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OfflineRo0k
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Registered: 08/29/15
Posts: 95
Last seen: 6 years, 3 months
Better way to make a syringe?
    #22322461 - 10/02/15 07:43 AM (8 years, 3 months ago)

I have a question about syringe technique because I think I goofed while making my first syringe last night.

Procedure: the day before I boiled water and did the suck it up and shoot it into the sink routine 6 times, then let cool overnight. Last night I got everything in my glove box, sprayed the surfaces down woth soapy water and wiped all my gear including the shotglass and outside of the spore print bag with alcohol. Let sit for a bit. Flamed my scalple and scraped a decent amount of spores into the shot glass. Flamed the needle and shot the sterile water into the glass and sucked it up.. Repeated a few times.

NOW, here's where I screwed up. I ended up sucking some air into the needle and when I examined the syringe I also see some fibres from the paper towel I used to sterilize floating around. I'm pretty sure this syringe is a wash, but I'll find out in a week once I check on agar. Plus it'll give me some practice and I won't feel bad about wasting aa viable syringe.

Assuming I don't have the money to buy one nor the equipment to build one, is there a better way without a flowhood? I was thinking some kind of bottle like they use for injectible meds but you'd still need to expose it to air to put the spores in.

Any ideas?


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Offlinetripdawg420
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Registered: 02/02/09
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Re: Better way to make a syringe? [Re: Ro0k]
    #22322533 - 10/02/15 08:13 AM (8 years, 3 months ago)

a lil air is fine when i made syringes i use to take one syringe of water and squirt out half of it in the glass so there be extra water so you wont suck air :thumbup:


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OfflinePussyFart
Retired Cultivation Extrodinaire
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Registered: 04/08/12
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Re: Better way to make a syringe? [Re: Ro0k]
    #22322536 - 10/02/15 08:14 AM (8 years, 3 months ago)

Quote:

Ro0k said:
I also see some fibres from the paper towel I used to sterilize floating around.



U say u flamed the needle, but then you say you sterilized with a paper towel?

You wiped the needle with alcohol after flaming didn't you? That is a mistake.


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OfflinetaGyo
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Registered: 10/16/14
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Re: Better way to make a syringe? [Re: PussyFart]
    #22322604 - 10/02/15 08:33 AM (8 years, 3 months ago)

Quote:

taGyo said:
Alochol SANITIZES, flames STERILIZE. Sterilization means NO germs, mold can survive sanitation.
Don't wipe your needle with alcohol after flame sterilization:

Quote:

swatt_haze said:
wiping a needle with alcohol after flame sterilizing is like rubbing shit on your hands after you washed them and eating a burger.





Flame until the needle is red hot. It will turn black first (because you're using a lighter), then slowly turn red.

Flame sterilize between each JAR, not each inoculation hole. .25 cc a hole for 1cc total each BRF jar.
Grains is 1CC as well.

After flame sterilization gently push down on the plunger. It'll shoot out steam until a drop of liquid comes out. Let the drop gently fall and then push your needle through the hole, making sure to angle it TOWARDS THE GLASS. I look down and check to make sure my needle is against the glass sometimes but you can feel it.

If you push and it feels difficult and nothing's coming out gently pull your needle upward while shooting. BE CAREFUL, this will immediately loosen up your needle and if you're pushing too hard you'll shoot way too many CCs into a hole.




I got my own copy/paste PF :laugh2:, see anything you'd change?


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OfflineRo0k
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Registered: 08/29/15
Posts: 95
Last seen: 6 years, 3 months
Re: Better way to make a syringe? [Re: PussyFart]
    #22322693 - 10/02/15 09:07 AM (8 years, 3 months ago)

Nope. THAT I know not to do. Used an alcohol soaked towel to sterilize the shotty, and I assume some fibers fell off. Prolly going to pc it next time and use more water so no air.


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OfflinePussyFart
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Re: Better way to make a syringe? [Re: Ro0k]
    #22322720 - 10/02/15 09:15 AM (8 years, 3 months ago)

Quote:

Ro0k said:
Nope. THAT I know not to do. Used an alcohol soaked towel to sterilize sanitize the shotty, and I assume some fibers fell off.



:thumbup:


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Offlinemushpunx
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Re: Better way to make a syringe? [Re: PussyFart]
    #22323072 - 10/02/15 11:00 AM (8 years, 3 months ago)

Flow hoods are awesome but almost anything you can do in front of a flow hood can be done just as successfully in a still air box if you learn proper sterile technique


I think the best way is still to knock some spores into sterile  water and suck up into syringe.

The only real reason for syringes is for BRF cakes. Anything else a print and a loop is far more desireable IMO


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Invisibletahoe
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Registered: 11/26/03
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Re: Better way to make a syringe? [Re: mushpunx]
    #22324342 - 10/02/15 03:30 PM (8 years, 3 months ago)

I used to make spore syringes the same way. There is a good chance that the syringe is clean even though there are paper towel fibers in it.

Next time use a disposable petri dish instead of a shot glass.

Get a few alcohol wipes and use those instead of paper towels. Less likely to leave fibers behind and they should be sterile if it does.


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Offlinekosmokratorshaman
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Registered: 05/13/15
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Re: Better way to make a syringe? [Re: tahoe]
    #22324377 - 10/02/15 03:38 PM (8 years, 3 months ago)

get denatured alcohol, and flip that same shotglass (that you are no longer using) upside down. add a little denatured alcohol to it, and light that. Now you have a clean flame that wont leave soot.


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Offlinemushpunx
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Re: Better way to make a syringe? [Re: tahoe]
    #22324643 - 10/02/15 04:53 PM (8 years, 3 months ago)

I like the idea of using the sterile pitre!


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Invisiblegizmo1
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Registered: 06/15/11
Posts: 3,831
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Re: Better way to make a syringe? [Re: mushpunx]
    #22324830 - 10/02/15 05:33 PM (8 years, 3 months ago)

I also make syringes using petri dishes. I posted about this in another thread and a TC agreed that he also likes to use them for syringe making I forgot which TC it was though?


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