can be invisible and odorless. Great!
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bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Saving *7* bacterial bulk subs in GH? Best way to keep from contaminating? [Re: DinkinFlicka84]
#22336455 - 10/05/15 11:06 AM (8 years, 3 months ago) |
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Misting spreads bacteria like mad.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: Saving *7* bacterial bulk subs in GH? Best way to keep from contaminating? [Re: bodhisatta]
#22336502 - 10/05/15 11:21 AM (8 years, 3 months ago) |
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i keep my jars in a room between 70-75
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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