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Offlinetopsykretts
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What else does my still air box need?
    #22313212 - 09/30/15 09:58 AM (8 years, 3 months ago)

Currently it just has two holes in the front for my arms/hands.  I bought some yellow vinyl/rubber kitchen gloves in case I want to attach them and turn it into a glove box.  Do I need to cut another hole for anything?  How do people use an alcohol lamp with this thing?  Seems kinda risky to put an open flame inside a box.  How do I get things into the box? 

What I was wanting to do with it is make some spore-water solution with my spore print which I've been putting off for a very long time.  Just not sure if I'm ready to do that now or not.  I've got syringes, sterilized water in a jar, a spore print, alcohol and a SAB.  Do I need some lysol or oust too?


Edited by topsykretts (09/30/15 09:59 AM)


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InvisibleLocN9ne
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Re: What else does my still air box need? [Re: topsykretts]
    #22313221 - 09/30/15 10:01 AM (8 years, 3 months ago)

The two holes are exactly what you want, no need for anything more...you keep your alcohol lamp or whatever flame sources outside of your box...having a flame inside your box will 1. Be dangerous, 2. Create air currents negating the purpose of a Still Air Box. ..


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InvisibleSkipJames
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Re: What else does my still air box need? [Re: topsykretts]
    #22313266 - 09/30/15 10:15 AM (8 years, 3 months ago)

I used to spray my glovebox with some sort of disinfectant I've mixed by deluting the regular disinfectant for bathrooms with some water. And the gloves too, then I'd rub them together and stick them in.

Thinking back about it now, it seems like it was quite a ghetto-ass SAB, just put together really quickly, but it worked, and there weren't any contam problems with it.


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InvisibleLocN9ne
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Re: What else does my still air box need? [Re: SkipJames]
    #22313283 - 09/30/15 10:20 AM (8 years, 3 months ago)

Yeah oust the room your going to be working in, and spray the inside of your SAB with soapy water...let both the ousted room, and the sprayed SAB sit for about 15 minutes after spraying before you begin.


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Invisiblemushmagic
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Re: What else does my still air box need? [Re: LocN9ne]
    #22313307 - 09/30/15 10:30 AM (8 years, 3 months ago)

I don't even bother with spraying oust or any other sanitizing spray. The SAB is enough so long as you use it properly IMO


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OfflineLogicaL ChaosM
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Re: What else does my still air box need? [Re: topsykretts]
    #22313319 - 09/30/15 10:34 AM (8 years, 3 months ago)

I use a modified tupperware box in the past to make a SAB.

So the top is for loading the box, then you seal the top (it has that foamy tape to seal the edges), then I put something heavy on the top to seal the box.

I would have a lamp nearby, for extra lighting in the box.

I also have an iso alcohol spray bottle inside the box to clean things as needed.


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InvisibleMad Season
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Re: What else does my still air box need? [Re: LogicaL Chaos] * 1
    #22313331 - 09/30/15 10:40 AM (8 years, 3 months ago)

These days all of us recommend a sab. They have a much higher success rate due to the still air. But there's a technique involved. Glove boxes are so 2005.

Read my improve your sterile techniques link in my signature top to bottom. Especially read the part on sabs.


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OfflineLogicaL ChaosM
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Re: What else does my still air box need? [Re: Mad Season]
    #22313338 - 09/30/15 10:43 AM (8 years, 3 months ago)

Are glove boxes obsolete now?

Interesting :strokebeard:


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Offlinetopsykretts
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Re: What else does my still air box need? [Re: LogicaL Chaos]
    #22319445 - 10/01/15 02:33 PM (8 years, 3 months ago)

Would it still be a good idea to use gloves even if they aren't attached to the box?

As far as flame sterilization.. is the iea to sterilize outside of the box, then insert sterilized objects into the box through an arm hole?


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InvisibleMad Season
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Re: What else does my still air box need? [Re: topsykretts]
    #22319489 - 10/01/15 02:43 PM (8 years, 3 months ago)

Yes and yes :lol:


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OfflineiSmkGrnBud
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Re: What else does my still air box need? [Re: topsykretts]
    #22319495 - 10/01/15 02:44 PM (8 years, 3 months ago)

Quote:

topsykretts said:
Would it still be a good idea to use gloves even if they aren't attached to the box?

As far as flame sterilization.. is the iea to sterilize outside of the box, then insert sterilized objects into the box through an arm hole?




I wear gloves. If you wash you're hands, it doesn't really matter. I constantly keep my hands soaked in iso, this is why I prefer gloves.

Yea flame sterilize outside of box, then slowly enter the box through armhole with sterilized utensil. :cheers:


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Offlinetopsykretts
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Re: What else does my still air box need? [Re: iSmkGrnBud]
    #22332528 - 10/04/15 12:41 PM (8 years, 3 months ago)

Someone should really make a video showing exactly how to use a SAB properly.  Its still confusing to me.  I have a hard time visualizing the process and without a clear picture in my mind, I'm apprehensive about attempting it.  On the other hand, the print I got was free.. but I'd hate to contaminate it by doing this improperly.  I guess I'll have to jedi it and hope the force is with me.

Sometimes I think I may be autistic.  I am also disturbed by the sound of people coughing when I walk down the street.  It is as if they are coughing at me.


Edited by topsykretts (10/04/15 12:41 PM)


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OfflineiSmkGrnBud
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Re: What else does my still air box need? [Re: topsykretts]
    #22332536 - 10/04/15 12:42 PM (8 years, 3 months ago)

Quote:

topsykretts said:
Someone should really make a video showing exactly how to use a SAB properly.  Its still confusing to me.  I have a hard time visualizing the process and without a clear picture in my mind, I'm apprehensive about attempting it.  On the other hand, the print I got was free.. but I'd hate to contaminate it by doing this improperly.  I guess I'll have to jedi it and hope the force is with me.

Sometimes I think I may be autistic.  I am also disturbed by the sound of people coughing when I walk down the street.  It is as if they are coughing at me.




Lol :laugh2: http://www.shroomery.org/forums/showflat.php/Number/21889950

Two post up, look in Mad's signature! :cheers:


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InvisibleInocuole
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Re: What else does my still air box need? [Re: iSmkGrnBud]
    #22332610 - 10/04/15 01:00 PM (8 years, 3 months ago)



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