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Offlinekentxxx
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Registered: 09/04/15
Posts: 1
Last seen: 3 years, 5 months
Gallery of contam, green, black, lipstick all that in one batch
    #22312782 - 09/30/15 07:46 AM (8 years, 3 months ago)

This is my first post on the forum, hopefully I am doing things right. Also excuse my English, not my first language.
So I purchased 2 edible mushroom LC syringe, and 1 cubenis spore syring. About 20 some WBS jars were inoculated with them a on 9/21. The jars were PCed for 115 min at 10 psi. In some of the jars I put half wbs and half soil just as an experiment. The jars were cooked about 2 weeks before inoculation, and stays unchanged before inoculation.
After inoculation is a completely different story. It is actually amazing how much contam I can get in a single batch. I thought maybe I could share with you.
Jars from 1st syringe
blue/green mold


I don't know what these are. Gross looking clusters of black dots.

and you can see a lot of metabolite in all these pictures.


Jars from 2nd syringe
Actually zero contam in them, but you do see tons of metabolite secreted by the mycelium.


Cubenis syringe
small patches of green mold only in these jars
This is not long after inoculation (pretty much where I injected)

and now

I think I will just let these sit and see if the mycelium can take over.

Bonus track, lipstick mold or pink mold, not sure which one.
Only in the jars with soil.



At this point I really suspect it is the syringes were contaminated, since all the jars knocked up by an individual syringe have the same type of contamination, and differ from jars inoculated by the other syringes.


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Invisibleazur
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Registered: 04/21/12
Posts: 28,103
Loc: Daid
Re: Gallery of contam, green, black, lipstick all that in one batch [Re: kentxxx]
    #22312898 - 09/30/15 08:18 AM (8 years, 3 months ago)

You gotta pc at 15 psi. And why wait 2 weeks to inoculate? You should do it as soon as they cool.
Also, look into agar


--------------------


A cube is NOT a cube.

FALL IN LOVE WITH LC
FOTTSE!!!
ALL NOOBS READ THIS!!!



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