Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
InvisibleRenegadeBlue
Gourmet
I'm a teapot

Registered: 06/08/15
Posts: 222
Loc: USA
Questions About My Failed Agar Attempt *DELETED*
    #22319986 - 10/01/15 04:43 PM (8 years, 3 months ago)

Post deleted by RenegadeBlue

Reason for deletion: .



Extras: Filter Print Post Top
OfflinePsilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
Re: Questions About My Failed Agar Attempt [Re: RenegadeBlue]
    #22320034 - 10/01/15 04:57 PM (8 years, 3 months ago)

I had the same condensation problem with my pasty plates. Make sure to empty out any residual moisture that may have dropped down onto the agar(in a SAB of course). When the surface of the agar is relatively dry, then take your clone. Make sure to take from the very inside of the fruit. You only need a small piece.

When working inside your SAB, make sure not to let your hand hover over the plate and always flame sterilize, good sterile techniques are the most important aspect of having clean growth on your plates.


Extras: Filter Print Post Top
OfflineGreenRabbit
Plutonium Pollinator
Male


Registered: 04/28/13
Posts: 2,667
Loc: In a forest
Last seen: 2 years, 3 months
Re: Questions About My Failed Agar Attempt [Re: RenegadeBlue]
    #22320036 - 10/01/15 04:58 PM (8 years, 3 months ago)

Too much condensation can create little pools like that. It looks like you lots of bacteria (clear bubbly area) and I have no idea what that white stuff is.
No good though.

Are you just trying to clone here?


Extras: Filter Print Post Top
Invisibleblackdust
 User Gallery

Registered: 02/28/09
Posts: 8,327
Re: Questions About My Failed Agar Attempt [Re: GreenRabbit]
    #22320091 - 10/01/15 05:16 PM (8 years, 3 months ago)

use glass


Extras: Filter Print Post Top
OfflineSillyPsy
Strangest

Registered: 08/18/15
Posts: 52
Last seen: 7 years, 9 months
Re: Questions About My Failed Agar Attempt [Re: GreenRabbit]
    #22320102 - 10/01/15 05:18 PM (8 years, 3 months ago)

I do the no pour agar myself. A few things I've found is that first,
I let the agar cool before putting the lids on,before PC'ing. Which only takes a few minutes,if that.
I also put a paper towel between the lid and foil, which I remove after I PC. Then I "tent" the foil, to let some airflow in, but still cover the top. I usually just leave them like that in my SAB with the arm holes open.
Letting them cool in the PC before removing seemed to help a little. Just have to make sure they're level, or your agar will pool to one side. I only use about 1-2 Tbsp of agar mix, just enough to cover the ridge on the bottom of the cup.
Finally, I give them about 4-5 days at room temp, and most of the moisture seems to disappear. If it doesn't I lightly tap them on the table, to consolidate it into a few drops. I'll either wait till that disappears, or when I'm in the SAB I'll tip it over and remove the excess onto a paper towel before doing the transfer.

Honestly though, the most I've had to wait is a week before there's 0 moisture/condensation left. Not sure if it's evaporating, or being absorbed, or both. But I can say, with proper sterile procedure and dry dishes I've had almost no contams.

I tried it with the condensation like that once, with almost the same results as you have.


Extras: Filter Print Post Top
Jump to top Pages: 1


Similar ThreadsPosterViewsRepliesLast post
* Questions on first attempt Voyager 823 3 12/19/02 07:29 AM
by upupup
* question on agar Ripple 2,054 10 09/19/02 04:40 PM
by Ripple
* some questions about agar ar393 996 3 06/11/01 03:04 PM
by Anno
* Question about agar supplies Chemical_Smile 1,545 3 04/20/02 05:50 AM
by babyshroom
* Innoculating with agar wedge observations homebrew 3,505 4 03/01/02 08:00 AM
by homebrew
* Agar Questions cantara 5,200 6 08/09/01 06:22 AM
by ar393
* MS to grain VS. MS to agar? should the germination time be similar or no? more4u2c 690 9 03/22/18 02:49 AM
by more4u2c
* Question about strains
( 1 2 all )
GaNjAShRooM 3,220 32 03/06/02 06:07 AM
by GaNjAShRooM

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
259 topic views. 35 members, 257 guests and 48 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.025 seconds spending 0.009 seconds on 14 queries.