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Invisiblemicro
bunbun has a gungun
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Registered: 05/09/03
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Some references for media for isolation of bacidiomycetes...
    #22317981 - 10/01/15 08:59 AM (8 years, 3 months ago)

Since it was asked here, and I just ran by this:

(edit: obviously this is for woodlovers; adjust accordingly)

http://www.researchgate.net/profile/Ed_De_Jong2/publication/40184757_Isolation_and_screening_of_basidiomycetes_with_high_peroxidase_activity._Mycol_Res/links/0fcfd50185c7c469fc000000.pdf?inViewer=true&&origin=publication_detail&inViewer=true

Quote:

A modified method of Nishida et al. (1988) was used for isolation of basidiomycetes. Pieces of rotted wood (about 1 x 0'5 x 0'5 em) or a small amount of soil (about 1 g) were placed in the centre of plates containing hemp stem wood (0'2 %), guaiacol (0'01 %), benomyl (Du Pont Chemical Co., Wilmington, U.S.A., a 50% wettable powder) (15 ppm), agar (1'5%) and sometimes also chloramphenicol (200 ppm). Plates were incubated at either 28, 30 or 45°, and subcultures made as soon as browning of agar occurred (indicative of ligninolytic activity, Nishida et al., 1988) until the isolates were pure.




Rotting wood: I'm sure you can find this :V
Hemp stem wood: I'm sure you can find this, too :rolleyes:
Guaiacol: This is used to make eugenol and vanillin and should be sold by aroma chemical companies
Benomyl: This is a fungicide regularly sold in places like WalMart or Home Depot
Agar: This should be in the FAQ
Chloramphenicol: You might have a hard time finding this, but it is optional and could be replaced with another bacteriostatic, anyway

Quote:

isolated strains were maintained at 4 °c on agar plates containing BIll medium (Kirk et al., 1986) with (0'2 %) hemp (Cannabis sativa L., grown in The Netherlands in 1987) stem wood fibres and (0'02%) Poly R-478 (Sigma, St Louis, U.S.A.). Poly R-478 was
included to confirm that isolates still contained Poly R decolorizing activity.




(ignore the part about poly r)

Quote:

In all experiments, serum bottles were inoculated with cylindrical agar plugs (6 mm diameter), all taken 3 em from the initial inoculum, containing the following sterile media: (1) Hemp stem wood (HSW) medium, containing 2 g 1-1 IOD-IOOO ~m classified particles of hemp stem wood in 10 Il1M sodium 2,2-dimethylsuccinate (DMS), at pH
4'5; (2) BIll medium, prepared according to Kirk et al. (1986); (3) Kirk medium, containing glucose (1 % w Iv), DMS (10 mM), BIll, NH 4 tartrate (0'2 g 1-1) and thiamin (2 mg 1-1) according to Tien & Kirk (1988), veratryl alcohol and extra trace elements being omitted. All components were filter sterilized unless stated otherwise. Incubation was at 30° except with P. chrysosporium, which was grown at 37°.




2,2-dimethylsuccinate: I'm guessing this is just the methyl ester of succinic acid. I can't find it but you can take succinic acid and add methanol and concentrated H2SO4 and distil as the reaction is going.
ammonium tartarate: This seems pretty common and available.
thiamin: Vitamin B-1

http://www.nature.com/nature/journal/v177/n4518/pdf/1771038b0.pdf

http://www.sciencedirect.com/science/article/pii/026530368990016X

Quote:

Isolations from mycelial fans were obtained by removing bark and cambial tissue from decayed wood samples, after which small pieces of the underlying mycelial fans were plated directly onto agar. Isolates were grown on malt extract agar (15 mg/mL malt extract, 15 mg/mL agar) (Maloy 1974; Rizzo and Harrington 1988) amended with 10 mL/L benomyl (added as a stock solution by dissolving 40 mg Benlate 50% WP in 50 mL of warm ethanol, diluting to 100 mL with distilled water (Worrall 1991)) and 100 mg/L of streptomycin sulfate in distilled deionized water (streptomycin added after autoclaving). After 4 to 5 weeks of growth in darkness at 24°C, rhizomorphs or mycelia were harvested and dried for approximately 24 h on filter paper in a laminar flow hood.




http://www.researchgate.net/publication/245584017_Benomyl-malt_agar_for_the_purification_of_cultures_of_wood_decay_fungi



I found this rather amusing:

Quote:

Purpose (Fruiting Structure Induction Media)

Excellent for the sporulation of various fungi, but especially for Thielaviopsis basicola.

Ingredients
Ingredient 1 L 500 mL
distilled water 800 mL 400 mL
V8 juice 200 mL 100 mL
CaCO3 2 g 1 g
agar 15 g 7.5 g


Instructions

Mix ingredients
Autoclave and cool to 50-55°C

Notes

  • Recipes for this medium vary with respect to the amount of V8 juice added. Some use one 6 oz. can (180 ml/liter) because it is convenient.
  • The calcium carbonate is reported to prevent the acidity of the juice from hydrolyzing the agar.
  • Rob Wick reports no problems with solidification when CaCO3 was lacking.
  • This medium is often used to grow Pythium and Phytophthora.
  • If it is important to view microscopic features directly on the medium, it should be clarified by filtration or centrifugation before sterilization.


References

Dhingra, O. D., and J. B. Sinclair. 1985. Basic Plant Pathology Methods. CRC Press, Inc., Boca Raton, FL. 355 pp.

Stevens, R. B. 1974. Mycology Guidebook. University of Washington Press, Seattle, WA. 703 pp.




http://wiki.bugwood.org/V8_agar

Actually here are a whole bunch in that category:

http://wiki.bugwood.org/Category:Fruiting_Structure_Induction_Media


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Edited by micro (10/01/15 03:18 PM)


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