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Offlineverytastycheese
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AGAR - Contams or mushrooms? Pics!
    #22311174 - 09/29/15 09:53 PM (8 years, 3 months ago)

Alright so I confess I began an agar expriment about 3 months ago and abandoned it for the summer. Spores on agar germinated and grew, so this isn't isolated. Looks like it is growing actual mushroom base material or just a really strong mycelium that wants to pin but hasn't yet (due to no GE?) and I'm wondering if I can use it for transfers or should I toss it?

I'm particularly curious about the brown and clear liquid bubbles on the top - seems like it's sweating out of the growth. Is it 'mycelium piss' / metabolites, or contams?



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InvisiblePeter Pan
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Re: AGAR - Contams or mushrooms? Pics! [Re: verytastycheese]
    #22311290 - 09/29/15 10:26 PM (8 years, 3 months ago)

:eek: don't transfer it


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InvisibleInocuole
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Re: AGAR - Contams or mushrooms? Pics! [Re: Peter Pan]
    #22311301 - 09/29/15 10:30 PM (8 years, 3 months ago)

That metabolites, you can't do anything to save that.


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InvisibleMad Season
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Re: AGAR - Contams or mushrooms? Pics! [Re: Inocuole]
    #22311312 - 09/29/15 10:33 PM (8 years, 3 months ago)

:horrified:

I spy some bacteria and mycogone


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Edited by Mad Season (09/29/15 10:34 PM)


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InvisiblebodhisattaMDiscordReddit
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Re: AGAR - Contams or mushrooms? Pics! [Re: Mad Season]
    #22311350 - 09/29/15 10:43 PM (8 years, 3 months ago)

I spy transfer weeks ago. First sign of growth bro


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Offlineverytastycheese
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Re: AGAR - Contams or mushrooms? Pics! [Re: Mad Season]
    #22311366 - 09/29/15 10:45 PM (8 years, 3 months ago)

Alright I won't touch it, but I have some followup questions. Why does the existence of metabolites mean there's nothing I can do to save it?

And where do you spy bacteria? Any chance you could highlight it for my own education for future identification?

Thanks!


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InvisibleLocN9ne
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Re: AGAR - Contams or mushrooms? Pics! [Re: verytastycheese]
    #22311389 - 09/29/15 10:50 PM (8 years, 3 months ago)



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InvisibleMad Season
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Re: AGAR - Contams or mushrooms? Pics! [Re: LocN9ne]
    #22311482 - 09/29/15 11:11 PM (8 years, 3 months ago)



Black is mycogone and MAYBE some cube myc on the outer edge

Yellow is bacteria, it may look uncolonized, but it's definitely bacteria. Bacteria can sometimes be almost clear and make it look mucusy.

Here's a plate with at least 6 different different types of bacteria, to help you compare:

Here's another one with at least 5 different types of bacteria:

Red is either bacteria, mycogone mycelium, or yeast. It's hard to say without putting it under the scope


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


Edited by Mad Season (09/29/15 11:14 PM)


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Offlineverytastycheese
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Re: AGAR - Contams or mushrooms? Pics! [Re: Mad Season]
    #22482330 - 11/05/15 06:23 PM (8 years, 2 months ago)

Well 2 of the pictured plates ended up forming pins, so I did extract some of the mushroom tissue and placed on new plates, and they seem to be growing healthily! I think part of the problem is this pre-mixed agar though.


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