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OfflineRo0k
Stranger

Registered: 08/29/15
Posts: 95
Last seen: 6 years, 3 months
Good news/bad news time....
    #22315665 - 09/30/15 06:41 PM (8 years, 3 months ago)

Good news.  After almost 3 weeks in the incubator I found this this morning!



Bad news.... I also found this:




Of the 5 jars I had, 3 had various forms of green/black mold in 'em, and 2 have nothing showing yet.  Those 3 are sitting in the PC as we speak, to be dumped outside and subsequently bleached. 

This makes 13 jars I've lost to contams in the last month and a half - two grows with zero results.  Good news is, every time I try, it gets a little better.  Definitely need to work on my sterile procedures. 

On the bright side, it's teaching me that the GT strain I'm working with takes a LOOONG time to bloom from spores.  So patience, patience, patience.


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Invisiblenatedawgnow
Rocky mountain hood rat
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Registered: 02/09/15
Posts: 8,939
Loc: ation
Trusted Cultivator
Re: Good news/bad news time.... [Re: Ro0k]
    #22315952 - 09/30/15 07:37 PM (8 years, 3 months ago)

Sounds like it could also be a dirty syringe.


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OfflineRo0k
Stranger

Registered: 08/29/15
Posts: 95
Last seen: 6 years, 3 months
Re: Good news/bad news time.... [Re: natedawgnow]
    #22315957 - 09/30/15 07:39 PM (8 years, 3 months ago)

Could be, but the contams on the other ones are all near the top of the dry verm layer, and not near the injection sites.  Survey says it was something to do with my inoc procedures...  lids were too loose or somehow shit got in through the dry verm layer.


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Invisiblewowimflabbergasted
supercalifragilistic
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Registered: 07/16/12
Posts: 18,918
Re: Good news/bad news time.... [Re: Ro0k]
    #22316682 - 09/30/15 10:36 PM (8 years, 3 months ago)

How did you sterilize them and why are they in an incubator? What are your temps? Did you use a SAB or just open air?


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