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OfflineCrentistTheBensis
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Re: Shmuv box consensus [Re: bodhisatta]
    #22295753 - 09/26/15 08:34 PM (8 years, 4 months ago)

Quote:

bodhisatta said:
pastywhyte's easy agar tek is the one



I just checked it out. I think everything is going to be just fine.:laugh::thumbup::grin:


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Offlinemushpunx
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Re: Shmuv box consensus [Re: CrentistTheBensis]
    #22295901 - 09/26/15 09:04 PM (8 years, 4 months ago)

Goodie Gumdrops! I think thats a great place for you to start man. Update us!


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OfflineCrentistTheBensis
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Re: Shmuv box consensus [Re: mushpunx]
    #22295924 - 09/26/15 09:10 PM (8 years, 4 months ago)

I just ordered what I needed for the easy agar tek, so in a few days I'll do a whole write up with picturesand post it here, just for shits.


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Invisiblemicelio
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Re: Shmuv box consensus [Re: CrentistTheBensis]
    #22296157 - 09/26/15 09:53 PM (8 years, 4 months ago)

I'm a believer in using your clean room for cultures agar work only.
No growing or mixing substrate in the clean room.
Keep the clean room as small as possible.
clean clothing.
No carpet or rugs in the clean room.
No fans or air movement.
Always wash your arms and hand before entering your clean room.
The bottom of your shoes are a great carrier of contaminates.

There's approximately one million particles in a cubic foot of outside air and one half of all those particles are live contaminates.( John Holliday Aloha medicinal, with his laser counter)

:cookiemonster:


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Offlinemushpunx
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Re: Shmuv box consensus [Re: micelio]
    #22296234 - 09/26/15 10:07 PM (8 years, 4 months ago)

Yea man I think this is where a lot of my problems come from. I only do culture/spawn work in that room but its certainly not as clean as I'd like to be able to keep it


But if you keep the table your flow hood is on clean, should it matter what the rest of the room is like if you work in the stream of the flow hood? I mean isnt that the point of a hood? Excluding your clothing, gloves/sleeves, wiping your work and workspoace w ISO etc

And for SABs I would think that goes double?


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InvisiblePastywhyteMDiscord
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Re: Shmuv box consensus [Re: mushpunx]
    #22296344 - 09/26/15 10:33 PM (8 years, 4 months ago)

Actually from what I have heard a FH needs even more attention to the overall sporeload of the room, but I could use an SAB in a room with 50 loaves of trich ridden bread with success. A FH is just about ergonomics and is by no means more forgiving AFAIK.


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Invisiblemicelio
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Re: Shmuv box consensus [Re: mushpunx]
    #22296347 - 09/26/15 10:33 PM (8 years, 4 months ago)

Quote:

mushpunx said:
Yea man I think this is where a lot of my problems come from. I only do culture/spawn work in that room but its certainly not as clean as I'd like to be able to keep it


But if you keep the table your flow hood is on clean, should it matter what the rest of the room is like if you work in the stream of the flow hood? I mean isnt that the point of a hood? Excluding your clothing, gloves/sleeves, wiping your work and workspoace w ISO etc

And for SABs I would think that goes double?



Your right. Working in the airstream of a flow hood makes all the difference in the world. All my plates are super clean. I haven't seen green in along time.. I still like using a check list though, just to keep myself from getting sloppy...


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InvisiblebodhisattaMDiscordReddit
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Re: Shmuv box consensus [Re: Pastywhyte]
    #22296360 - 09/26/15 10:36 PM (8 years, 4 months ago)

yep a FH basically needs a dedicated room with surfaces you can wipe down. if you're using it in a room with carpet that's pretty stupid if you ask me. not only that but your sterile technique has to be more spot on too.

still air box you could do that in the mcdonalds play place.


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InvisiblePastywhyteMDiscord
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Re: Shmuv box consensus [Re: bodhisatta]
    #22296366 - 09/26/15 10:36 PM (8 years, 4 months ago)

Quote:

bodhisatta said:
the mcdonalds play place.




:puke:


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OfflineMachiavelliavore
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Re: Shmuv box consensus [Re: bodhisatta]
    #22297033 - 09/27/15 04:31 AM (8 years, 4 months ago)

Quote:

bodhisatta said:
at least with no pour you can re-use. I use pour plates because I get petri dishes free or next to free. if I have to pay it's like 2.50 a sleeve of 20.

it sounds stupid but try rehearsal. if your hands ever go above anything sterile = wrong

there's some good home made vids floating around the shroomery with SAB work too




Seriously, don't put your damn hands above what you're working on.  I used to be focused on a lot of other things, like covering my arms to the elbows with alcohol every time I pulled my arms out of the airbox.  Now I pretty much focus on my hand positioning and trying to be relaxed and smoothe about what I'm doing.  Stopped trying to be speedy and overly santizing.

You can get some nopour plates nearly that cheap from dollartree (5/$1.)  Place is magic for pp5 shit :smile:


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I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


Edited by Machiavelliavore (09/27/15 04:32 AM)


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OfflineCrentistTheBensis
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Re: Shmuv box consensus [Re: Machiavelliavore]
    #22297730 - 09/27/15 10:21 AM (8 years, 4 months ago)

Quote:

Machiavelliavore said:
Quote:

bodhisatta said:
at least with no pour you can re-use. I use pour plates because I get petri dishes free or next to free. if I have to pay it's like 2.50 a sleeve of 20.

it sounds stupid but try rehearsal. if your hands ever go above anything sterile = wrong

there's some good home made vids floating around the shroomery with SAB work too




Seriously, don't put your damn hands above what you're working on.  I used to be focused on a lot of other things, like covering my arms to the elbows with alcohol every time I pulled my arms out of the airbox.  Now I pretty much focus on my hand positioning and trying to be relaxed and smoothe about what I'm doing.  Stopped trying to be speedy and overly santizing.

You can get some nopour plates nearly that cheap from dollartree (5/$1.)  Place is magic for pp5 shit :smile:



I actually just bought some glad mini rounds, had to order micropre, but i have a really good feeling about next time.


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Offlinemushpunx
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Re: Shmuv box consensus [Re: CrentistTheBensis]
    #22298028 - 09/27/15 11:25 AM (8 years, 4 months ago)

e]bodhisatta said:
yep a FH basically needs a dedicated room with surfaces you can wipe down. if you're using it in a room with carpet that's pretty stupid if you ask me. not only that but your sterile technique has to be more spot on too.

still air box you could do that in the mcdonalds play place.





Thats what I was saying, you should be able to use a still air box anywhere as long as you wash your arms up

But while I got you guys on it

I hear conflicting information about the room a flow hood goes in though. I agree you should put a flow hood on a table that can be wiped clean before every use. You should hit the face with Lysol and run the hood for a while before you work.

  But I always thought that you should be alright as long as you wipe your work down before putting it on the table, work close to the hood, in the area of laminar flow it provides?

When you guys are talking about a dedicated room, I understand you probably don't want a nasty basement.. or yea a carpeted room (though I have an RR quote of him saying he used to work his flowhood in a bedroom with carpets that his dog slept in :lol: ) but do you really have to go beyond keeping the room reasonably clean, like swept and dusted?

I love my flow hood. Its awesome. But I struggle with more contam problems than I should hahaha. I was gunna try switching back to SAB for a minute and see if it makes much of a difference

My agar work in front of the hood is beautiful. I never see bacteria or mold colonies. But past that trich finds its way in


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OfflineKizzle
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Re: Shmuv box consensus [Re: mushpunx]
    #22298930 - 09/27/15 02:41 PM (8 years, 4 months ago)

Quote:

When you guys are talking about a dedicated room, I understand you probably don't want a nasty basement.. or yea a carpeted room (though I have an RR quote of him saying he used to work his flowhood in a bedroom with carpets that his dog slept in :lol: ) but do you really have to go beyond keeping the room reasonably clean, like swept and dusted?



I think in any case it's going mainly to come down to sterile technique but if there's dust and crap around it'll certainly make it easier to contaminate your gloves and the stuff your working before it's placed in the sterile field which makes any mistakes more likely to result in contamination.


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Invisiblemicelio
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Registered: 04/22/14
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Re: Shmuv box consensus [Re: Kizzle]
    #22299003 - 09/27/15 03:02 PM (8 years, 4 months ago)

Quote:

Kizzle said:
Quote:

When you guys are talking about a dedicated room, I understand you probably don't want a nasty basement.. or yea a carpeted room (though I have an RR quote of him saying he used to work his flowhood in a bedroom with carpets that his dog slept in :lol: ) but do you really have to go beyond keeping the room reasonably clean, like swept and dusted?



I think in any case it's going mainly to come down to sterile technique but if there's dust and crap around it'll certainly make it easier to contaminate your gloves and the stuff your working before it's placed in the sterile field which makes any mistakes more likely to result in contamination.



Well said..:thumbup:

I have gotten in the habit of buying cheap sandwich bags by the hundreds. My plate come out of the pc and directly into a sandwich bag, then into a plastic shoe boxes, just for that double protection against dust and contaminates floating around.


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Offlinemushpunx
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Re: Shmuv box consensus [Re: Kizzle]
    #22299536 - 09/27/15 05:01 PM (8 years, 4 months ago)

Quote:

Kizzle said:
Quote:

When you guys are talking about a dedicated room, I understand you probably don't want a nasty basement.. or yea a carpeted room (though I have an RR quote of him saying he used to work his flowhood in a bedroom with carpets that his dog slept in :lol: ) but do you really have to go beyond keeping the room reasonably clean, like swept and dusted?



I think in any case it's going mainly to come down to sterile technique but if there's dust and crap around it'll certainly make it easier to contaminate your gloves and the stuff your working before it's placed in the sterile field which makes any mistakes more likely to result in contamination.





Ah, right on man that makes sense. From what I understand (at least w mold like trich) its less floating around in the air and more getting picked up on things yea?

I get what you guys mean now. Thx for clarifying


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