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Machiavelliavore
Vermiculite Hater



Registered: 12/08/14
Posts: 3,038
Loc: The Sporetorn States
Last seen: 3 months, 19 days
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Re: taping holes in monotubs/casing at spawning (moved) [Re: PussyFart]
#22830929 - 01/27/16 03:28 PM (8 years, 3 days ago) |
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Quote:
PussyFart said: I cover my hands with gloves, and arms with tyvek...arms and hands get washed beforehand...these are the only parts of my body that go inside my SAB.....why would the rest of my body matter if the air in the room I work in is still?
This has always been my philosophy. I could see a clean shirt potentially being helpful. At least not wearing a shirt that I've thrown out contamed subs in.
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I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister. I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave. I think it will be good cause B+ is so potent. Triggered yet? Only a square would say "a cube is a cube."
No, this does not look right...
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: taping holes in monotubs/casing at spawning (moved) [Re: Machiavelliavore]
#22831330 - 01/27/16 05:20 PM (8 years, 3 days ago) |
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DrEarnhardt



Registered: 10/21/15
Posts: 150
Last seen: 8 months, 28 days
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Re: taping holes in monotubs/casing at spawning (moved) [Re: eatyualive]
#22832115 - 01/27/16 09:09 PM (8 years, 3 days ago) |
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Today is day 5 on this tub. I've misted a couple times since I spawned. I kind of expected them to start pinning by now. How does it look? Should I be changing something? Remember this is my first mono.


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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: taping holes in monotubs/casing at spawning (moved) [Re: DrEarnhardt]
#22832149 - 01/27/16 09:18 PM (8 years, 3 days ago) |
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your almost there. it looks good. i see the beginning of knotting. 1-2 more days you will see pins.
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DrEarnhardt



Registered: 10/21/15
Posts: 150
Last seen: 8 months, 28 days
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Re: taping holes in monotubs/casing at spawning (moved) [Re: eatyualive]
#22832381 - 01/27/16 10:22 PM (8 years, 3 days ago) |
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Awesome. Thanks Eat!
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: taping holes in monotubs/casing at spawning (moved) [Re: DrEarnhardt]
#22833028 - 01/28/16 05:54 AM (8 years, 3 days ago) |
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your welcome! can't wait to see your results.
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Dhearic


Registered: 01/24/14
Posts: 846
Loc: Neverland
Last seen: 9 months, 3 days
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Re: taping holes in monotubs/casing at spawning (moved) [Re: eatyualive]
#22833290 - 01/28/16 07:55 AM (8 years, 3 days ago) |
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, 
Kinda disappointed with the size of the fruits but that's what you get with MS 
All in all I'd say I'm quite pleased. A little over a month ago I had a fully colonized plate of Wikidzon and now I have 3 tubs fruiting. Speed rocks!
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Credit where credit is due.
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bollarice
Subtle, and quick to anger


Registered: 03/19/14
Posts: 728
Loc: twoston
Last seen: 2 months, 20 days
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Re: taping holes in monotubs/casing at spawning (moved) [Re: azur]
#22834959 - 01/28/16 04:50 PM (8 years, 2 days ago) |
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This might be a dumb question, but...
Quote:
azur said: I also like to sterilize my scalpels with agar so the handles are sterile
..wait, what? Can you unpack what you mean by sterilizing the handle with agar?
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I've been workin' in the Kremlin with a two-headed dog.
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 17 days, 18 hours
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Re: taping holes in monotubs/casing at spawning (moved) [Re: bollarice]
#22834967 - 01/28/16 04:52 PM (8 years, 2 days ago) |
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He places the handles of the scalpels in the PC when he sterilized his agar, so they get sterilized as well.
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THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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RJ Tubs 202



Registered: 09/20/08
Posts: 6,016
Loc: USA
Last seen: 6 hours, 55 minutes
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Re: taping holes in monotubs/casing at spawning (moved) [Re: Pastywhyte]
#22835195 - 01/28/16 05:47 PM (8 years, 2 days ago) |
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Quote:
Pastywhyte said: Getting green with coir 95% of the time points to the spawn. I would however be hesitant to use hydroshop coir untreated. Pet grade sure but not for coir bought in a hydro shop.
I recently got some "Zoo Med Eco Earth" coir from the Petco. I didn't treat it, and spawned 7 different containers of RGS with it, creating 7 mini-trays, and all 7 trays got Trichoderma.
Then I threw the same coir into hot water, and did the same thing, this time with 9 containers of spawn creating 9 min-trays, and they were all clean and fruited.
I've had this happen before, and although you might sometimes get away with no treatment, I will always treat coir in the future.
It's a tiny investment in time/effort, and why risk it when you are on the one yard line. I cry rivers of tears when I ruin beautiful clean spawn.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: taping holes in monotubs/casing at spawning (moved) [Re: RJ Tubs 202] 1
#22835257 - 01/28/16 05:58 PM (8 years, 2 days ago) |
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I take that same pet shop coir and put it unheated and soaked in my snakes hide box for him. It doesn't mold even with him pissing and shitting in it. It wasn't the coir. It's meant to be soaked down unheated and left. That's the reason reptile enthusiasts use it in the first place.
Your beautiful clean spawn was contamed.
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RJ Tubs 202



Registered: 09/20/08
Posts: 6,016
Loc: USA
Last seen: 6 hours, 55 minutes
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Re: taping holes in monotubs/casing at spawning (moved) [Re: Pastywhyte]
#22835520 - 01/28/16 06:49 PM (8 years, 2 days ago) |
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Quote:
Pastywhyte said:
Your beautiful clean spawn was contamed.
Just because wet coir doesn't grow Trichoderma in your snake box doesn't mean the coir doesn't contain Trichoderma. Same with, for example, wood shavings, straw, walnut shells, etc. My straw hat frequently gets wet and no contaminants grow on it (of course it's loaded with contams). Just because contaminants don't grow on something doesn't mean contaminates aren't present.
When you crumble grain or seed spawn, the grains and seeds are exposed, and vulnerable to contamination. Do you agree with that?
Trichoderma is super easy to see on RGS as the seed turns green immediately. My spawn was clean. The hypothesis 7 spawn containers were contaminated and then, from the same plate and PC run, 9 containers were clean, the only difference being heat treated coir, defies my sense of probability, and would be akin to winning the Powerball loto.
If people have success not treating coir, great. It's just so easy to do I wouldn't recommend anyone new to the hobby skip the step.
Edited by RJ Tubs 202 (01/28/16 07:01 PM)
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 17 days, 18 hours
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Re: taping holes in monotubs/casing at spawning (moved) [Re: RJ Tubs 202]
#22835535 - 01/28/16 06:53 PM (8 years, 2 days ago) |
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I think you misquoted Pasty....lol
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THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: taping holes in monotubs/casing at spawning (moved) [Re: PussyFart]
#22835592 - 01/28/16 07:08 PM (8 years, 2 days ago) |
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the trich they add to coir is harmless anyway, the only way that trich is going to germinate is if you mix it with soil and add a plant.
besides you are going to either bucket tek, pasteruize, or sterilize the coir anyway.
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RJ Tubs 202



Registered: 09/20/08
Posts: 6,016
Loc: USA
Last seen: 6 hours, 55 minutes
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Re: taping holes in monotubs/casing at spawning (moved) [Re: PussyFart]
#22835611 - 01/28/16 07:12 PM (8 years, 2 days ago) |
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Thank you for noticing my gaff PF... It's been corrected 
"PussyFart" was in my text box because I started to reply to your post.
Quote:
PussyFart said: I have never showered before doing any sterile work and rarely have issues....
If you were working in a SAB and following proper sterile procedures I doubt it had anything to do with it, but this is just my opinion.
I work in a greenhouse almost every day (doing entomology and pathology research) with clouds of potting soil dust from the soil mixer, and I come home covered in Trichoderma. I only wash my hands and arms when using my SAB and am proud of my microscopic contamination rate. I worked in tissue culture labs for 15 years, in hoods every day, and am amazed how well a SAB works. When I moved into a small apartment during a separation, I gave my Fungi Perfecti hood to a buddy, and use a SAB now. They rock.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: taping holes in monotubs/casing at spawning (moved) [Re: RJ Tubs 202]
#22835652 - 01/28/16 07:23 PM (8 years, 2 days ago) |
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I'm not recommending anything. I'm saying that mold doesn't germinate on coir. Sure other materials may be present in the coir but at that point it's not the coir persay that contamed but rather the offending material.
Also trichoderma has a white mycelium and will not go immediately green. Molds have been shown to often be meshed in and very difficult to distinguish in spawn.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: taping holes in monotubs/casing at spawning (moved) [Re: Pastywhyte]
#22835698 - 01/28/16 07:34 PM (8 years, 2 days ago) |
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nah dawg I got microscope eyes
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: taping holes in monotubs/casing at spawning (moved) [Re: bodhisatta]
#22835704 - 01/28/16 07:35 PM (8 years, 2 days ago) |
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Go go gadget
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RJ Tubs 202



Registered: 09/20/08
Posts: 6,016
Loc: USA
Last seen: 6 hours, 55 minutes
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Re: taping holes in monotubs/casing at spawning (moved) [Re: Pastywhyte]
#22835755 - 01/28/16 07:48 PM (8 years, 2 days ago) |
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Quote:
Pastywhyte said: I'm not recommending anything. I'm saying that mold doesn't germinate on coir. Sure other materials may be present in the coir but at that point it's not the coir persay that contamed but rather the offending material.
Also trichoderma has a white mycelium and will not go immediately green. Molds have been shown to often be meshed in and very difficult to distinguish in spawn.
I'm confused. You believe Trichoderma is or isn't sometimes present in coir?
Yes, the seed became contaminated, I agree. The issues is when and how. I fruited a couple of containers without spawning to coir (Violet's tek) and they were fine.
Trichoderma can be very trichy to spot. I use RGS with well ventilated containers and molds sporulate fast and my seed turns green quickly if the seed is contaminated.
All just one dudes opinion of course.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: taping holes in monotubs/casing at spawning (moved) [Re: RJ Tubs 202]
#22835808 - 01/28/16 08:00 PM (8 years, 2 days ago) |
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Quote:
You believe Trichoderma is or isn't sometimes present in coir?
some coir is treated with trichodermia spores. A lot of plants need a symbiotic relationship with molds like trich and other soil microbes. The trich is added to make the coir better suited for weed and tomatoes. but trich spores are easily killed even by the low end of pasteurization temperatures. the bucket tek is beyond pasteurization as it raises the temperature above 170F for more than 10m it achieves some level of "sterilization" defined by the killing off of any vegetative growth, while spores are spared. pasteurization for our mycology purpose leaves beneficial bacteria alive. seeing as there is none in coir. even if you pasteurize it all you're really doing is cooking it. if you're just cooking it you might as well forget trying to monitor temperatures and just fucking cook it. You can only pasteurize things like straw, and manure, substrates with additives like coffee and worm castings. those are the ones that need you to keep between 140-160F for an hour. simply going above 140F for long enough kills trich spores dead anyway.
though if you use enough clean spawn you could get away with using coir simply hydrated with cold tap water. mycelium prefers cooked coir though, the blacker the better, turn it back into soil with that pressure cooker.
 stove fuck that coir back to hell!
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