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OfflineGreenbastard
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Registered: 09/28/15
Posts: 46
Last seen: 5 years, 9 months
Orissa India GLC>Mono-tub project!
    #22302597 - 09/28/15 12:27 PM (8 years, 4 months ago)

Hello all my fellow cultivators. Allow me to introduce myself. My name is Greenbastard (parts unknown) and I've been a LOOONG time lurker and stalker of many teks and grow logs on this outstanding website. I have previous good results with cakes ,about 7 grows in a 4 year period, averaging decent yields with golden teachers and PE. Those familiar with PE will understand my level of patience when it comes to cultivation. Also did a single monotub run Orissa 6Q spawn/50/50 that pushed out a whopping 7oz dry first flush and 1.5 dry after dunking. So basically this will be a play by play but slightly larger. I would just like to first off THANK EACH AND EVERY ONE OF YOU for this community, you guys are amazing and your level of experience and expertise is the only thing that allowed me to experience the rush of my very first pinset!

Now let's get down to the nitty gritty

I have two OI MS syringes that I plan on doing a GLC in a pint of colonized WBS then using 100ml of said mycelium water to knock up 48 (yes 48) quart jars of WBS +-2cc each. Then spawned onto  coir/verm,50/50, into a 66qt sterilize monotub.

I'm looking forward to meeting and chatting with you ladies and gentlemen and hopefully gaining experience and knowledge along the way.

Cheers


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OfflinePussyFart
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Registered: 04/08/12
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Last seen: 17 days, 19 hours
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Re: Orissa India GLC>Mono-tub project! [Re: Greenbastard]
    #22302608 - 09/28/15 12:30 PM (8 years, 4 months ago)

I would skip the GLC, and just G2G them out....GLC is just another vector for contams....


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OfflineGreenbastard
DECENT!

Registered: 09/28/15
Posts: 46
Last seen: 5 years, 9 months
Re: Orissa India GLC>Mono-tub project! [Re: PussyFart]
    #22302616 - 09/28/15 12:33 PM (8 years, 4 months ago)

Yea I thought so too. I haven't got around to make my SAB yet.


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OfflineGreenbastard
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Registered: 09/28/15
Posts: 46
Last seen: 5 years, 9 months
Re: Orissa India GLC>Mono-tub project! [Re: PussyFart]
    #22302677 - 09/28/15 12:51 PM (8 years, 4 months ago)

Question, if I'm injecting sterile water into a colonized grain jar to make mycelium water where is the contam risk if standard disinfecting precautions are taken into consideration?


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OfflineGreenbastard
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Registered: 09/28/15
Posts: 46
Last seen: 5 years, 9 months
Re: Orissa India GLC>Mono-tub project! [Re: Greenbastard]
    #22303495 - 09/28/15 03:34 PM (8 years, 4 months ago)

Anyone?


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OfflinePussyFart
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Re: Orissa India GLC>Mono-tub project! [Re: Greenbastard]
    #22303544 - 09/28/15 03:44 PM (8 years, 4 months ago)

Rules Thread-READ BEFORE POSTING

Focus on the 4th one....


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OfflinePussyFart
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Re: Orissa India GLC>Mono-tub project! [Re: PussyFart]
    #22303558 - 09/28/15 03:45 PM (8 years, 4 months ago)

You are adding extra, unneeded steps into the process... Just pour the grains from one jar to another.


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Offlinemushpunx
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Registered: 04/20/14
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Re: Orissa India GLC>Mono-tub project! [Re: Greenbastard]
    #22303614 - 09/28/15 04:01 PM (8 years, 4 months ago)

Quote:

Greenbastard said:
Question, if I'm injecting sterile water into a colonized grain jar to make mycelium water where is the contam risk if standard disinfecting precautions are taken into consideration?




You really shouldn't even be doing this work without a still air box anyways man.

If you inoculated the grain jar with spore solution you really dont have any way to know if it isn't contaminated with mold or bacteria.. mold won't usually show u ntill you fruit your tubs.

All liquid culture should begin with agar. Actually amy inoculation (brf excluded, its a bit more forgiving IMO) should begin with agar, untill you get your culture to a point where you are sure it is clean.

Build a still air box, learn to use it properly. Its a 6 dollar tote with 2 arm holes in it..

Learn to get a clean culture on agar, then inoculate your master jars with that and grain to grain transfer. Its a mycology staple.

For this jar, grain to grain transfer in a still air box and hope for the best :thumbup:


--------------------

Amateur Mycologists United
AMU Q&A


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OfflinePussyFart
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Re: Orissa India GLC>Mono-tub project! [Re: mushpunx]
    #22303629 - 09/28/15 04:03 PM (8 years, 4 months ago)

Quote:

mushpunx said:
Build a still air box, learn to use it properly. Its a 6 dollar tote with 2 arm holes in it..



$6 for a small, cramped one....

I would spend $15-$20 on the biggest clear tub you can find, and cut the arm holes a good distance apart from one another, as to be comfortable while you're working smart.


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Offlinemushpunx
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Re: Orissa India GLC>Mono-tub project! [Re: PussyFart]
    #22303656 - 09/28/15 04:08 PM (8 years, 4 months ago)

Yea, right on by all means do it right and make a proper one! My point was more that they are so easy to make and inexpensive that there's not much excuse to let your work suffer without one


--------------------

Amateur Mycologists United
AMU Q&A


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OfflineGreenbastard
DECENT!

Registered: 09/28/15
Posts: 46
Last seen: 5 years, 9 months
Re: Orissa India GLC>Mono-tub project! [Re: PussyFart]
    #22303665 - 09/28/15 04:10 PM (8 years, 4 months ago)

Thanks for the input guys.


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OfflinePussyFart
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Re: Orissa India GLC>Mono-tub project! [Re: mushpunx]
    #22303668 - 09/28/15 04:10 PM (8 years, 4 months ago)

:asianofapproval:


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