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YaMoonSun
The Double Standard


Registered: 10/23/14
Posts: 3,967
Loc: NY
Last seen: 5 months, 11 days
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Petri Question
#22321326 - 10/01/15 09:32 PM (8 years, 3 months ago) |
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Is there a better alternative to parafilm, or is this information still legit? I want to get some petris very soon.
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YaMoonSun
The Double Standard


Registered: 10/23/14
Posts: 3,967
Loc: NY
Last seen: 5 months, 11 days
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It doesn't go over the vents, right? Should I put micropore tape over the vents? Do I need both?
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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You need vented. Just wrap with saran.
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YaMoonSun
The Double Standard


Registered: 10/23/14
Posts: 3,967
Loc: NY
Last seen: 5 months, 11 days
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Ummm no. Not only is that a ripoff, there is no guarantee how well those were prepared or if the agar was even properly sterilized. Do things yourself and be confident they were done right.
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YaMoonSun
The Double Standard


Registered: 10/23/14
Posts: 3,967
Loc: NY
Last seen: 5 months, 11 days
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They seem to have pretty decent reviews, but okay, you're more than likely right. I need to get a ton of supplies still then
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micro
bunbun has a gungun



Registered: 05/09/03
Posts: 7,532
Loc: Brick City
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Well, if it weren't sterile it would probably be culturing something by now, but it looks like LB media which is going to be preferential to bacteria anyway. I agree -- you're much better doing this yourself and using media that is specific to fungi, esp. basidiomycetes / dung lovers.
OP, if you are asking about parafilm, it is what they use in biological laboratories but we always threw the petris back in their sleeve and stored it in the walk-in fridge. I don't remember ever using that to culture them. Not sure if it is oxygen/co2 that is the reason; the dishes are often left upside-down and shut anyway, but I figured I'd mention it, for what it is worth.
(if I had to guess I'd say doesn't matter when culturing, but wrap it up to store)
-------------------- Any research paper or book for free (Avatar is Maxxy, a character by Mizzyam, RIP)
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YaMoonSun
The Double Standard


Registered: 10/23/14
Posts: 3,967
Loc: NY
Last seen: 5 months, 11 days
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Re: Petri Question [Re: micro]
#22321544 - 10/01/15 10:42 PM (8 years, 3 months ago) |
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It's likely because of co2 being heavier, it just falls out of the petri as after the mold/fungi produces it, thanks for the response. Good tip.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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co2 does not fall
--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Re: Petri Question [Re: cronicr]
#22322046 - 10/02/15 02:19 AM (8 years, 3 months ago) |
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U sure bro?!? I've seen it fall with my own 2 eyes son!
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YaMoonSun
The Double Standard


Registered: 10/23/14
Posts: 3,967
Loc: NY
Last seen: 5 months, 11 days
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Re: Petri Question [Re: cronicr]
#22323061 - 10/02/15 10:58 AM (8 years, 3 months ago) |
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Why wouldn't it if it's heavier than oxygen? Too much free air exchange mixing the co2 and oxygen up?
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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It's all these damn air pressures and currents! The world isn't a vacuum. Co2 and o2 would split if it was a vacuum.. Void of all air and currents.
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taGyo
Strainiac/AMU



Registered: 10/16/14
Posts: 18,802
Loc: Journal Land
Last seen: 5 years, 3 months
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CO2 and O2 mix together. It's not an oil and water kind of deal.
-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
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YaMoonSun
The Double Standard


Registered: 10/23/14
Posts: 3,967
Loc: NY
Last seen: 5 months, 11 days
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Re: Petri Question [Re: taGyo]
#22323249 - 10/02/15 11:43 AM (8 years, 3 months ago) |
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So those mycelium bags people put in their cannabis grow rooms to replace co2 tanks don't actually need to be elevated? Damn pot-heads. 
Just for the record, and I know it may sound stupid, but I don't cover the vents with the saran, only the sides of the petris, correct?
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aerow.thefox
professional amatuer



Registered: 06/10/10
Posts: 316
Loc: living w/dimensional elve...
Last seen: 3 years, 1 day
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Re: Petri Question [Re: taGyo]
#22323259 - 10/02/15 11:47 AM (8 years, 3 months ago) |
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Quote:
taGyo said: CO2 and O2 mix together. It's not an oil and water kind of deal.
im not sure im entirerly sure thats accurate. do they mix well and stay mixed in a normal enviroment? yea of course.
but they DO have different masses. and BOTH are gasses influenced by fluid dynamics. i would imagine if all gasses were trapped inside an air-tight container, with an even temperature throughout, the would naturally seperate into layers of gasses. with the most dense at bottom and least at top. without temperature or physical interaction causing disturbances in a material so easily moved... just my opinion.
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taGyo
Strainiac/AMU



Registered: 10/16/14
Posts: 18,802
Loc: Journal Land
Last seen: 5 years, 3 months
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Yeah, in a hypothetical world with hypothetical exacts they may or may not segregate themselves based on density and other variables. I haven't done enough research to tell you.
But in an SGFC, Petri or Mono the CO2 isn't simply on one level of the SGFC, it's mixed with the oxygen.
-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: Petri Question [Re: taGyo]
#22323567 - 10/02/15 12:46 PM (8 years, 3 months ago) |
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Not to mention heat currents being formed by the mycelium.
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aerow.thefox
professional amatuer



Registered: 06/10/10
Posts: 316
Loc: living w/dimensional elve...
Last seen: 3 years, 1 day
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exactly my point. in a normal enviroment they mix up just fine. too many variables. but they CAN seperate
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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CO2 can fall real fast real hard. I dropped a CO2 tank on my toe once and lost the nail
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tripdawg420
low life with no life



Registered: 02/02/09
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