Home | Community | Message Board

Out-Grow.com - Mushroom Growing Kits & Supplies
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Unfolding Nature Unfolding Nature: Being in the Implicate Order   PhytoExtractum Buy Bali Kratom Powder   North Spore Injection Grain Bag   Mushroom-Hut Substrate Bags   Original Sensible Seeds Bulk Cannabis Seeds   Left Coast Kratom Buy Kratom Extract

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Offlinecvh
The adult


Registered: 09/18/15
Posts: 57
Loc: Mainland Western Europe
Last seen: 1 year, 11 months
Cloning question
    #22302785 - 09/28/15 01:05 PM (8 years, 4 months ago)

Hi all,

When reading up on cloning a question arose for me.
Is it possible to indefinitely keep cloning?

I mean, you start with one sample, place this on agar, use this then to grow.
Take a new sample, place this on agar, grow, sample,...and so on.

Or would the mycelium start degenerating over time after each generation?
If so, what's the best practice? Take spores and start again?


--------------------
:mushroom2:


Extras: Filter Print Post Top
InvisibleMunchauzen
 User Gallery

Registered: 06/22/11
Posts: 14,342
Trusted Cultivator
Re: Cloning question [Re: cvh]
    #22302805 - 09/28/15 01:10 PM (8 years, 4 months ago)

in theory, the culture would eventually weaken and die out. how long that would take, well we don't really know. some mycologists have said you can go a whole lifetime without experiencing this.


Extras: Filter Print Post Top
OfflinecronicrFacebook
 User Gallery


Registered: 08/07/11
Posts: 61,436
Loc: Van Isle Flag
Last seen: 2 years, 8 days
Trusted Cultivator
Re: Cloning question [Re: Munchauzen]
    #22302846 - 09/28/15 01:16 PM (8 years, 4 months ago)

the idea is to keep the culture young so once you clone keep your first plate orplates, the more cell division that happens the weaker your myc network will become


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


Extras: Filter Print Post Top
InvisibleMunchauzen
 User Gallery

Registered: 06/22/11
Posts: 14,342
Trusted Cultivator
Re: Cloning question [Re: cronicr]
    #22302855 - 09/28/15 01:18 PM (8 years, 4 months ago)

Quote:

cronicr said:
the idea is to keep the culture young so once you clone keep your first plate orplates, the more cell division that happens the weaker your myc network will become




:whathesaid:

when I said in theory, I should have followed with in practice, you probably don't want to test this.


Extras: Filter Print Post Top
Invisiblemicro
bunbun has a gungun
Male User Gallery


Registered: 05/09/03
Posts: 7,532
Loc: Brick City Flag
Re: Cloning question [Re: Munchauzen]
    #22302912 - 09/28/15 01:31 PM (8 years, 4 months ago)

Quote:

Munchauzen said:
in theory, the culture would eventually weaken and die out. how long that would take, well we don't really know. some mycologists have said you can go a whole lifetime without experiencing this.




Best I could find was 26 generations in a different type of fungi.


--------------------
Any research paper or book for free
(Avatar is Maxxy, a character by Mizzyam, RIP)


Extras: Filter Print Post Top
InvisiblebodhisattaMDiscordReddit
Smurf real estate agent
 User Gallery
Folding@home Statistics
Registered: 04/30/13
Posts: 61,889
Loc: Milky way
Trusted Cultivator
Re: Cloning question [Re: micro]
    #22302988 - 09/28/15 01:49 PM (8 years, 4 months ago)

worst case scenario you fruit a culture until it falls apart by cloning your clones. take a print and start over


Extras: Filter Print Post Top
OfflinePsilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
Re: Cloning question [Re: bodhisatta]
    #22303232 - 09/28/15 02:39 PM (8 years, 4 months ago)

Quote:

bodhisatta said:
worst case scenario you fruit a culture until it falls apart by cloning your clones.



:lolwut:


Extras: Filter Print Post Top
OfflineSivarted
Stranger
Registered: 08/22/15
Posts: 1,084
Last seen: 2 months, 10 days
Re: Cloning question [Re: Psilosoulful]
    #22303328 - 09/28/15 03:04 PM (8 years, 4 months ago)

A search on senescence will give plenty of info on the topic.

The short answer is that you can't do it indefinitely, no.  And there are many ways to avoid having to do so in order to keep an original isolated master culture alive, so there's no reason to clone clones of clones of clones.


Extras: Filter Print Post Top
OfflinePsilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
Re: Cloning question [Re: Sivarted]
    #22303521 - 09/28/15 03:39 PM (8 years, 4 months ago)

Thanks for the info :takingnotes:


Extras: Filter Print Post Top
Offlinemushpunx
Fungus Punk
 User Gallery


Registered: 04/20/14
Posts: 13,394
Last seen: 11 days, 13 hours
Trusted Cultivator
Re: Cloning question [Re: Psilosoulful]
    #22303696 - 09/28/15 04:15 PM (8 years, 4 months ago)

When I take a clone, I clean it up and grow out two plates.. one to put to grain and one for the fridge, labeled. If it performs well, I slant it.
If you don't know what that is its basically a test tube with agar that was cooled at an angle to give a long slant of surface area for the culture to grow on.
These can be stored for years if done right.

When you want to use the slant, you pull out a tiny piece of myc and put it to a new plate.


--------------------

Amateur Mycologists United
AMU Q&A


Extras: Filter Print Post Top
OfflineKizzle
Misanthrope
 User Gallery

Registered: 08/30/11
Posts: 9,855
Last seen: 19 hours, 11 minutes
Trusted Cultivator
Re: Cloning question [Re: mushpunx]
    #22304301 - 09/28/15 06:08 PM (8 years, 4 months ago)

Keep a master culture. Frozen cultures can survive for decades. So in theory you could grow a lifetime worth of mushrooms from a single culture just by cloning tiny bits it occasionally to replace senescent lines.


--------------------


Extras: Filter Print Post Top
Invisiblemicelio
Song of Silence
 User Gallery

Registered: 04/22/14
Posts: 1,547
Re: Cloning question [Re: cvh]
    #22304398 - 09/28/15 06:29 PM (8 years, 4 months ago)



--------------------
     


Extras: Filter Print Post Top
Offlinemushpunx
Fungus Punk
 User Gallery


Registered: 04/20/14
Posts: 13,394
Last seen: 11 days, 13 hours
Trusted Cultivator
Re: Cloning question [Re: Kizzle]
    #22304534 - 09/28/15 06:52 PM (8 years, 4 months ago)

Quote:

Kizzle said:
Keep a master culture. Frozen cultures can survive for decades. So in theory you could grow a lifetime worth of mushrooms from a single culture just by cloning tiny bits it occasionally to replace senescent lines.





Frozen like in the freezer?? Or is that like a cultivation term :lol:


--------------------

Amateur Mycologists United
AMU Q&A


Extras: Filter Print Post Top
OfflineKizzle
Misanthrope
 User Gallery

Registered: 08/30/11
Posts: 9,855
Last seen: 19 hours, 11 minutes
Trusted Cultivator
Re: Cloning question [Re: mushpunx]
    #22305024 - 09/28/15 08:32 PM (8 years, 4 months ago)

You can freeze mycelium without killing it. At least in the early stages.


--------------------


Extras: Filter Print Post Top
OfflinePussyFart
Retired Cultivation Extrodinaire
Male


Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 17 days, 21 hours
Trusted Cultivator
Re: Cloning question [Re: Kizzle]
    #22305061 - 09/28/15 08:39 PM (8 years, 4 months ago)

Refrigeration is safer and works just the same no?


Extras: Filter Print Post Top
OfflinecronicrFacebook
 User Gallery


Registered: 08/07/11
Posts: 61,436
Loc: Van Isle Flag
Last seen: 2 years, 8 days
Trusted Cultivator
Re: Cloning question [Re: PussyFart]
    #22305674 - 09/28/15 10:33 PM (8 years, 4 months ago)

freezing is also a great option...so is storing the fruit itself:hehehe:


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


Extras: Filter Print Post Top
Offlinemushpunx
Fungus Punk
 User Gallery


Registered: 04/20/14
Posts: 13,394
Last seen: 11 days, 13 hours
Trusted Cultivator
Re: Cloning question [Re: cronicr]
    #22306645 - 09/29/15 05:20 AM (8 years, 4 months ago)

Well that's pretty cool man that's the first time I've heard that!

Learning stuff !


--------------------

Amateur Mycologists United
AMU Q&A


Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Unfolding Nature Unfolding Nature: Being in the Implicate Order   PhytoExtractum Buy Bali Kratom Powder   North Spore Injection Grain Bag   Mushroom-Hut Substrate Bags   Original Sensible Seeds Bulk Cannabis Seeds   Left Coast Kratom Buy Kratom Extract


Similar ThreadsPosterViewsRepliesLast post
* Simple Cloning Question.. LucidDayDream 1,404 3 05/17/02 10:06 AM
by mew
* clone question whitesox 899 5 02/21/03 08:27 AM
by z@z.com
* HoneyTek and Tissue Cloning questions... UnclePimpy 1,303 2 02/10/02 07:31 PM
by Zen Peddler
* Reasking a cloning question, HELP MOE !!! QuitDrinking 766 2 01/03/03 10:53 AM
by Zen Peddler
* cloning (shrooms not humans), need expert opinion. QuitDrinking 2,231 5 12/30/02 06:50 AM
by Curious_George
* Cloning question *DELETED* MeetMeInEleusis 652 1 03/22/02 01:25 PM
by puscle
* Re: non-entheogenic mushroom cloning questions Anonymous 902 2 01/20/00 06:05 PM
by Anonymous
* questions, questions, and questions, oh my! thel0rax 769 5 12/10/02 05:32 PM
by MickyFinn

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
496 topic views. 22 members, 239 guests and 62 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.03 seconds spending 0.01 seconds on 14 queries.