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Invisibleeatyualive
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Re: taping holes in monotubs/casing at spawning (moved) [Re: bodhisatta]
    #22835829 - 01/28/16 08:05 PM (8 years, 2 days ago)

hell of a flush dhearic! :rockon:


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InvisiblePastywhyteMDiscord
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Re: taping holes in monotubs/casing at spawning (moved) [Re: RJ Tubs 202]
    #22835831 - 01/28/16 08:06 PM (8 years, 2 days ago)

Quote:

RJ Tubs 202 said:
Quote:

Pastywhyte said:
I'm not recommending anything. I'm saying that mold doesn't germinate on coir. Sure other materials may be present in the coir but at that point it's not the coir persay that contamed but rather the offending material.

Also trichoderma has a white mycelium and will not go immediately green. Molds have been shown to often be meshed in and very difficult to distinguish in spawn.




I'm confused. You believe Trichoderma is or isn't sometimes present in coir? 

Yes, the seed became contaminated, I agree. The issues is when and how. I fruited a couple of containers without spawning to coir (Violet's tek) and they were fine.

Trichoderma can be very trichy to spot. I use RGS with well ventilated containers and molds sporulate fast and my seed turns green quickly if the seed is contaminated.

All just one dudes opinion of course. :laugh:




I'm saying that whether trich is present in the coir or not is moot. If it's there it could be a vector if something was preventing your grain from recovering (like bacteria). But given how much mold is already floating around, its there no matter what you spawn to. Healthy clean spawn is going to recover and any mold spores that may have come into contact with it will have no food source to germinate with. Instead they become the food :lol:

Trich is not added to pet grade coir FYI. Reptiles actually have pretty sensitive resperatory systems so adding something like spores to a product meant to be used as bedding for them would be a really bad idea. They add it to hydro grade coir because it's beneficial for plants.

Also there are many molds that have green spores that are not trich and will sporulate invitro.


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Invisibleeatyualive
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Re: taping holes in monotubs/casing at spawning (moved) [Re: bodhisatta]
    #22835837 - 01/28/16 08:07 PM (8 years, 2 days ago)

Quote:

besides you are going to either bucket tek, pasteruize, or sterilize the coir anyway.





NOT ANYMORE

:uptosomething:

what is pasteruize?


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Offlinebollarice
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Re: taping holes in monotubs/casing at spawning (moved) [Re: eatyualive]
    #22836551 - 01/28/16 10:36 PM (8 years, 2 days ago)

Quote:

PussyFart said:
He places the handles of the scalpels in the PC when he sterilized his agar, so they get sterilized as well.




oh, got it, duh. I thought somehow agar was part of some sterilization process. And I was like
:mindblown:


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OfflineDhearic
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Re: taping holes in monotubs/casing at spawning (moved) [Re: eatyualive]
    #22837038 - 01/29/16 01:06 AM (8 years, 2 days ago)

Quote:

eatyualive said:
hell of a flush dhearic! :rockon:




:hatsoff:

Thanks my man!! They are small but many

I put that stro on some agar getting ready to go to some cornmeal plates with here soon.

I've got a need for speed :lol:


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Credit where credit is due.


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OfflineRJ Tubs 202
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Re: taping holes in monotubs/casing at spawning (moved) [Re: Pastywhyte]
    #22840877 - 01/30/16 12:36 AM (8 years, 1 day ago)

Quote:

Pastywhyte said:

I'm saying that whether trich is present in the coir or not is moot.





OK, now I get the idea.

That said, do you think pasteurization of peat based casing is unnecessary?

For those of us who have not evolved to the higher level of not casing . . .


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Invisibleblindingleaf
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Re: taping holes in monotubs/casing at spawning (moved) [Re: RJ Tubs 202]
    #22841060 - 01/30/16 02:52 AM (8 years, 1 day ago)

technically it is not necessary, but IMO, it is a good idea for two reasons.

1. tubs don't necessarily have the best FAE (like a GH or open air grow) so if there are a lotos mold spores present in the peat, a mono tubs environment would be a good place for them to grow.
2. pasteurization kills gnat/fly eggs that may be in the peat


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MICROBIAL HUSBANDRY!!!!

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InvisibleMr.PhilCybin
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Re: taping holes in monotubs/casing at spawning (moved) [Re: blindingleaf]
    #22850107 - 02/01/16 11:50 AM (7 years, 11 months ago)

I guess it's not 100% relevant since it's its not a mono, but I'm doing a very low tech experiment with this on some mini trays in the gh.

1 quart rye was spawned to coir/straw/verm/gypsum in somewhere around a 1:1 ratio.
6 trays total. 3 went straight into the gh, and 3 were covered to colonize first.

curious how it'll turn out and what yield will be per qt compared to a mono.
strain is pesa.
I was gonna do another 6 the same way and case them, but ran out of sub so I went into a deeper container. but that's not relevant at all here.
ill update when something happens.



--------------------
I'm stupid, Falcon91Wolvrn03 is smart.
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I'm a loser, Falcon91Wolvrn03 is a winner.
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Invisibleazur
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Re: taping holes in monotubs/casing at spawning (moved) [Re: Mr.PhilCybin]
    #22850218 - 02/01/16 12:24 PM (7 years, 11 months ago)

:pullingupachair:


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A cube is NOT a cube.

FALL IN LOVE WITH LC
FOTTSE!!!
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Invisibleazur
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Re: taping holes in monotubs/casing at spawning (moved) [Re: PussyFart] * 1
    #22855997 - 02/02/16 05:08 PM (7 years, 11 months ago)

Quote:

PussyFart said:
I have never showered before doing any sterile work and rarely have issues....

If you were working in a SAB and following proper sterile procedures I doubt it had anything to do with it, but this is just my opinion.



Ya know, i let this go before, but i shouldn't have.
You can bang girls without rubbers and drive cars without seatbelts, but it's still bad practice.
Advocating not showering before doing sterile work is just looking for an argument. Had you read a real mycologist's book (stamets) and not watched some corny videos by rr, you'd know you should shower before hand.
Yes, you can get away with it. But it's not the best or even the proper way.  So you of all people  (a trusted cuntivator) shouldn't be giving out shitty advice.


--------------------


A cube is NOT a cube.

FALL IN LOVE WITH LC
FOTTSE!!!
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Invisiblethe_r3dz
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Re: taping holes in monotubs/casing at spawning (moved) [Re: azur]
    #22856143 - 02/02/16 05:44 PM (7 years, 11 months ago)

It's funny because after reading this I started becoming a little bit lax on my procedure, I used to shower every time I had something I had to do in my box, lately I've just been slipping my gloves on (got these awesome elbow length plastic gloves) and going in.

Had a bunch of plates contam :facepalm: lesson learned.


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OfflinePussyFart
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Re: taping holes in monotubs/casing at spawning (moved) [Re: azur] * 1
    #22856149 - 02/02/16 05:45 PM (7 years, 11 months ago)

Quote:

azur said:
Quote:

PussyFart said:
I have never showered before doing any sterile work and rarely have issues....

If you were working in a SAB and following proper sterile procedures I doubt it had anything to do with it, but this is just my opinion.



Ya know, i let this go before, but i shouldn't have.
You can bang girls without rubbers and drive cars without seatbelts, but it's still bad practice.



Your comparison makes no sense.....I am not using my dick in my sab, but I do used gloved hands/arms......and seat belts are there "just in case"your vehicle collides with something else....what can happen in a SAB from me not washing my balls before a transfer??? My balls are nowhere near the sab.

Quote:

azur said:
Advocating not showering before doing sterile work is just looking for an argument.



So your balls go inside your SAB?

Quote:

azur said:
Yes, you can get away with it. But it's not the best or even the proper way.  So you of all people  (a trusted cuntivator) shouldn't be giving out shitty advice.



Give me one good example of how not washing my balls can lead to my sterile procedure going haywire, when it's all done in a totally separate room than my balls....or my feet, or my chest.....

None of that stuff matters if I am working in a SAB.....the dead skin cells and/or mold spore from my foot are not going to magically find their way into my SAB.

Now if we are talking about my hands/arms, that is different....they get washed/covered.

Plus I am not advocating anything, I was simply stating facts about my procedures.

I think RR is just trying to tell us we stink.


Edited by PussyFart (02/02/16 05:47 PM)


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Invisibleazur
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Re: taping holes in monotubs/casing at spawning (moved) [Re: PussyFart]
    #22856328 - 02/02/16 06:20 PM (7 years, 11 months ago)

I wasn't trying to be combative. I just want noobs to cover all the steps.
And i never said anything about you tiny balls. But it is a good idea to wash from the waist up, including your hair.
Again, i know a lot of people don't shower before working. Hell, i don't even think eatyualive does. But you can't deny that it's not a bad idea


--------------------


A cube is NOT a cube.

FALL IN LOVE WITH LC
FOTTSE!!!
ALL NOOBS READ THIS!!!



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InvisibleMr.PhilCybin
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Re: taping holes in monotubs/casing at spawning (moved) [Re: azur]
    #22856348 - 02/02/16 06:24 PM (7 years, 11 months ago)

i completely agree that you shouldn't encourage it, but I get away with not showering, and I stopped used gloves a couple months ago when I ran out, and I probably only have about a >10% contam rate on my plates.  I work with a flowhood, so it's a little easier (IMO) to be clean than a SAB, but I'd never advocate it.  it's just lazy :shrug:

i usually try to shower and brush my teeth, but I won't make a big deal about it.
i run the hood in my room for like 30 min prior to working, and spray the crap out of my hands with alcohol while i work and i get by fine.

with all that said, i need to get gloves again, cause why not, but its not 100% mandatory unless you're running a commercial op and time is of the essence


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I'm a loser, Falcon91Wolvrn03 is a winner.
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InvisiblebodhisattaMDiscordReddit
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Re: taping holes in monotubs/casing at spawning (moved) [Re: Mr.PhilCybin] * 1
    #22856371 - 02/02/16 06:27 PM (7 years, 11 months ago)

you absolutely positively do not need to take a shower or brush your teeth before doing sterile work, because if you're good at sterile work you're not a vector in the first place.

they don't make students at the medical college take a shower before working in any lab that I'm aware of. and most of them are better than us at sterile work.


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InvisibleMr.PhilCybin
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Re: taping holes in monotubs/casing at spawning (moved) [Re: bodhisatta]
    #22856380 - 02/02/16 06:29 PM (7 years, 11 months ago)

Quote:

bodhisatta said:
and most of them are better than us at sterile work.



:justno:

im the best mycologist in the lab bruh


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I'm stupid, Falcon91Wolvrn03 is smart.
I'm ugly, Falcon91Wolvrn03 is beautiful.
I'm a loser, Falcon91Wolvrn03 is a winner.
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InvisiblebodhisattaMDiscordReddit
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Re: taping holes in monotubs/casing at spawning (moved) [Re: Mr.PhilCybin]
    #22856400 - 02/02/16 06:32 PM (7 years, 11 months ago)

well when you work in a lab that deals with pathogens like TB you have an instructor standing behind you the whole time, never been in that position but it looks pretty nerve-wrecking.


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InvisibleMr.PhilCybin
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Re: taping holes in monotubs/casing at spawning (moved) [Re: bodhisatta]
    #22856413 - 02/02/16 06:35 PM (7 years, 11 months ago)

:lol: yeah i was obviously kidding.

im sure working with something that has greater consequences than just trich'ing out would get you pretty good at sterile work quick.

luckily for me things are low consequence and i like it that way


--------------------
I'm stupid, Falcon91Wolvrn03 is smart.
I'm ugly, Falcon91Wolvrn03 is beautiful.
I'm a loser, Falcon91Wolvrn03 is a winner.
Someday, I hope to be like Falcon91Wolvrn03 but secretly know I never will.


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Invisibleazur
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Re: taping holes in monotubs/casing at spawning (moved) [Re: bodhisatta]
    #22856425 - 02/02/16 06:38 PM (7 years, 11 months ago)

In that type of lab setting, it's completely different. The flow of the hood is opposite.
Look, there's no argument. I already said you don't have to shower. But you should.  And to tell noobs not to is doing them a big injustice. Shame on anyone giving out bad info for the sake of a good argument


--------------------


A cube is NOT a cube.

FALL IN LOVE WITH LC
FOTTSE!!!
ALL NOOBS READ THIS!!!



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InvisiblebodhisattaMDiscordReddit
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Re: taping holes in monotubs/casing at spawning (moved) [Re: azur]
    #22856453 - 02/02/16 06:44 PM (7 years, 11 months ago)

it's not bad info, it's info you don't like because you want to ride PFs nuts.

if it was bad info 99% of us would be taking showers because we wouldn't get good results being un-showered.

shame on you for trying to be 100% right on a place where different opinions and techniques work, and no one has the right answer. 100% proof that showering is needless is the results everyone has though.

and the flow isn't opposite it's either a vertical or horizontal flow hood the air comes from the top down or the back forward.


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