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cvh
The adult


Registered: 09/18/15
Posts: 57
Loc: Mainland Western Europe
Last seen: 1 year, 11 months
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Cloning question
#22302785 - 09/28/15 01:05 PM (8 years, 4 months ago) |
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Hi all,
When reading up on cloning a question arose for me. Is it possible to indefinitely keep cloning?
I mean, you start with one sample, place this on agar, use this then to grow. Take a new sample, place this on agar, grow, sample,...and so on.
Or would the mycelium start degenerating over time after each generation? If so, what's the best practice? Take spores and start again?
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Munchauzen


Registered: 06/22/11
Posts: 14,342
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Re: Cloning question [Re: cvh]
#22302805 - 09/28/15 01:10 PM (8 years, 4 months ago) |
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in theory, the culture would eventually weaken and die out. how long that would take, well we don't really know. some mycologists have said you can go a whole lifetime without experiencing this.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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the idea is to keep the culture young so once you clone keep your first plate orplates, the more cell division that happens the weaker your myc network will become
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Munchauzen


Registered: 06/22/11
Posts: 14,342
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Re: Cloning question [Re: cronicr]
#22302855 - 09/28/15 01:18 PM (8 years, 4 months ago) |
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Quote:
cronicr said: the idea is to keep the culture young so once you clone keep your first plate orplates, the more cell division that happens the weaker your myc network will become

when I said in theory, I should have followed with in practice, you probably don't want to test this.
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micro
bunbun has a gungun



Registered: 05/09/03
Posts: 7,532
Loc: Brick City
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Quote:
Munchauzen said: in theory, the culture would eventually weaken and die out. how long that would take, well we don't really know. some mycologists have said you can go a whole lifetime without experiencing this.
Best I could find was 26 generations in a different type of fungi.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Cloning question [Re: micro]
#22302988 - 09/28/15 01:49 PM (8 years, 4 months ago) |
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worst case scenario you fruit a culture until it falls apart by cloning your clones. take a print and start over
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Psilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
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Quote:
bodhisatta said: worst case scenario you fruit a culture until it falls apart by cloning your clones.
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Sivarted
Stranger
Registered: 08/22/15
Posts: 1,084
Last seen: 2 months, 10 days
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A search on senescence will give plenty of info on the topic.
The short answer is that you can't do it indefinitely, no. And there are many ways to avoid having to do so in order to keep an original isolated master culture alive, so there's no reason to clone clones of clones of clones.
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Psilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
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Re: Cloning question [Re: Sivarted]
#22303521 - 09/28/15 03:39 PM (8 years, 4 months ago) |
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Thanks for the info
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mushpunx
Fungus Punk



Registered: 04/20/14
Posts: 13,394
Last seen: 11 days, 13 hours
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When I take a clone, I clean it up and grow out two plates.. one to put to grain and one for the fridge, labeled. If it performs well, I slant it. If you don't know what that is its basically a test tube with agar that was cooled at an angle to give a long slant of surface area for the culture to grow on. These can be stored for years if done right.
When you want to use the slant, you pull out a tiny piece of myc and put it to a new plate.
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 Amateur Mycologists United AMU Q&A
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Kizzle
Misanthrope


Registered: 08/30/11
Posts: 9,855
Last seen: 19 hours, 11 minutes
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Re: Cloning question [Re: mushpunx]
#22304301 - 09/28/15 06:08 PM (8 years, 4 months ago) |
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Keep a master culture. Frozen cultures can survive for decades. So in theory you could grow a lifetime worth of mushrooms from a single culture just by cloning tiny bits it occasionally to replace senescent lines.
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micelio
Song of Silence


Registered: 04/22/14
Posts: 1,547
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Re: Cloning question [Re: cvh]
#22304398 - 09/28/15 06:29 PM (8 years, 4 months ago) |
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mushpunx
Fungus Punk



Registered: 04/20/14
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Re: Cloning question [Re: Kizzle]
#22304534 - 09/28/15 06:52 PM (8 years, 4 months ago) |
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Quote:
Kizzle said: Keep a master culture. Frozen cultures can survive for decades. So in theory you could grow a lifetime worth of mushrooms from a single culture just by cloning tiny bits it occasionally to replace senescent lines.
Frozen like in the freezer?? Or is that like a cultivation term
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Kizzle
Misanthrope


Registered: 08/30/11
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Re: Cloning question [Re: mushpunx]
#22305024 - 09/28/15 08:32 PM (8 years, 4 months ago) |
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You can freeze mycelium without killing it. At least in the early stages.
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
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Re: Cloning question [Re: Kizzle]
#22305061 - 09/28/15 08:39 PM (8 years, 4 months ago) |
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Refrigeration is safer and works just the same no?
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
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Re: Cloning question [Re: PussyFart]
#22305674 - 09/28/15 10:33 PM (8 years, 4 months ago) |
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freezing is also a great option...so is storing the fruit itself
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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mushpunx
Fungus Punk



Registered: 04/20/14
Posts: 13,394
Last seen: 11 days, 13 hours
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Re: Cloning question [Re: cronicr]
#22306645 - 09/29/15 05:20 AM (8 years, 4 months ago) |
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Well that's pretty cool man that's the first time I've heard that!
Learning stuff !
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