Home | Community | Message Board

Cannabis Seeds - Original Sensible Seeds
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: North Spore Bulk Substrate   Mushroom-Hut Mono Tub Substrate   PhytoExtractum Buy Bali Kratom Powder   Kraken Kratom Red Vein Kratom   Original Sensible Seeds Bulk Cannabis Seeds

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Offlinetedoro
ToadStool Tender
Male


Registered: 02/06/15
Posts: 2,206
Last seen: 19 hours, 57 minutes
Help me troubleshoot contamination issues with franks monos
    #22307590 - 09/29/15 11:12 AM (8 years, 4 months ago)

Hello,

This is awkwardly long, but I didn't know how else to write it. my apologies.

I'm a cubensis golden teacher, agar to rye berry myco bags, bucket/hot water pasteurization of coir, franks monotubs type of guy. Three years into this hobby, still have a ton to learn.

I am having troubles with what I believe to be trich (blueish green powdery mold) contaminating my monotubs after the first flush.

I remember reading a dialog between Frank and RR about frank choosing to pull his harvest instead of cutting, because he always got contams if he cut the fruit. RR pointed out that his agar work wasn't perfectly clean. Or at least thats how I remember the dialog going. As I was experiencing the same thing, I spent the last 5 months learning agar in hopes to lessen my problems. (I wasn't using agar prior to that)

Well I'm back at monotubs with what I believe to be clean agar work, and sure enough, I'm getting the same contams after the first flush.

I will list what I think I'm doing wrong, let me know if one of these sound like the culprit. (or simply all of them)

1) Maybe my agar work is harboring trich and I can't see it.

2) I grow in a closet, and I struggle with too high of temps. Currently the closet air is around 80 degrees... and to keep the humidity up, I fear I'm not introducing enough fresh air.

3) My air is 30%ish year round humidity... I try to use a humidifier to get it up around 60%, but even at 60%, I either get cracked caps, or fuzzy bases... there doesn't seem to be a healthy inbetween.

4) I think I am upsetting the monotub with my rehydration technique. I currently pour about 4 cups of water on the surface after first flush, and let it flow down the edges between the plastic bag and the substrate. After an hour or two I carefully turn the tub on edge, holding it delicately with my hand for a minute and let it drain. Then I put it back in fruiting conditions. I think I let it sit too wet, after the draining. The cut stumps seem to dislike the wet and get slimey.

5) I am not very sterile with anything after my grains are fully colonized. No gloves. my tubs are in a clean-ish closet, but on the floor. I've been sometimes using completely open top monotub holes. I sometimes don't clean my fiskar clippers.

Or maybe something else I'm doing to inadvertently create a good mold environment....

Advise?

T


--------------------
--------------------
Deep pour soft agar plates-->bags of WBS-->Low Profile Monos
Clean spawn thread | Put a thermometer on your PC


Extras: Filter Print Post Top
OfflinetaGyo
Strainiac/AMU
Male User Gallery


Registered: 10/16/14
Posts: 18,802
Loc: Journal Land Flag
Last seen: 5 years, 3 months
Re: Help me troubleshoot contamination issues with franks monos [Re: tedoro]
    #22307647 - 09/29/15 11:27 AM (8 years, 4 months ago)

After the first flush sounds like weak spawn.

Cutting or pulling really doesn't make a difference if you have strong myc. Where it makes the most impact is whether you're leaving large holes in the substrate when you're pulling. That's when I'll opt to grab the scissors because those large holes often lead to poor second and third flush pinsets. If you damage the sub and stress it out while harvesting you're more prone to contam but RR was getting at that Frank didn't have a great culture to begin with, hence why I said weak or contamed spawn.

I would take whatever plate you have and transfer it two more times from the edge. Then transfer that once more to a clean plate. Since I don't see the plate I can't tell you where to take these from but if I run into ride-alongs the only thing you can really do is keep trying to transfer from what looks like clean sections.

If you had a pic of your jars or agar work I can confirm or deny.

80 is a little high but it's overall fine. if you can get it to 75 I bet you'd see less trich. Honestly anything above 77 should be avoided IMO, too tricky of an area.

Fuzzy bases means you need to loosen up your top holes. Some fuzzy base is genetic though, I'd need pics. House RH really matters very little if you can get that polyfill dialed in right:
http://www.shroomery.org/forums/showflat.php/Number/17332777
Try a fan in that closet. A small personal one just to move air around.

You're basically doing the Tribunal Dunk Tek. It's fine. Look up tribunal dunk tek and do it like him exactly. IMO, after the first flush if your sub didn't pump out much weight or too many I'd just mist it down. The stumps revert to mycelium after being cut, that's why they're fuzzing up. If they're turning slimy that's not a good sign. I think you have a ride-along or a weak culture.

When you clip shrooms I recommend at least wiping the cutters with alcohol, just to avoid all that nastiness on weakened mycelium points.

I don't use gloves after full colonization either. Shit man, I don't even wash my hands when I work with coir. It's 99.99% probably not your coir and about 99.99% weak spawn.


--------------------
Gyo's Better Grows
TNF Q&A :rockon:
AMU Q&A

Dominus fortunae meae sum


Extras: Filter Print Post Top
Offlinetedoro
ToadStool Tender
Male


Registered: 02/06/15
Posts: 2,206
Last seen: 19 hours, 57 minutes
Re: Help me troubleshoot contamination issues with franks monos [Re: taGyo]
    #22307949 - 09/29/15 12:17 PM (8 years, 4 months ago)

taGyo,

Much thanks for the time to consider my situation. I will focus on improving my spawn. There is a side of me that wishes I could run into a generous chap that has some personally honed cube strain. Because even after I figure this out... I'll need to start getting a good strain going. I have so much to work on. Thanks again for the advise.

T


--------------------
--------------------
Deep pour soft agar plates-->bags of WBS-->Low Profile Monos
Clean spawn thread | Put a thermometer on your PC


Extras: Filter Print Post Top
OfflinetaGyo
Strainiac/AMU
Male User Gallery


Registered: 10/16/14
Posts: 18,802
Loc: Journal Land Flag
Last seen: 5 years, 3 months
Re: Help me troubleshoot contamination issues with franks monos [Re: tedoro]
    #22307983 - 09/29/15 12:25 PM (8 years, 4 months ago)

IMO make that strain :wink:,

It feels so much better after it's your hard work and you have a rockin' clone. Prints are easy to come by and honestly a cube is a cube in growing preferences, find the ones you like to eat and grow 'em till you get an isolate that flushes a lb :rockon:


--------------------
Gyo's Better Grows
TNF Q&A :rockon:
AMU Q&A

Dominus fortunae meae sum


Extras: Filter Print Post Top
Offlinetedoro
ToadStool Tender
Male


Registered: 02/06/15
Posts: 2,206
Last seen: 19 hours, 57 minutes
Re: Help me troubleshoot contamination issues with franks monos [Re: taGyo]
    #22308232 - 09/29/15 01:12 PM (8 years, 4 months ago)

Love it. Wish me luck.


--------------------
--------------------
Deep pour soft agar plates-->bags of WBS-->Low Profile Monos
Clean spawn thread | Put a thermometer on your PC


Extras: Filter Print Post Top
OfflineKizzle
Misanthrope
 User Gallery

Registered: 08/30/11
Posts: 9,855
Last seen: 20 hours, 9 minutes
Trusted Cultivator
Re: Help me troubleshoot contamination issues with franks monos [Re: tedoro]
    #22308314 - 09/29/15 01:27 PM (8 years, 4 months ago)

Quote:

3) My air is 30%ish year round humidity... I try to use a humidifier to get it up around 60%, but even at 60%, I either get cracked caps, or fuzzy bases... there doesn't seem to be a healthy inbetween.



In the tub or in the closet? If you have a closet and you're raising the humidity to 60% the building material of the closet is going to start growing mold.


--------------------


Extras: Filter Print Post Top
InvisibleMad Season
hookers and blackjack
 User Gallery


Registered: 09/16/12
Posts: 12,666
Loc: Canada Flag
Trusted Cultivator
Re: Help me troubleshoot contamination issues with franks monos [Re: tedoro]
    #22308342 - 09/29/15 01:32 PM (8 years, 4 months ago)

http://www.shroomery.org/forums/showflat.php/Number/22020260

Check out this. It's imo the difference between doing agar, and utilizing agar.

Also bacteria in spawn can be from a high initial endospore count. I like to recommend 20 psi for 2 hours on qt jars. It works decently and only has minimal bacteria. Read my signature on what stamets says about bacteria. I'd also read the improve your sterile techniques link too :smile:


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


Extras: Filter Print Post Top
Offlinetedoro
ToadStool Tender
Male


Registered: 02/06/15
Posts: 2,206
Last seen: 19 hours, 57 minutes
Re: Help me troubleshoot contamination issues with franks monos [Re: Kizzle]
    #22308360 - 09/29/15 01:35 PM (8 years, 4 months ago)

Really? Thats very good to know. What is a safe level? 50%.

It seems fine at 60%  concrete floors, plywood and fir 2x4's. Big 1 inch gaps all over to make it leaky. The times I've brought it up to 80%... now that felt like mold potential.

t


--------------------
--------------------
Deep pour soft agar plates-->bags of WBS-->Low Profile Monos
Clean spawn thread | Put a thermometer on your PC


Extras: Filter Print Post Top
Offlinetedoro
ToadStool Tender
Male


Registered: 02/06/15
Posts: 2,206
Last seen: 19 hours, 57 minutes
Re: Help me troubleshoot contamination issues with franks monos [Re: tedoro]
    #22308371 - 09/29/15 01:37 PM (8 years, 4 months ago)

So wait.... If I keep some polyfill in the upper holes, but get my fan to be stronger, does that keep more moisture in the tub than lower fan levels and no polyfill in the upper holes?


--------------------
--------------------
Deep pour soft agar plates-->bags of WBS-->Low Profile Monos
Clean spawn thread | Put a thermometer on your PC


Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: North Spore Bulk Substrate   Mushroom-Hut Mono Tub Substrate   PhytoExtractum Buy Bali Kratom Powder   Kraken Kratom Red Vein Kratom   Original Sensible Seeds Bulk Cannabis Seeds


Similar ThreadsPosterViewsRepliesLast post
* Possible contamination issue Drew 848 4 02/26/04 05:34 PM
by Drew
* Help! Not sure if contaminated... splasher78 919 3 08/12/02 09:02 AM
by kykeon
* Outdoor patches with contaminated cakes? OmegaRed 2,378 14 05/21/03 06:54 PM
by jehst
* 100% Contaminent Free Cultivation tHeMaGe 1,619 6 11/12/01 11:38 AM
by amazinblue
* Re: don't throw out those contaminated jars... JoHnNySc 10,382 9 12/13/99 10:21 PM
by Psilowarrior
* contamination problem abraham 1,667 3 11/07/02 10:51 AM
by abraham
* Contaminated Syringe? Comfortably_Numb 1,300 3 12/07/03 03:27 PM
by Comfortably_Numb
* Re: The Dangers Of Eating From Contaminated Cakes
( 1 2 all )
Sclorch 74,791 23 07/03/00 01:42 PM
by Anonymous

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
422 topic views. 29 members, 246 guests and 57 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.023 seconds spending 0.007 seconds on 14 queries.