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OkieFunguy
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Registered: 09/24/15
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Contamination question
#22303977 - 09/28/15 04:54 PM (8 years, 4 months ago) |
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I have one jar that has cloudy white covering most of the top half and one spot has some dark green mold on it. Obviously I can tell that this jar is toast, but I'm just curious as to what type of contam it is and if it can harm my other jars or not (I have the dry verm layer on top of each jar). Also I am posting a pic of another jar because with this being my first time, I am not sure if it is mycelium or not. Any help with these concerns would be appreciated.

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iSmkGrnBud
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Re: Contamination question [Re: OkieFunguy]
#22304016 - 09/28/15 05:02 PM (8 years, 4 months ago) |
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The top one looks like sporulated aspergillus niger. Wait for someone else with a definite answer. I would definitely remove it from where your other jars are. Personally I would PC then dump, or simply just toss the whole thing. It's no good anymore.
Yes that second picture has mycelium in it
-------------------- iSmkGrnBud's Teks iSmkGrnBud's Wild Mushroom Finds  The Noob Forum's List of Teks “The probability of success is difficult to estimate; but if we never search the chance of success is zero.” -Giuseppe Cocconi
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OkieFunguy
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Re: Contamination question [Re: iSmkGrnBud]
#22304182 - 09/28/15 05:40 PM (8 years, 4 months ago) |
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I've already removed the contaminated jar and put it in my garage for now. I have 4 jars with the small patches of mycelium and the remaining 5 jars have nothing yet. It's been 8 days since inoculation. I did this 10 jar batch by sterilizing in a pot on the stove, and then I went and got a PC and inoculated 12 more jars yesterday. I don't know if you can tell from the pic, but the one with mycelium looks like it has some brown specs on top of the white, is that possible mold as well?
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WolfieNuke
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Re: Contamination question [Re: OkieFunguy]
#22304288 - 09/28/15 06:06 PM (8 years, 4 months ago) |
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Quote:
OkieFunguy said: I don't know if you can tell from the pic, but the one with mycelium looks like it has some brown specs on top of the white, is that possible mold as well?
Sometimes vermiculite that is against the glass will not be covered by mycelium, and will look like brown specks. Take a look at it again with that in mind, and see if that is what it is... I'm sure it's just verm.
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OkieFunguy
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Re: Contamination question [Re: WolfieNuke]
#22304407 - 09/28/15 06:31 PM (8 years, 4 months ago) |
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That looks like it could be verm for sure. Just being cautious since one jar is obviously contaminated
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iSmkGrnBud
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Re: Contamination question [Re: OkieFunguy]
#22304457 - 09/28/15 06:40 PM (8 years, 4 months ago) |
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Just keep a good eye on them for the next couple of days. Refrain from touching them a lot.
-------------------- iSmkGrnBud's Teks iSmkGrnBud's Wild Mushroom Finds  The Noob Forum's List of Teks “The probability of success is difficult to estimate; but if we never search the chance of success is zero.” -Giuseppe Cocconi
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OkieFunguy
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Re: Contamination question [Re: OkieFunguy]
#22351434 - 10/08/15 06:45 PM (8 years, 3 months ago) |
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Okay so I have an update to this post. I threw out that first contaminated jar. Here's a back story on my grow process: I did 10 jars sterilized in a pot, and then went and bought a pressure cooker and did 12 more jars. The jars sterilized in the pot are doing way better than the pressure cooker jars. I must have done something wrong with the pressure cooker. But anyway, I have 5 jars with healthy mycelium growth from the pot sterilized batch and one additional jar from that batch has growth but does not look like the other. The 5 jars I know are good because they all have strong rhizomorphic growth. But this other jar has cloudy, off white or grayish growth. I am thinking this jar may be cobweb but with this being my first time I am not sure. Here are the pics of one healthy rhizo jar and then the cloudy jar. The jar on the left is the one with the cloudy growth, the one on the right is the one I know is healthy. If anyone can explain the difference I would appreciate it.
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Coraltrout
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Re: Contamination question [Re: OkieFunguy]
#22351470 - 10/08/15 06:50 PM (8 years, 3 months ago) |
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You've got some awesome rizo going on the jar on the right, make sure you clone a cluster from that jar.
-------------------- 'For it's the end of history, it's caged and frozen still, there is no other pill to take, so swallow the one that makes you ill'
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OkieFunguy
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Re: Contamination question [Re: Coraltrout]
#22351492 - 10/08/15 06:56 PM (8 years, 3 months ago) |
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I've got 5 jars that look exactly like that one
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OkieFunguy
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Re: Contamination question [Re: OkieFunguy]
#22351538 - 10/08/15 07:04 PM (8 years, 3 months ago) |
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I'm just curious about the jar on the left. I didn't know if maybe that was a different type of mycelium growth or if it's cobweb or another type of contamination.
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OkieFunguy
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Re: Contamination question [Re: OkieFunguy]
#22351827 - 10/08/15 08:23 PM (8 years, 3 months ago) |
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Anyone have an answer for this? The jar is still with all the other jars and I don't want to leave it in there if it is contaminated.
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cronicr



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Re: Contamination question [Re: OkieFunguy]
#22352135 - 10/08/15 09:28 PM (8 years, 3 months ago) |
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lighting sucks but it doens't look good
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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aerow.thefox
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Re: Contamination question [Re: cronicr]
#22352247 - 10/08/15 09:52 PM (8 years, 3 months ago) |
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looks bad to me. better pic might give a more definite answer. but if your others look like the one on the right then something is off. or else it'd be there with them or close to.
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RJ Tubs 202



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Re: Contamination question [Re: OkieFunguy]
#22352257 - 10/08/15 09:56 PM (8 years, 3 months ago) |
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I think it's a good bet the jar on the left is contaminated. If you look at it with a magnifying glass, if it's another type of fungus, it will probably look different in color and texture than the good one on the right.
Quote:
OkieFunguy said: I did 10 jars sterilized in a pot, and then went and bought a pressure cooker and did 12 more jars. The jars sterilized in the pot are doing way better than the pressure cooker jars. I must have done something wrong with the pressure cooker.
My guess is the difference might be due to...
The PC jars were not totally sterilized. How much time and at what temp did you cook? (I recently tried, for the first time, a hospital autoclave used for sterilizing tools, and 30 minutes was not enough for PF jars, and they all contaminated)
Is there any way water could have gotten in the PC jars during cooking? That would jack them up. Did you use foil on the lids?
I assume you used exactly the same verm formula with the exact same amounts and ratio of water/BRF/verm for both batches.
Did you use the same syringe or LC for both batches of jars? Are they the same type? There can be differences in how long different types take to take off... as much as 4-6 days.
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OkieFunguy
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I used different syringes but the same strain. 4 of the PC'ed jars have small mycelium growth so maybe some will work out. I did have foil. I PC'ed for two hours on a "meat" setting. My pressure cooker doesn't have a temperature gauge. The PC'ed jars looked darker than the ones I did in the pot, so I think they did get some water in them. Another weird thing is that I inoculated the first batch in the pot almost 3 weeks ago, and a couple of the jars have zero growth yet while 5 of them are thriving. I inoculated the PC'ed batch exactly a week after the others and I have the 4 jars with beginning mycelium. I had to throw one PC jar out yesterday due to a dark brown mold. I will go ahead and throw the jar mentioned in the OP out.
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