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OfflineRo0k
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Registered: 08/29/15
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Last seen: 6 years, 3 months
A few questions about agar and coir I haven't been able to find the answers to.
    #22310952 - 09/29/15 09:02 PM (8 years, 3 months ago)

Hi there.  I've been searching for the last day or so for information regarding this, but I haven't been able to find a satisfactory answer (or any, come to think of it) for a few questions I have regarding agar and coir substrate.

Bit of a preamble: I've had contam issues with the last 15 brf cakes I've done, and I can't tell whether it's contamed syringes or piss-poor sterile procedure, so I'm trying to troubleshoot.  Admission: it's probably a bit of both. 

To test, I'm going to try and control as many variables as possible.  If the agar is clean, it's my sterile procedure.  If not, it's the syringes.  To that end I rebuilt my SAB and plan to start testing the remaining syringes on agar.  I'll also be switching to bleach spray for my SAB as I understand bleach has stronger anti-organic properties than rubbing alcohol. 

Also it means I can flame the needle inside the SAB, and no go boom nor will I be worried about possible contams from the torch being outside the box. 

My end goal is to use a mycelium-liquid dealie from the agar to inoc new brf cakes and also give a couple quarts of rye a try for bulk spawn, because what the hell. 

So I've read up on RR's how to do rye grain, how to properly make agar and not have it blow up inside your PC, how to make the agar plates to minimize contams etc.

Here's my remaining questions: 

Once the agar plate colonizes and I squirt in some sterile water, mix it up and suck it back into the syringe, what do I do with the petri dish?  Can I use it still, or do I need to throw it out? 

Last is how much substrate to make.  Let's say I'll be using Damion5050s coir tek, but I won't be using nearly 5 qt of spawn... more like 2, tops.  Is there a ballpark figure somebody can give me wrt how much of a standard coir brick I should use, how much verm I should use and how much water I should use per 1 qt of spawn?  I'm fine with 1/3 of a brick, or in grams.


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OfflineSynKyd
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Re: A few questions about agar and coir I haven't been able to find the answers to. [Re: Ro0k]
    #22310980 - 09/29/15 09:07 PM (8 years, 3 months ago)

Post some pics of your bad jars and the experienced folk can probably tell you......


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Invisiblewowimflabbergasted
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Re: A few questions about agar and coir I haven't been able to find the answers to. [Re: SynKyd]
    #22311011 - 09/29/15 09:15 PM (8 years, 3 months ago)

1 brick of coir hydrates into roughly 8 quarts so just do the math.

Don't use bleach in your SAB and don't flame in your SAB. Just use soapy water or 70% alcohol if you must. :thumbup: Having a flame in your SAB creates air currents which makes your still air box no longer very still.


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OfflineRo0k
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Re: A few questions about agar and coir I haven't been able to find the answers to. [Re: wowimflabbergasted]
    #22312526 - 09/30/15 05:48 AM (8 years, 3 months ago)

Why no bleach? Wouldn't the mist inside trap, kill then sinkbem to the bottom/sides any microbes or mold spores?awouldn't a spritz or two, esp on the jars, syringe etc be a good thing?


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Invisiblewowimflabbergasted
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Re: A few questions about agar and coir I haven't been able to find the answers to. [Re: Ro0k]
    #22312766 - 09/30/15 07:39 AM (8 years, 3 months ago)

It's pointless, alcohol does what we need and it's less toxic than bleach...use 70% though if you do...it best penetrates cell walls to kill the contams. But that's why you want to use soapy water, it best traps contams and either stick them to walls or knocks them to the floor of the SAB.


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OfflineRo0k
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Last seen: 6 years, 3 months
Re: A few questions about agar and coir I haven't been able to find the answers to. [Re: wowimflabbergasted]
    #22314615 - 09/30/15 03:04 PM (8 years, 3 months ago)

So like... Wipe down w/ alcohol then spray soapy water, let sit for 20 mins and go at er?


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Invisiblewowimflabbergasted
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Re: A few questions about agar and coir I haven't been able to find the answers to. [Re: Ro0k]
    #22314818 - 09/30/15 03:43 PM (8 years, 3 months ago)

Yep. You just don't want the water on the walls to dry.


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