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OfflineMysticMoteToter
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Is this a decent plan for a first grow?
    #22320529 - 10/01/15 06:54 PM (8 years, 3 months ago)

Hey everyone, I'm getting everything together for my first try at PF cakes but before starting i want to see if anyone sees any flaws in my plan.

      I'm planning on making MEA Petris and using an inoculation loop and a spore print to colonize some petris.

      Once i have a clean and decent myc. culture in the petris, i was planning on dropping a chunk of the colonized agar into a honey-water LC.

      I'd then let the mycelium colonize the LC, once it is ready I'd use the LC to make a syringe and inoculate PF cakes from there.

      The transfers and inoculations would be done in a SAB.

      Is there anything key i'm forgetting or a reason why this wont work, any extra things i should take note of before?

      Thanks everyone and i look forward to the feedback.


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InvisibleInocuole
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Re: Is this a decent plan for a first grow? [Re: MysticMoteToter]
    #22320550 - 10/01/15 06:57 PM (8 years, 3 months ago)

That uh.... that would blow most people's first grows out of the water if you did everything right.  Good luck with that.


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OfflineiSmkGrnBud
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Re: Is this a decent plan for a first grow? [Re: Inocuole]
    #22320587 - 10/01/15 07:04 PM (8 years, 3 months ago)

:whathesaid:

Skip the LC. If you must just make up some quick LI for your PF tek. Personally I would go straight from agar. Good luck. :cheers:


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InvisibleMunchauzen
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Re: Is this a decent plan for a first grow? [Re: Inocuole]
    #22320594 - 10/01/15 07:06 PM (8 years, 3 months ago)

I'd replace the LC with LI, but thats just personal preference.

http://www.shroomery.org/forums/showflat.php/Number/20429745


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OfflineMysticMoteToter
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Re: Is this a decent plan for a first grow? [Re: Inocuole]
    #22320602 - 10/01/15 07:08 PM (8 years, 3 months ago)

Thats kinda what i mean its probably seeming easier in my head. Is there any real chance of me being successful is kinda what i mean haha. The reason i was planning to do it this way is b/c i cant get spore syringes where i'm at and this seemed like the best way to prevent contams, (b/c the print i'm getting is likely to be a bit contaminated), its free from FSRE so i don't care and i got agar for the purpose, but i'm worried about just using the spores in a syringe without knowing if there is contaminates. Do you think it'd be a better idea just to use the spores and make a syringe and then use just spores to knock up some PF's? I just really don't want to waste my spores and time making a spore syringe only to find a contaminated cake later


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Re: Is this a decent plan for a first grow? [Re: MysticMoteToter]
    #22320609 - 10/01/15 07:09 PM (8 years, 3 months ago)

This is the best chance of being successful, I'd say.  Better than spores, especially if you do a LI.


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Re: Is this a decent plan for a first grow? [Re: Inocuole]
    #22320622 - 10/01/15 07:12 PM (8 years, 3 months ago)

I need to do some reading up on LI's. Is there a favorite LI tek anyone prefers?


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Re: Is this a decent plan for a first grow? [Re: Munchauzen]
    #22320625 - 10/01/15 07:12 PM (8 years, 3 months ago)

Quote:

Munchauzen said:
I'd replace the LC with LI, but thats just personal preference.

http://www.shroomery.org/forums/showflat.php/Number/20429745




--------------------
                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

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Re: Is this a decent plan for a first grow? [Re: Inocuole]
    #22320647 - 10/01/15 07:16 PM (8 years, 3 months ago)

Thank you, i dont know how i missed that, my bad  :doublefacepalm: Thanks again everyone.


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