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InvisibleNewToCultivation
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Registered: 08/31/15
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Trying to get into agar
    #22306957 - 09/29/15 07:48 AM (8 years, 4 months ago)

I have an agar recipe that I'm ready to use, I'm just not sure how to sterilize everything and get it ready for spores, do I pour my agar into my quarter pint jars and then put the jars in a Pc , or sterilize the jars alone? Also can you inoculate agar with a syringe ?


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OfflineMachiavelliavore
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Re: Trying to get into agar [Re: NewToCultivation]
    #22307005 - 09/29/15 08:04 AM (8 years, 4 months ago)

Well, you can put the agar mixture into the jars and pc aka nopour, or you can put a bottle full of the mix along with the empty jars, then pour them in the sab with the still hot bottle.
I see no reason to use a pour agar ten with jars, as it ads lots more contam prone steps with no benefit I'm aware of.  The point of pour agar is you can whip up ab bottle, then pour shittons of sterile petris.
Just keep swirling the mix as you distribute to your jars before the pc run, as agar powder settles in liquid a bit before it's cooked.

Yes you can use spore solution.  Use one drop, as the more liquid sloshes around, the harder it will be to separate contams.


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I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


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InvisibleNewToCultivation
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Re: Trying to get into agar [Re: Machiavelliavore]
    #22307043 - 09/29/15 08:19 AM (8 years, 4 months ago)

Yeah fuck pouring hot agar that sounds like a son of a bitch! I'm gonna just Pc the jars and agar together and then inoculate in my sab, (which is my very small closet for now) but I don't really want multi strains in my agar I want to isolate a single  genome type of strong rhizomorphic mycelium that I can get consistent fruits from everytime, this why I'm debating to wait until these fruits I have growing get full size and do it from that .


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everything I do or say is for informational purposes only
I do not condone the cultivation or sales of any illegal substances.

Oh yeah, and i fucked bodhisatta's mother.



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Invisibleazur
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Re: Trying to get into agar [Re: NewToCultivation]
    #22307110 - 09/29/15 08:46 AM (8 years, 4 months ago)

Hot pouring ftw


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OfflineMachiavelliavore
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Re: Trying to get into agar [Re: NewToCultivation]
    #22307112 - 09/29/15 08:47 AM (8 years, 4 months ago)

You shouldn't be too concerned with isolating from ms on agar.  The only information you have there is how fast and rhizo a particular sector is growing, not whether it's a good or potent fruiting strain.  People put spores on agar most to get clean healthy growth and grow with ms from there.

Starting from a good fruit or cluster is the prefered way to get something good.


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I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


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Offlinemushpunx
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Re: Trying to get into agar [Re: Machiavelliavore]
    #22307198 - 09/29/15 09:20 AM (8 years, 4 months ago)

Yea I agree, strain isolation is great but its a lot of work, plates and lots of testing.
Id start off using agar to get some clean spawn, then get some clones going and tested.
Work on strain isolation at the same time, but more as a side project.

No pour is fine but when you have a lot of projects going its way more convinent to have stacks of plates on hand ready to use. Pouring isnt very hard, its not as intimidating as it sounds actually I find its harder to get my no pour plates perfect than pouring plates.

Check out pasty plates too.

I use 1/4 pint jars when Im out of plates. I mix up my agar, and then I run the bottle thru the PC for 10-20 mins just to get it melted and mixed up even. Then I pour my jars, and then run them through their PC cycle.
Its just how I like to do it

When you say inoculate the agar with a syringe, are you talking like through an injection port or something? Because if you are going to be working with agar, you are going to have to learn to open the lid haha

I dont just squirt spores onto the agar anymore, it makes a mess of germination. Either squirt the spores onto a sterilized loop or (my preference) onto a sterile cotton swab and then do a little swipe in the middle of the plate.


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OfflineMilofski
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Re: Trying to get into agar [Re: NewToCultivation]
    #22307238 - 09/29/15 09:31 AM (8 years, 4 months ago)

As hot as you can get it without burning your hands will be the best, so that it's all runny and easy to pour


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Offlinekushroom
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Re: Trying to get into agar [Re: Milofski]
    #22307283 - 09/29/15 09:43 AM (8 years, 4 months ago)

:whathesaid:


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InvisibleMad Season
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Re: Trying to get into agar [Re: kushroom]
    #22307304 - 09/29/15 09:49 AM (8 years, 4 months ago)

Quote:

sterilize your agar for 10-15m at 15PSI. once your PC is back at atmospheric pressure. open it up take your jars out. put them in 47ºC water bath. your agar jars are hotter than 47 so the water bath will warm up. once it cools back down to 47ºC then you're ready to pour.




http://www.shroomery.org/forums/showflat.php/Number/21922023/gonew/1#top

don't have to worry about it being too hot if you do this, and you'll see MUCH less condensation in the plate as it cools.


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OfflineCliftonGK1
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Re: Trying to get into agar [Re: Mad Season]
    #22307546 - 09/29/15 10:58 AM (8 years, 4 months ago)

Habit from my laboratory days of pouring plates:

I PC my agar in a 500mL Erlenmeyer flask.  The shape is easier to hold and control the flow rate of the pour as opposed to PCing in pint jars, IMO.
I use a pre-mixed PDA powder from (PM me for vendor name) and prepare it in 200mL batches, which is low enough in the flask to avoid boil over.  It's enough to pour 15 - 20 plates if you're careful.


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