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Offlinecheesie
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SOME growth on agar **updated pics of agar transfers**
    #22306220 - 09/29/15 01:21 AM (8 years, 4 months ago)

Iv had no growth at all from print to agar despite innoculating over 2-3 days ago. This normal?  No signs of anything at all. SAB used. Spores from Mexicana print.


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:peyotespectrum:issues:peyotespectrum:require:peyotespectrum:tissues:peyotespectrum:
:hiltonsex:


Edited by cheesie (10/10/15 04:19 AM)


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OfflinecronicrFacebook
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Re: no growth on agar [Re: cheesie]
    #22306223 - 09/29/15 01:22 AM (8 years, 4 months ago)

2 or 3 days is nothing(you should be able to remember if it was 2 or 3 days ago stoner)just be patient


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Offlinecheesie
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Re: no growth on agar [Re: cronicr]
    #22306241 - 09/29/15 01:29 AM (8 years, 4 months ago)

Quote:

cronicr said:
2 or 3 days is nothing(you should be able to remember if it was 2 or 3 days ago stoner)just be patient




All my days seem to melt into one at the minute. Long days...


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OfflinecronicrFacebook
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Re: no growth on agar [Re: cheesie]
    #22306256 - 09/29/15 01:33 AM (8 years, 4 months ago)

i have these issues....but i love drugs so it's a fair trade


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InvisibleLocN9ne
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Re: no growth on agar [Re: cronicr]
    #22306261 - 09/29/15 01:36 AM (8 years, 4 months ago)

Quote:

cronicr said:
i have these issues....but i love drugs so it's a fair trade



Best shit I've read all week...


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Invisibletahoe
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Re: no growth on agar [Re: LocN9ne]
    #22306326 - 09/29/15 02:10 AM (8 years, 4 months ago)

Start being concerned after the two week mark


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Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


My Legacy
https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987

Teh=The
I need to proofread


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Offlinecheesie
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Re: no growth on agar [Re: tahoe]
    #22306328 - 09/29/15 02:11 AM (8 years, 4 months ago)

The longer it takes the less chance of contams?


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OfflineSksoul
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Re: no growth on agar [Re: cheesie]
    #22307652 - 09/29/15 11:27 AM (8 years, 4 months ago)

Just had spores take off after 25 days... My temps are a bit lower than others', but patience is a virtue


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Invisibletahoe
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Re: no growth on agar [Re: Sksoul]
    #22308408 - 09/29/15 01:43 PM (8 years, 4 months ago)

Time has nothing to do with contams. Usually a contaminate will germinate before the mushroom spores germinate.
There have been many times where I thought it was a contaminate spore germination but it was in fact a mushroom.


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Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


My Legacy
https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987

Teh=The
I need to proofread


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Offlinecheesie
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Re: no growth on agar [Re: tahoe]
    #22322088 - 10/02/15 03:15 AM (8 years, 3 months ago)

How is this looking. Iv done a transfer already so hopefully I will have two plates. The mycelium is very white.



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Offlinemushpunx
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Re: no growth on agar [Re: cheesie]
    #22322170 - 10/02/15 05:10 AM (8 years, 3 months ago)

The first plate does not look good. Anyway you can get a better photo? I might not be seeing it properly


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Offlinecheesie
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Re: no growth on agar [Re: mushpunx]
    #22322177 - 10/02/15 05:14 AM (8 years, 3 months ago)



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Offlinecheesie
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Re: no growth on agar [Re: cheesie]
    #22322181 - 10/02/15 05:15 AM (8 years, 3 months ago)

It's really white unlike contams iv had before .. its where I put the spores so I'm very hopeful.


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Offlinecheesie
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Re: no growth on agar [Re: cheesie]
    #22322264 - 10/02/15 06:10 AM (8 years, 3 months ago)



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Offlinecheesie
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Re: no growth on agar [Re: cheesie]
    #22322278 - 10/02/15 06:14 AM (8 years, 3 months ago)

Should I transfer more of the bits to other slides or is it all contam?


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Offlinemushpunx
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Re: no growth on agar [Re: cheesie]
    #22322869 - 10/02/15 10:03 AM (8 years, 3 months ago)

Well wait Im confused. Is that a plate i nnoculated by spores or is it a plate that you transferd to?

Seeing the better photo it doesn't look how I thought it was. By why is there myc all over the place? Did the wedge you t ransfered get knocked around on the plate?


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Offlinecheesie
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Re: no growth on agar [Re: mushpunx]
    #22323038 - 10/02/15 10:52 AM (8 years, 3 months ago)

That's the spore inoculation.  The first plate you wanted to view. Is it good or bad?


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Offlinemushpunx
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Re: no growth on agar [Re: cheesie]
    #22323088 - 10/02/15 11:06 AM (8 years, 3 months ago)

Oh you're good Mann

My misunderstanding I thought that was a first transfer plate not germination


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Edited by mushpunx (10/02/15 11:13 AM)


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Offlinecheesie
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Re: no growth on agar [Re: mushpunx]
    #22323226 - 10/02/15 11:38 AM (8 years, 3 months ago)

Does it look promising? Worried it might be contam


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InvisibleMad Season
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Re: no growth on agar [Re: cheesie]
    #22323285 - 10/02/15 11:55 AM (8 years, 3 months ago)

Looks good to me. Especially the big one on the left


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Offlinecheesie
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Re: no growth on agar [Re: Mad Season]
    #22323666 - 10/02/15 01:05 PM (8 years, 3 months ago)

Quote:

Mad Season said:
Looks good to me. Especially the big one on the left





Should I transfer it out to another plate?


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Offlinetripdawg420
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Re: no growth on agar [Re: cheesie]
    #22323678 - 10/02/15 01:07 PM (8 years, 3 months ago)

ya thats bacteria try to save it :thumbup:


--------------------
HUSTLER
How U Survive This Life Everyday Resourcefully
epic GT mono tub
http://www.shroomery.org/forums/showflat.php/Number/17277772

wbs tek
http://www.shroomery.org/forums/showflat.php/Number/11525679
coir tek
http://www.shroomery.org/forums/showflat.php/Number/11917410
results :thumbup:


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Offlinecheesie
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Re: no growth on agar [Re: tripdawg420]
    #22323754 - 10/02/15 01:23 PM (8 years, 3 months ago)

Quote:

tripdawg420 said:
ya thats bacteria try to save it :thumbup:





Is it ?


--------------------
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Offlinetripdawg420
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Re: no growth on agar [Re: cheesie]
    #22323786 - 10/02/15 01:31 PM (8 years, 3 months ago)

ya that milky white shit


--------------------
HUSTLER
How U Survive This Life Everyday Resourcefully
epic GT mono tub
http://www.shroomery.org/forums/showflat.php/Number/17277772

wbs tek
http://www.shroomery.org/forums/showflat.php/Number/11525679
coir tek
http://www.shroomery.org/forums/showflat.php/Number/11917410
results :thumbup:


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Offlinekosmokratorshaman
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Re: no growth on agar [Re: tripdawg420]
    #22323976 - 10/02/15 02:19 PM (8 years, 3 months ago)

OP, I am a newb in this world...but that larger thicker looking spot and the one next it look like prime candidates for transfer. Everything to the right and top in the photo in the liquid looks contaminated to me.


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Invisibletahoe
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Re: no growth on agar [Re: kosmokratorshaman]
    #22324365 - 10/02/15 03:35 PM (8 years, 3 months ago)

I would wait another day or two and then transfer from one of the two big blobs in the center.
Stop peaking into the petri to take pictures


--------------------
Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


My Legacy
https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987

Teh=The
I need to proofread


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Offlinecheesie
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Re: no growth on agar [Re: tahoe]
    #22324523 - 10/02/15 04:24 PM (8 years, 3 months ago)

Quote:

tahoe said:
I would wait another day or two and then transfer from one of the two big blobs in the center.
Stop peaking into the petri to take pictures





Will do .


--------------------
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Offlinecheesie
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Re: no growth on agar [Re: cheesie]
    #22331446 - 10/04/15 06:14 AM (8 years, 3 months ago)

I have just done 6 transfers from the original plate. Waiting time now for them to get going.

What do I do then?


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OfflinePsilosoulful

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Re: no growth on agar [Re: cheesie]
    #22331469 - 10/04/15 06:30 AM (8 years, 3 months ago)

Quote:

cheesie said:
I have just done 6 transfers from the original plate. Waiting time now for them to get going.

What do I do then?



Isolate further to find a monoculture if you want (might take awhile) or put a wedge on grains and grow it out. After that, restart the process by cloning the best performing mushroom, and isolate again! :super:


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OfflinetaGyo
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Re: no growth on agar [Re: cheesie]
    #22331470 - 10/04/15 06:31 AM (8 years, 3 months ago)

If this is your second or third transfer I'd start thinking about what grains I wanted to use :rockon:


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Offlinecheesie
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Re: no growth on agar [Re: taGyo]
    #22331506 - 10/04/15 06:49 AM (8 years, 3 months ago)

Quote:

taGyo said:
If this is your second or third transfer I'd start thinking about what grains I wanted to use :rockon:




It's my first transfer but iv got both popcorn and wbs for when I'm ready. Which is best?


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OfflinetaGyo
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Re: no growth on agar [Re: cheesie]
    #22331514 - 10/04/15 06:52 AM (8 years, 3 months ago)

I really hate popcorn, I think it's huge grains that take up a lot of space, look like shit colonized and tend to pop while hydrating or in the PC.

Plenty of great WBS teks:
http://www.shroomery.org/forums/showflat.php/Number/17252080/fpart/all/vc/1

The point of spawn is that each individual kernel becomes an inoculation point in a bulk substrate. In theory (And practice :tongue2:) the smaller the kernel size the quicker it would colonize a tub. If I had a jar full of marbles and a jar full of a brick the brick would have to expand and slowly colonize everything while the marbles would just expand and meet each other.

With that long-winded response out of the way, go WBS :super:


--------------------
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TNF Q&A :rockon:
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Offlinecheesie
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Re: no growth on agar [Re: Psilosoulful]
    #22331517 - 10/04/15 06:53 AM (8 years, 3 months ago)





Isolate further to find a monoculture if you want (might take awhile):super:




What am I looking for in a good monoculture?


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OfflinetaGyo
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Re: no growth on agar [Re: cheesie]
    #22331534 - 10/04/15 07:02 AM (8 years, 3 months ago)

Good fruiting potential, fast colonizer, great pinsets, 2-3 flushes. Monos are great, it's just tough to isolate and isolate without knowing fruiting potential IMO.


--------------------
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TNF Q&A :rockon:
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Dominus fortunae meae sum


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OfflinePsilosoulful

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Re: no growth on agar [Re: taGyo]
    #22331597 - 10/04/15 07:38 AM (8 years, 3 months ago)

:whathesaid: Also, you can try oats, it's cheap, simple, and there's a bunch of teks out there.


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OfflinetaGyo
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Re: no growth on agar [Re: Psilosoulful]
    #22331633 - 10/04/15 08:00 AM (8 years, 3 months ago)

I love me some oats :hehehe:


--------------------
Gyo's Better Grows
TNF Q&A :rockon:
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Dominus fortunae meae sum


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OfflinePsilosoulful

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Re: no growth on agar [Re: taGyo]
    #22331644 - 10/04/15 08:06 AM (8 years, 3 months ago)

Hell yeah!! :bender:


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Offlinecheesie
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Re: no growth on agar [Re: Psilosoulful]
    #22331913 - 10/04/15 09:35 AM (8 years, 3 months ago)

Once these transfers take I will post some more pics and you guys can help me choose the next transfer... if you will help


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OfflinetaGyo
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Re: no growth on agar [Re: cheesie]
    #22331978 - 10/04/15 10:02 AM (8 years, 3 months ago)

:kenthumbup:


--------------------
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TNF Q&A :rockon:
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Dominus fortunae meae sum


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Offlinecheesie
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Re: no growth on agar [Re: taGyo]
    #22357606 - 10/10/15 04:00 AM (8 years, 3 months ago)

Some of the transfers... what do you all think?




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InvisibleSupalemonhaze
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Re: no growth on agar [Re: cheesie]
    #22357620 - 10/10/15 04:08 AM (8 years, 3 months ago)

Why does his agar look like tar? And why does his mycelium look like boils on agar? xD. Nothing like I ever saw


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Offlinecheesie
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Re: no growth on agar [Re: Supalemonhaze]
    #22357630 - 10/10/15 04:12 AM (8 years, 3 months ago)

Quote:

Supalemonhaze said:
Why does his agar look like tar? And why does his mycelium look like boils on agar? xD. Nothing like I ever saw




The agar was coloured black with food colouring and the lumps in the middle are agar transfer wedges


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Offlinecheesie
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Re: no growth on agar [Re: cheesie]
    #22357633 - 10/10/15 04:15 AM (8 years, 3 months ago)

The colouring of the agar helps to see whats going on the plate


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InvisibleLocN9ne
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Re: no growth on agar [Re: cheesie]
    #22357646 - 10/10/15 04:29 AM (8 years, 3 months ago)

I am no pro... but plates 4 and 5 look promising to me... they took off pretty quick... give it a day or three and see what your working with.


--------------------


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Offlinecheesie
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Re: no growth on agar [Re: LocN9ne]
    #22357694 - 10/10/15 05:30 AM (8 years, 3 months ago)

Oh some lc as well... the dark clumps are spores as iv watched the grow each day



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InvisibleSupalemonhaze
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Re: no growth on agar [Re: cheesie]
    #22357707 - 10/10/15 05:42 AM (8 years, 3 months ago)

Quote:

cheesie said:
Quote:

Supalemonhaze said:
Why does his agar look like tar? And why does his mycelium look like boils on agar? xD. Nothing like I ever saw






The agar was coloured black with food colouring and the lumps in the middle are agar transfer wedges






Ive seen coloured agar before but dang nothing like that xD. Mycelium seems strange to me cuz its growing vertical a lot. Mine stays pretty flat


Edited by Supalemonhaze (10/10/15 05:45 AM)


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Offlinecheesie
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Re: no growth on agar [Re: Supalemonhaze]
    #22357708 - 10/10/15 05:46 AM (8 years, 3 months ago)

I think the transfered slants might have been a little big. Reading up people recommend only a grain of rice size transfer.. but we learn. The mycelium has literally covered the transfer agar that's why it looks like a bump and seems to be travelling skyward


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InvisibleSupalemonhaze
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Re: no growth on agar [Re: cheesie]
    #22357713 - 10/10/15 05:53 AM (8 years, 3 months ago)

Quote:

cheesie said:
I think the transfered slants might have been a little big. Reading up people recommend only a grain of rice size transfer.. but we learn. The mycelium has literally covered the transfer agar that's why it looks like a bump and seems to be travelling skyward





Ahh I see. Good luck getting that ferocious isolate!!


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Offlinecheesie
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Re: no growth on agar [Re: Supalemonhaze]
    #22357718 - 10/10/15 05:58 AM (8 years, 3 months ago)

Yeah think I may be isolating again and again. But I'm enjoying agar work its pretty cool. Can't stop looking at the plates.


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InvisibleSupalemonhaze
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Re: no growth on agar [Re: cheesie]
    #22357727 - 10/10/15 06:04 AM (8 years, 3 months ago)

Quote:

cheesie said:
Yeah think I may be isolating again and again. But I'm enjoying agar work its pretty cool. Can't stop looking at the plates.




Aye me too. I am loving this new hobby. Has got the best fruits of my labour thing going on.

I know right?? I try not to look too much cuz I think the dark is better for the growing mycelium but 2 or 3 times a day I cant help it.  That is where guns n roses "patience" tries to help be but I guess I better try to find some online lol.


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OfflinePsilosoulful

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Re: no growth on agar [Re: Supalemonhaze]
    #22357748 - 10/10/15 06:17 AM (8 years, 3 months ago)

Cheesie, Looks like you poured that agar a bit too thick. It'll still grow out fine, but you're gonna have to really dig into it to get a wedge out for your transfers.

Pour enough to line the bottom, I'd say about half of what you have there, then PC.


Edited by Psilosoulful (10/10/15 06:19 AM)


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Offlinecheesie
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Re: no growth on agar [Re: Psilosoulful]
    #22357762 - 10/10/15 06:23 AM (8 years, 3 months ago)

Quote:

Psilosoulful said:
Cheesie, Looks like you poured that agar a bit too thick. It'll still grow out fine, but you're gonna have to really dig into it to get a wedge out for your transfers.

Pour enough to line the bottom, I'd say about half of what you have there, then PC.





Thanks will do. I noticed I was transferring boulders because of how much agar I had put in


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OfflinePsilosoulful

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Re: no growth on agar [Re: cheesie]
    #22357781 - 10/10/15 06:36 AM (8 years, 3 months ago)

Quote:

cheesie said:
Quote:

Psilosoulful said:
Cheesie, Looks like you poured that agar a bit too thick. It'll still grow out fine, but you're gonna have to really dig into it to get a wedge out for your transfers.

Pour enough to line the bottom, I'd say about half of what you have there, then PC.





Thanks will do. I noticed I was transferring boulders because of how much agar I had put in



:lol:


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Offlinecheesie
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Re: no growth on agar [Re: Psilosoulful]
    #22372668 - 10/13/15 10:48 AM (8 years, 3 months ago)

When do I transfer again? My plates don't seem to be spreading out as much and iv lost two plates to contam. Do i wait till I see the threads etc


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OfflinePsilosoulful

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Re: no growth on agar [Re: cheesie]
    #22373645 - 10/13/15 02:19 PM (8 years, 3 months ago)

Transfer clean growth from the leading edge of the mycelium. You won't always see threading as it's dependent on genetics (needs to be isolated further) and the nutritional profile of the agar itself.


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