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OfflineYaMoonSun
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Petri Question
    #22321326 - 10/01/15 09:32 PM (8 years, 3 months ago)

Is there a better alternative to parafilm, or is this information still legit? I want to get some petris very soon.


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OfflineYaMoonSun
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Re: Petri Question [Re: YaMoonSun]
    #22321351 - 10/01/15 09:40 PM (8 years, 3 months ago)

It doesn't go over the vents, right? Should I put micropore tape over the vents? Do I need both? :shrug:


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Re: Petri Question [Re: YaMoonSun]
    #22321396 - 10/01/15 09:53 PM (8 years, 3 months ago)

You need vented. Just wrap with saran.


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OfflineYaMoonSun
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Re: Petri Question [Re: Pastywhyte]
    #22321410 - 10/01/15 09:58 PM (8 years, 3 months ago)



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Re: Petri Question [Re: YaMoonSun]
    #22321488 - 10/01/15 10:23 PM (8 years, 3 months ago)

Ummm no. Not only is that a ripoff, there is no guarantee how well those were prepared or if the agar was even properly sterilized. Do things yourself and be confident they were done right.


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OfflineYaMoonSun
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Re: Petri Question [Re: Pastywhyte]
    #22321500 - 10/01/15 10:27 PM (8 years, 3 months ago)

They seem to have pretty decent reviews, but okay, you're more than likely right. I need to get a ton of supplies still then :facepalm3:


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Invisiblemicro
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Re: Petri Question [Re: Pastywhyte]
    #22321517 - 10/01/15 10:33 PM (8 years, 3 months ago)

Well, if it weren't sterile it would probably be culturing something by now, but it looks like LB media which is going to be preferential to bacteria anyway. I agree -- you're much better doing this yourself and using media that is specific to fungi, esp. basidiomycetes / dung lovers.

OP, if you are asking about parafilm, it is what they use in biological laboratories but we always threw the petris back in their sleeve and stored it in the walk-in fridge. I don't remember ever using that to culture them. Not sure if it is oxygen/co2 that is the reason; the dishes are often left upside-down and shut anyway, but I figured I'd mention it, for what it is worth.

(if I had to guess I'd say doesn't matter when culturing, but wrap it up to store)


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OfflineYaMoonSun
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Re: Petri Question [Re: micro]
    #22321544 - 10/01/15 10:42 PM (8 years, 3 months ago)

It's likely because of co2 being heavier, it just falls out of the petri as after the mold/fungi produces it, thanks for the response. Good tip. :cheers:


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Re: Petri Question [Re: YaMoonSun]
    #22321835 - 10/02/15 12:29 AM (8 years, 3 months ago)

co2 does not fall:thumbup:


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Re: Petri Question [Re: cronicr]
    #22322046 - 10/02/15 02:19 AM (8 years, 3 months ago)

U sure bro?!? I've seen it fall with my own 2 eyes son! :lol:


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OfflineYaMoonSun
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Re: Petri Question [Re: cronicr]
    #22323061 - 10/02/15 10:58 AM (8 years, 3 months ago)

Why wouldn't it if it's heavier than oxygen? Too much free air exchange mixing the co2 and oxygen up?


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InvisibleMad Season
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Re: Petri Question [Re: YaMoonSun]
    #22323117 - 10/02/15 11:12 AM (8 years, 3 months ago)

It's all these damn air pressures and currents! The world isn't a vacuum. Co2 and o2 would split if it was a vacuum.. Void of all air and currents.


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Re: Petri Question [Re: Mad Season]
    #22323156 - 10/02/15 11:21 AM (8 years, 3 months ago)

CO2 and O2 mix together. It's not an oil and water kind of deal.


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OfflineYaMoonSun
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Re: Petri Question [Re: taGyo]
    #22323249 - 10/02/15 11:43 AM (8 years, 3 months ago)

So those mycelium bags people put in their cannabis grow rooms to replace co2 tanks don't actually need to be elevated? Damn pot-heads. :pipesmoke:

Just for the record, and I know it may sound stupid, but I don't cover the vents with the saran, only the sides of the petris, correct?


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Offlineaerow.thefox
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Re: Petri Question [Re: taGyo]
    #22323259 - 10/02/15 11:47 AM (8 years, 3 months ago)

Quote:

taGyo said:
CO2 and O2 mix together. It's not an oil and water kind of deal.



im not sure im entirerly sure thats accurate. do they mix well and stay mixed in a normal enviroment? yea of course.

but they DO have different masses. and BOTH are gasses influenced by fluid dynamics. i would imagine if all gasses were trapped inside an air-tight container, with an even temperature throughout, the would naturally seperate into layers of gasses. with the most dense at bottom and least at top. without temperature or physical interaction causing disturbances in a material so easily moved... just my opinion. :shrug:


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OfflinetaGyo
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Re: Petri Question [Re: aerow.thefox]
    #22323535 - 10/02/15 12:40 PM (8 years, 3 months ago)

Yeah, in a hypothetical world with hypothetical exacts they may or may not segregate themselves based on density and other variables. I haven't done enough research to tell you.

But in an SGFC, Petri or Mono the CO2 isn't simply on one level of the SGFC, it's mixed with the oxygen.


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Re: Petri Question [Re: taGyo]
    #22323567 - 10/02/15 12:46 PM (8 years, 3 months ago)

Not to mention heat currents being formed by the mycelium.


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Offlineaerow.thefox
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Re: Petri Question [Re: Pastywhyte]
    #22323681 - 10/02/15 01:08 PM (8 years, 3 months ago)

exactly my point. in a normal enviroment they mix up just fine. too many variables. but they CAN seperate


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Re: Petri Question [Re: aerow.thefox]
    #22323703 - 10/02/15 01:12 PM (8 years, 3 months ago)

CO2 can fall real fast real hard. I dropped a CO2 tank on my toe once and lost the nail :lol:


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Offlinetripdawg420
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Re: Petri Question [Re: Pastywhyte]
    #22323708 - 10/02/15 01:13 PM (8 years, 3 months ago)

:shocked:


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Offlineaerow.thefox
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Re: Petri Question [Re: tripdawg420]
    #22323745 - 10/02/15 01:21 PM (8 years, 3 months ago)

:shocked2: ouch!


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OfflinetaGyo
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Re: Petri Question [Re: Pastywhyte]
    #22323758 - 10/02/15 01:24 PM (8 years, 3 months ago)

Quote:

Pastywhyte said:
CO2 can fall real fast real hard. I dropped a CO2 tank on my toe once and lost the nail :lol:



:laugh2:


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Re: Petri Question [Re: aerow.thefox]
    #22323808 - 10/02/15 01:36 PM (8 years, 3 months ago)

Quote:

aerow.thefox said:
im not sure im entirerly sure thats accurate. do they mix well and stay mixed in a normal enviroment? yea of course.

but they DO have different masses. and BOTH are gasses influenced by fluid dynamics. i would imagine if all gasses were trapped inside an air-tight container, with an even temperature throughout, the would naturally seperate into layers of gasses. with the most dense at bottom and least at top. without temperature or physical interaction causing disturbances in a material so easily moved... just my opinion. :shrug:




They would both take the shape of the container and would ideally be evenly dispersed. I say 'ideally' because nothing is ever 100% even. Think of it like a bunch of balls rolling around on a floor. You are projecting the 3D space to a 2D plane, and for simplicity's sake imagine they never stop. They would bounce around constantly hitting into each other from all directions. It's the same with two liquids in solution. You could put it in a centrifuge and it wont separate; if it does it was a suspention.

The reason people store petris upside-down is mostly so humidity doesn't cloud up the top.

As for "vents" I'm not sure what you mean; the only part of a petri dish you should need to seal is around the edges. Just take the parafilm and wrap it a few times around the edges, tightening it a bit (but not so much it will break).


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OfflinetaGyo
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Re: Petri Question [Re: micro]
    #22323822 - 10/02/15 01:39 PM (8 years, 3 months ago)

Honestly I just deal with it :shrug:, eventually the condensation (Not humidity) disperses as both temperatures reach equilibrium. Or it doesn't :shrug:, regardless I can spot myc.


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Re: Petri Question [Re: taGyo]
    #22323830 - 10/02/15 01:41 PM (8 years, 3 months ago)

yeah, it's not a necessity


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Re: Petri Question [Re: micro]
    #22323988 - 10/02/15 02:21 PM (8 years, 3 months ago)

Vented petri dishes have little raised sections on the rim of the bottom plate. This keeps the lid from sealing to the lower dish and forming and anerobic environment. Its so standard its often not even mentioned.


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OfflineYaMoonSun
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Re: Petri Question [Re: Pastywhyte]
    #22324066 - 10/02/15 02:36 PM (8 years, 3 months ago)

Well if heat rises and the petri dishes are upside down, wont the heat just be pushing the already falling co2 out of the dishes?


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Re: Petri Question [Re: YaMoonSun]
    #22324478 - 10/02/15 04:06 PM (8 years, 3 months ago)

A culture on a plate isn't going to generate a whole lot of heat. Actually is not going to consume a whole lot of O2 or produce a large amount of CO2 either.  But as was said earlier most of that will be a mix. It would be like dropping some dye in a tub of water and trying to push that to one side.


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Re: Petri Question [Re: Pastywhyte]
    #22324487 - 10/02/15 04:07 PM (8 years, 3 months ago)

Great analogy, I think that paints the picture perfectly :congrats:


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OfflineYaMoonSun
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Re: Petri Question [Re: taGyo]
    #22324502 - 10/02/15 04:15 PM (8 years, 3 months ago)

Well shit, okay. :cheers:
I still think the vents need micro pore tape :shrug:


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Re: Petri Question [Re: YaMoonSun]
    #22324577 - 10/02/15 04:38 PM (8 years, 3 months ago)

The vents are inside the plate when its closed up
The edge needs parafilm or saran. Tape would be a pita.


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OfflineYaMoonSun
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Re: Petri Question [Re: Pastywhyte]
    #22324597 - 10/02/15 04:42 PM (8 years, 3 months ago)

I've never handled them, I'm sure I'll know what you mean once my order arrives.


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Re: Petri Question [Re: Pastywhyte]
    #22324619 - 10/02/15 04:47 PM (8 years, 3 months ago)

Quote:

Pastywhyte said:
Vented petri dishes have little raised sections on the rim of the bottom plate. This keeps the lid from sealing to the lower dish and forming and anerobic environment. Its so standard its often not even mentioned.




haha, i never knew that was a vent. neat xD


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Offlinehamloaf
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Re: Petri Question [Re: Pastywhyte]
    #22324681 - 10/02/15 05:03 PM (8 years, 3 months ago)

Quote:

Pastywhyte said:
The vents are inside the plate when its closed up
The edge needs parafilm or saran. Tape would be a pita.



Indeed. 

One time Grey sent me some pasties of edible cultures with the lids wrapped in micropore tape.  A huge sticky PITA it was. 

Nice tid-bit of information over vented petri dishes, as well.


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Edited by hamloaf (10/02/15 05:23 PM)


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OfflineKizzle
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Re: Petri Question [Re: aerow.thefox]
    #22325544 - 10/02/15 07:46 PM (8 years, 3 months ago)

Quote:

aerow.thefox said:
Quote:

taGyo said:
CO2 and O2 mix together. It's not an oil and water kind of deal.



im not sure im entirerly sure thats accurate. do they mix well and stay mixed in a normal enviroment? yea of course.

but they DO have different masses. and BOTH are gasses influenced by fluid dynamics. i would imagine if all gasses were trapped inside an air-tight container, with an even temperature throughout, the would naturally seperate into layers of gasses. with the most dense at bottom and least at top. without temperature or physical interaction causing disturbances in a material so easily moved... just my opinion. :shrug:



You'd think so but you also might think salt would fall out of water.

Like salt water the tendency for areas with high concentrations of CO2 to diffuse into areas of lower concentration (Entropy) is dominant. Density is simply not an important consideration when dealing with gaseous solutions, even in an airtight container.
:themoreyouknow:


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Re: Petri Question [Re: Kizzle]
    #22325548 - 10/02/15 07:47 PM (8 years, 3 months ago)

:rockon:


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Re: Petri Question [Re: YaMoonSun]
    #22325593 - 10/02/15 07:54 PM (8 years, 3 months ago)

Quote:

YaMoonSun said:
Should I cheat and buy ten of these for $17?
http://www.amazon.com/gp/product/B004MKHNJK?keywords=petri%20dishes&qid=1443758031




I've blown through 40 plates in a matter of 2 weeks and I am only on my second transfer! This is such a rip off. I think my biggest expense is the petri dishes and my time, but honestly, I much rather know that when a contaminant pops up that it was due to my fuck up than some pre-poured plates that I bought through amazon. But... I confess... I do love Amazon.


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Re: Petri Question [Re: Kalistis]
    #22325597 - 10/02/15 07:56 PM (8 years, 3 months ago)

You can get a case of 500 for around $80 shipped if you shop around.

That's the smart move.


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Re: Petri Question [Re: PussyFart]
    #22325647 - 10/02/15 08:06 PM (8 years, 3 months ago)

I just buy mine through a distributor at work. Perk! The price fluctuates depending on the manufacturer. I got lucky and got a case of 100 discontinued Falcons for free from a rep that was trying to bribe me into another deal.


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