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cheesie
supreme friendly being



Registered: 07/03/06
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SOME growth on agar **updated pics of agar transfers**
#22306220 - 09/29/15 01:21 AM (8 years, 4 months ago) |
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Iv had no growth at all from print to agar despite innoculating over 2-3 days ago. This normal? No signs of anything at all. SAB used. Spores from Mexicana print.
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Edited by cheesie (10/10/15 04:19 AM)
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cronicr



Registered: 08/07/11
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Re: no growth on agar [Re: cheesie]
#22306223 - 09/29/15 01:22 AM (8 years, 4 months ago) |
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2 or 3 days is nothing(you should be able to remember if it was 2 or 3 days ago stoner)just be patient
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cheesie
supreme friendly being



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Re: no growth on agar [Re: cronicr]
#22306241 - 09/29/15 01:29 AM (8 years, 4 months ago) |
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Quote:
cronicr said: 2 or 3 days is nothing(you should be able to remember if it was 2 or 3 days ago stoner)just be patient
All my days seem to melt into one at the minute. Long days...
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cronicr



Registered: 08/07/11
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Re: no growth on agar [Re: cheesie]
#22306256 - 09/29/15 01:33 AM (8 years, 4 months ago) |
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i have these issues....but i love drugs so it's a fair trade
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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LocN9ne
ɢᄋᄋd ԲᄋЯ ᄁᄋȚᅢΙᄁɢ ᄂᄋ₩ᄂΙԲᄐ



Registered: 04/17/15
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Loc: to the brain
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Re: no growth on agar [Re: cronicr]
#22306261 - 09/29/15 01:36 AM (8 years, 4 months ago) |
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Quote:
cronicr said: i have these issues....but i love drugs so it's a fair trade
Best shit I've read all week...
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Q&A US vs. THEM The more I learn, the less I know.
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tahoe
Noob Slayer



Registered: 11/26/03
Posts: 6,274
Loc: N38.93829W119.98108
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Re: no growth on agar [Re: LocN9ne]
#22306326 - 09/29/15 02:10 AM (8 years, 4 months ago) |
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Start being concerned after the two week mark
-------------------- Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.
My Legacy https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987 Teh=The I need to proofread
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cheesie
supreme friendly being



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Re: no growth on agar [Re: tahoe]
#22306328 - 09/29/15 02:11 AM (8 years, 4 months ago) |
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The longer it takes the less chance of contams?
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Sksoul
Pan handler



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Re: no growth on agar [Re: cheesie]
#22307652 - 09/29/15 11:27 AM (8 years, 4 months ago) |
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Just had spores take off after 25 days... My temps are a bit lower than others', but patience is a virtue
-------------------- Like all great travellers, I have seen more than I remember, and remember more than I have seen.
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tahoe
Noob Slayer



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Re: no growth on agar [Re: Sksoul]
#22308408 - 09/29/15 01:43 PM (8 years, 4 months ago) |
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Time has nothing to do with contams. Usually a contaminate will germinate before the mushroom spores germinate. There have been many times where I thought it was a contaminate spore germination but it was in fact a mushroom.
-------------------- Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.
My Legacy https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987 Teh=The I need to proofread
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cheesie
supreme friendly being



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Re: no growth on agar [Re: tahoe]
#22322088 - 10/02/15 03:15 AM (8 years, 3 months ago) |
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How is this looking. Iv done a transfer already so hopefully I will have two plates. The mycelium is very white.
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mushpunx
Fungus Punk



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Re: no growth on agar [Re: cheesie]
#22322170 - 10/02/15 05:10 AM (8 years, 3 months ago) |
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The first plate does not look good. Anyway you can get a better photo? I might not be seeing it properly
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cheesie
supreme friendly being



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Re: no growth on agar [Re: mushpunx]
#22322177 - 10/02/15 05:14 AM (8 years, 3 months ago) |
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cheesie
supreme friendly being



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Re: no growth on agar [Re: cheesie]
#22322181 - 10/02/15 05:15 AM (8 years, 3 months ago) |
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It's really white unlike contams iv had before .. its where I put the spores so I'm very hopeful.
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cheesie
supreme friendly being



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Re: no growth on agar [Re: cheesie]
#22322264 - 10/02/15 06:10 AM (8 years, 3 months ago) |
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cheesie
supreme friendly being



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Re: no growth on agar [Re: cheesie]
#22322278 - 10/02/15 06:14 AM (8 years, 3 months ago) |
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Should I transfer more of the bits to other slides or is it all contam?
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mushpunx
Fungus Punk



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Re: no growth on agar [Re: cheesie]
#22322869 - 10/02/15 10:03 AM (8 years, 3 months ago) |
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Well wait Im confused. Is that a plate i nnoculated by spores or is it a plate that you transferd to?
Seeing the better photo it doesn't look how I thought it was. By why is there myc all over the place? Did the wedge you t ransfered get knocked around on the plate?
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cheesie
supreme friendly being



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Re: no growth on agar [Re: mushpunx]
#22323038 - 10/02/15 10:52 AM (8 years, 3 months ago) |
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That's the spore inoculation. The first plate you wanted to view. Is it good or bad?
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mushpunx
Fungus Punk



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Re: no growth on agar [Re: cheesie]
#22323088 - 10/02/15 11:06 AM (8 years, 3 months ago) |
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Oh you're good Mann
My misunderstanding I thought that was a first transfer plate not germination
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 Amateur Mycologists United AMU Q&A
Edited by mushpunx (10/02/15 11:13 AM)
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cheesie
supreme friendly being



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Re: no growth on agar [Re: mushpunx]
#22323226 - 10/02/15 11:38 AM (8 years, 3 months ago) |
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Does it look promising? Worried it might be contam
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Mad Season
hookers and blackjack



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Re: no growth on agar [Re: cheesie]
#22323285 - 10/02/15 11:55 AM (8 years, 3 months ago) |
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Looks good to me. Especially the big one on the left
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cheesie
supreme friendly being



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Quote:
Mad Season said: Looks good to me. Especially the big one on the left
Should I transfer it out to another plate?
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tripdawg420
low life with no life



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Re: no growth on agar [Re: cheesie]
#22323678 - 10/02/15 01:07 PM (8 years, 3 months ago) |
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ya thats bacteria try to save it
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cheesie
supreme friendly being



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Quote:
tripdawg420 said: ya thats bacteria try to save it 
Is it ?
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tripdawg420
low life with no life



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Re: no growth on agar [Re: cheesie]
#22323786 - 10/02/15 01:31 PM (8 years, 3 months ago) |
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ya that milky white shit
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kosmokratorshaman
Cosmic Creator


Registered: 05/13/15
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OP, I am a newb in this world...but that larger thicker looking spot and the one next it look like prime candidates for transfer. Everything to the right and top in the photo in the liquid looks contaminated to me.
-------------------- I am.
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tahoe
Noob Slayer



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I would wait another day or two and then transfer from one of the two big blobs in the center. Stop peaking into the petri to take pictures
-------------------- Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.
My Legacy https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987 Teh=The I need to proofread
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cheesie
supreme friendly being



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Re: no growth on agar [Re: tahoe]
#22324523 - 10/02/15 04:24 PM (8 years, 3 months ago) |
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Quote:
tahoe said: I would wait another day or two and then transfer from one of the two big blobs in the center. Stop peaking into the petri to take pictures
Will do .
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cheesie
supreme friendly being



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Re: no growth on agar [Re: cheesie]
#22331446 - 10/04/15 06:14 AM (8 years, 3 months ago) |
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I have just done 6 transfers from the original plate. Waiting time now for them to get going.
What do I do then?
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Psilosoulful

Registered: 09/05/14
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Re: no growth on agar [Re: cheesie]
#22331469 - 10/04/15 06:30 AM (8 years, 3 months ago) |
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Quote:
cheesie said: I have just done 6 transfers from the original plate. Waiting time now for them to get going.
What do I do then?
Isolate further to find a monoculture if you want (might take awhile) or put a wedge on grains and grow it out. After that, restart the process by cloning the best performing mushroom, and isolate again!
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taGyo
Strainiac/AMU



Registered: 10/16/14
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Re: no growth on agar [Re: cheesie]
#22331470 - 10/04/15 06:31 AM (8 years, 3 months ago) |
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If this is your second or third transfer I'd start thinking about what grains I wanted to use
-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
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cheesie
supreme friendly being



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Re: no growth on agar [Re: taGyo]
#22331506 - 10/04/15 06:49 AM (8 years, 3 months ago) |
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Quote:
taGyo said: If this is your second or third transfer I'd start thinking about what grains I wanted to use 
It's my first transfer but iv got both popcorn and wbs for when I'm ready. Which is best?
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taGyo
Strainiac/AMU



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Re: no growth on agar [Re: cheesie]
#22331514 - 10/04/15 06:52 AM (8 years, 3 months ago) |
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I really hate popcorn, I think it's huge grains that take up a lot of space, look like shit colonized and tend to pop while hydrating or in the PC.
Plenty of great WBS teks: http://www.shroomery.org/forums/showflat.php/Number/17252080/fpart/all/vc/1
The point of spawn is that each individual kernel becomes an inoculation point in a bulk substrate. In theory (And practice ) the smaller the kernel size the quicker it would colonize a tub. If I had a jar full of marbles and a jar full of a brick the brick would have to expand and slowly colonize everything while the marbles would just expand and meet each other.
With that long-winded response out of the way, go WBS
-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
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cheesie
supreme friendly being



Registered: 07/03/06
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Isolate further to find a monoculture if you want (might take awhile)
What am I looking for in a good monoculture?
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taGyo
Strainiac/AMU



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Re: no growth on agar [Re: cheesie]
#22331534 - 10/04/15 07:02 AM (8 years, 3 months ago) |
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Good fruiting potential, fast colonizer, great pinsets, 2-3 flushes. Monos are great, it's just tough to isolate and isolate without knowing fruiting potential IMO.
-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
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Psilosoulful

Registered: 09/05/14
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Re: no growth on agar [Re: taGyo]
#22331597 - 10/04/15 07:38 AM (8 years, 3 months ago) |
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Also, you can try oats, it's cheap, simple, and there's a bunch of teks out there.
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taGyo
Strainiac/AMU



Registered: 10/16/14
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I love me some oats
-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
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Psilosoulful

Registered: 09/05/14
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Re: no growth on agar [Re: taGyo]
#22331644 - 10/04/15 08:06 AM (8 years, 3 months ago) |
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Hell yeah!!
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cheesie
supreme friendly being



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Once these transfers take I will post some more pics and you guys can help me choose the next transfer... if you will help
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taGyo
Strainiac/AMU



Registered: 10/16/14
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Re: no growth on agar [Re: cheesie]
#22331978 - 10/04/15 10:02 AM (8 years, 3 months ago) |
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-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
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cheesie
supreme friendly being



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Re: no growth on agar [Re: taGyo]
#22357606 - 10/10/15 04:00 AM (8 years, 3 months ago) |
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Some of the transfers... what do you all think?
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
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Re: no growth on agar [Re: cheesie]
#22357620 - 10/10/15 04:08 AM (8 years, 3 months ago) |
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Why does his agar look like tar? And why does his mycelium look like boils on agar? xD. Nothing like I ever saw
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cheesie
supreme friendly being



Registered: 07/03/06
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Quote:
Supalemonhaze said: Why does his agar look like tar? And why does his mycelium look like boils on agar? xD. Nothing like I ever saw
The agar was coloured black with food colouring and the lumps in the middle are agar transfer wedges
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cheesie
supreme friendly being



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Re: no growth on agar [Re: cheesie]
#22357633 - 10/10/15 04:15 AM (8 years, 3 months ago) |
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The colouring of the agar helps to see whats going on the plate
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LocN9ne
ɢᄋᄋd ԲᄋЯ ᄁᄋȚᅢΙᄁɢ ᄂᄋ₩ᄂΙԲᄐ



Registered: 04/17/15
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Re: no growth on agar [Re: cheesie]
#22357646 - 10/10/15 04:29 AM (8 years, 3 months ago) |
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I am no pro... but plates 4 and 5 look promising to me... they took off pretty quick... give it a day or three and see what your working with.
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cheesie
supreme friendly being



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Re: no growth on agar [Re: LocN9ne]
#22357694 - 10/10/15 05:30 AM (8 years, 3 months ago) |
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Oh some lc as well... the dark clumps are spores as iv watched the grow each day
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
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Loc: 12" down Europe's butthole
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Re: no growth on agar [Re: cheesie]
#22357707 - 10/10/15 05:42 AM (8 years, 3 months ago) |
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Quote:
cheesie said:
Quote:
Supalemonhaze said: Why does his agar look like tar? And why does his mycelium look like boils on agar? xD. Nothing like I ever saw
The agar was coloured black with food colouring and the lumps in the middle are agar transfer wedges
Ive seen coloured agar before but dang nothing like that xD. Mycelium seems strange to me cuz its growing vertical a lot. Mine stays pretty flat
Edited by Supalemonhaze (10/10/15 05:45 AM)
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cheesie
supreme friendly being



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I think the transfered slants might have been a little big. Reading up people recommend only a grain of rice size transfer.. but we learn. The mycelium has literally covered the transfer agar that's why it looks like a bump and seems to be travelling skyward
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
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Re: no growth on agar [Re: cheesie]
#22357713 - 10/10/15 05:53 AM (8 years, 3 months ago) |
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Quote:
cheesie said: I think the transfered slants might have been a little big. Reading up people recommend only a grain of rice size transfer.. but we learn. The mycelium has literally covered the transfer agar that's why it looks like a bump and seems to be travelling skyward
Ahh I see. Good luck getting that ferocious isolate!!
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cheesie
supreme friendly being



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Yeah think I may be isolating again and again. But I'm enjoying agar work its pretty cool. Can't stop looking at the plates.
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
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Loc: 12" down Europe's butthole
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Re: no growth on agar [Re: cheesie]
#22357727 - 10/10/15 06:04 AM (8 years, 3 months ago) |
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Quote:
cheesie said: Yeah think I may be isolating again and again. But I'm enjoying agar work its pretty cool. Can't stop looking at the plates.
Aye me too. I am loving this new hobby. Has got the best fruits of my labour thing going on.
I know right?? I try not to look too much cuz I think the dark is better for the growing mycelium but 2 or 3 times a day I cant help it. That is where guns n roses "patience" tries to help be but I guess I better try to find some online lol.
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Psilosoulful

Registered: 09/05/14
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Cheesie, Looks like you poured that agar a bit too thick. It'll still grow out fine, but you're gonna have to really dig into it to get a wedge out for your transfers.
Pour enough to line the bottom, I'd say about half of what you have there, then PC.
Edited by Psilosoulful (10/10/15 06:19 AM)
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cheesie
supreme friendly being



Registered: 07/03/06
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Quote:
Psilosoulful said: Cheesie, Looks like you poured that agar a bit too thick. It'll still grow out fine, but you're gonna have to really dig into it to get a wedge out for your transfers.
Pour enough to line the bottom, I'd say about half of what you have there, then PC.
Thanks will do. I noticed I was transferring boulders because of how much agar I had put in
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Psilosoulful

Registered: 09/05/14
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Re: no growth on agar [Re: cheesie]
#22357781 - 10/10/15 06:36 AM (8 years, 3 months ago) |
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Quote:
cheesie said:
Quote:
Psilosoulful said: Cheesie, Looks like you poured that agar a bit too thick. It'll still grow out fine, but you're gonna have to really dig into it to get a wedge out for your transfers.
Pour enough to line the bottom, I'd say about half of what you have there, then PC.
Thanks will do. I noticed I was transferring boulders because of how much agar I had put in
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cheesie
supreme friendly being



Registered: 07/03/06
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When do I transfer again? My plates don't seem to be spreading out as much and iv lost two plates to contam. Do i wait till I see the threads etc
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Psilosoulful

Registered: 09/05/14
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Re: no growth on agar [Re: cheesie]
#22373645 - 10/13/15 02:19 PM (8 years, 3 months ago) |
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Transfer clean growth from the leading edge of the mycelium. You won't always see threading as it's dependent on genetics (needs to be isolated further) and the nutritional profile of the agar itself.
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