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CrentistTheBensis
Strangest


Registered: 07/15/15
Posts: 63
Loc: The Baltic sea
Last seen: 3 years, 4 months
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Shmuv box consensus
#22294687 - 09/26/15 05:19 PM (8 years, 4 months ago) |
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Hey everybody,
So I'm having terrible luck with agar work in my still air box. My sterile procedure is pretty over the top, but I'm still seeing a terrible success rate. I cannot afford to buy or build a flow hood,but I recently saw a couple threads involving the use of a shmuv box. My question isn't nessisarily how it works, but rather how WELL it works. If anyone uses one regularly and has any input I would greatly appreciate it!
-------------------- I'm the grains of the operation.
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Dionili
Second Rate Mycologist



Registered: 08/18/09
Posts: 2,194
Loc: Between a Rock and a Hard...
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Describe your sterile procedure your lacking some where
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jbaby007
Badass



Registered: 02/28/15
Posts: 1,026
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Check out the Let's Grow Mushrooms videos. RR goes over his sterile technique which is pretty much the same even in a SAB. It doesn't matter if you use a flowhood, a SAB, or a cardboard box, if your technique sucks and you don't know what to look for, your success rates are going to suck.
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CrentistTheBensis
Strangest


Registered: 07/15/15
Posts: 63
Loc: The Baltic sea
Last seen: 3 years, 4 months
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Re: Shmuv box consensus [Re: Dionili]
#22294761 - 09/26/15 05:35 PM (8 years, 4 months ago) |
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So I have a 105 quart tote with 2 roughly 4" holes drilled in ithe, anyways this step by step pretty much what I do: -shower -brush teeth -spray sab with lysol and a bleach solution -wash and dry a large towel -soak it in soapy water -lay it on my work surface -put on gloves, sleeves, mask, etc -wipe down items with rubbing alc -place them on towel -spray and wipe down the box again -fold edges of towel over the box and tape them (The box has no bottom, the wet towel is the bottom per spitball sab tek) -uncover holes and put in my arms - wipe down all the stuff inside with alc -proceed to do what I'm doing -in between each plate or jar or whatever I wipe everything down again -seal everything up -done.
I'm sure there is something at some point that I'm doing wrong, but nobody has perfect sterile procedure. I'm assuming that a shmuv would at least be a little bit of a leg up. Am I correct in that assumtion?
-------------------- I'm the grains of the operation.
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CrentistTheBensis
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Registered: 07/15/15
Posts: 63
Loc: The Baltic sea
Last seen: 3 years, 4 months
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Re: Shmuv box consensus [Re: jbaby007]
#22294790 - 09/26/15 05:41 PM (8 years, 4 months ago) |
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Quote:
jbaby007 said: Check out the Let's Grow Mushrooms videos. RR goes over his sterile technique which is pretty much the same even in a SAB. It doesn't matter if you use a flowhood, a SAB, or a cardboard box, if your technique sucks and you don't know what to look for, your success rates are going to suck. 
I have the video series, it is most dope. It's cool seeing RR actally do the stuff he posts about, sorta like putting a face to a name.
-------------------- I'm the grains of the operation.
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jbaby007
Badass



Registered: 02/28/15
Posts: 1,026
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I've never even heard of a shmuv box until this thread so I looked it up. It seems a hell of a lot more complicated than what is necessary. A simple plastic sterilite tub or something similar with some arm holes cut out is all you really need. The SAB doesn't need to be sprayed with lysol or bleach. It's not a sanitary box, it's only used to keep the air still while you are inoculating/g2g/agar.
You can just spray soapy water or even just water on the walls of your SAB that way any loose dust/particles can cling to it while you work on whatever it is you're doing. Make sure you aren't putting anything (besides your scalpel) over your open media.
Never wipe down with iso after flame sterilizing your needle/scalpel, it defeats the purpose. When you're working with agar, remember that you can transfer healthy mycelium away from any contams to a new plate too. That's the great thing about agar.
Check out these videos too, they might help.
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Edited by jbaby007 (09/26/15 05:58 PM)
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CrentistTheBensis
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Registered: 07/15/15
Posts: 63
Loc: The Baltic sea
Last seen: 3 years, 4 months
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Re: Shmuv box consensus [Re: jbaby007]
#22294953 - 09/26/15 06:19 PM (8 years, 4 months ago) |
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Thanks man! I think I'll just keep trying it how I'm doing and make any improvements I can think of. Maybe one day I'll make a change to a flow hood or a shmuv box, but I don't think a shmuv will just fix all my problems though. Wishful thinking.
-------------------- I'm the grains of the operation.
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bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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If a SAB doesn't work for you, you are the problem. 100s have used it with great success. Techniques are far more important than the tool be it a FH SAB or shumv
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mushpunx
Fungus Punk



Registered: 04/20/14
Posts: 13,394
Last seen: 11 days, 9 hours
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There are a few folks on here that are much more experienced growers than I do that swear by them. I don't know too much about them myself though, never used one.
Honestly I would stick with the Still air box. Its definitely not the problem, it has to be an error in your tecnique somewhere. If you are having trouble with agar work in SAB you will have trouble in front of a flow hood too.
The only real edge a flow hood provides is elbow room
What exactly are the troubles you are having with your agar work dude? Whats going wrong exactly?
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 Amateur Mycologists United AMU Q&A
Edited by mushpunx (09/26/15 06:46 PM)
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CrentistTheBensis
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Posts: 63
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Last seen: 3 years, 4 months
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Re: Shmuv box consensus [Re: mushpunx]
#22295119 - 09/26/15 07:00 PM (8 years, 4 months ago) |
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I can't pour a plate or clone tissue really, a zero percent success rate I suppose. Also, when I swipe spores or put tissue on a plate, Trich and bacteria grows fine, but I never see any myc. I assume it's because the myc never has a chance.
-------------------- I'm the grains of the operation.
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blackdust


Registered: 02/28/09
Posts: 8,327
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Re: Shmuv box consensus [Re: Dionili]
#22295126 - 09/26/15 07:01 PM (8 years, 4 months ago) |
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Quote:
Dionili said: Describe your sterile procedure your lacking some where
 havnt seen you in a while bro
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mushpunx
Fungus Punk



Registered: 04/20/14
Posts: 13,394
Last seen: 11 days, 9 hours
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Re: Shmuv box consensus [Re: blackdust]
#22295209 - 09/26/15 07:12 PM (8 years, 4 months ago) |
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Are you pouring into disposable plates yea? Have you ever given your plates a couple days wrapped up after you've poured them, to see if they were poured clean? I
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 Amateur Mycologists United AMU Q&A
Edited by mushpunx (09/26/15 07:15 PM)
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CrentistTheBensis
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Registered: 07/15/15
Posts: 63
Loc: The Baltic sea
Last seen: 3 years, 4 months
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Re: Shmuv box consensus [Re: mushpunx]
#22295370 - 09/26/15 07:30 PM (8 years, 4 months ago) |
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Yes to both things. I use pre sterilized plates straight from the sleeve,and they all contamed. When I first got started i bought pre poured plates that I later contamed. If you guys ever need some Trich I'm a fucking master with that shit. I grow it without even trying!
-------------------- I'm the grains of the operation.
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blackdust


Registered: 02/28/09
Posts: 8,327
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Re: Shmuv box consensus [Re: mushpunx]
#22295381 - 09/26/15 07:33 PM (8 years, 4 months ago) |
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yeah reccomend practice states to test sterile method by allowing vessels to sit to check for contam isuuses
do this for cakes, grains, agar, etc
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mushpunx
Fungus Punk



Registered: 04/20/14
Posts: 13,394
Last seen: 11 days, 9 hours
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Dude if you saw how green some of my tubs get haha.. like Oscar the grouch in his trash can
So youre plates are getting contaminated during the pour? The way that usually happens is if your fingers slip under the lids or touch the inside of the plate, even if your fingers don't touch anything they can drop contams into your work.
Is your still air box tall enough to let your work comfortably? Are you turning off anything that would stir up air currents in the room?
I sugguest maybe you try no pour plates for a little while, do some work and see if you can't get better results
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 Amateur Mycologists United AMU Q&A
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CrentistTheBensis
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Registered: 07/15/15
Posts: 63
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Last seen: 3 years, 4 months
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Re: Shmuv box consensus [Re: mushpunx]
#22295546 - 09/26/15 07:54 PM (8 years, 4 months ago) |
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First of all, thanks man. I really appreciate the replies and the support, you troubleshooting this with me means alot and has inspired me to continue with trial and (mostly) error. Anyways, I have a small room that's pretty ideal with no air currents moving through it. I'm going to get more no pour plates and get that down first, although they are about 2X more expensive, I'd probably end up spending 4X that amount with all the agar and plates I would contam. Some day when I have a fridge full of insanely dope master slants this will all be well worth it.
-------------------- I'm the grains of the operation.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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at least with no pour you can re-use. I use pour plates because I get petri dishes free or next to free. if I have to pay it's like 2.50 a sleeve of 20.
it sounds stupid but try rehearsal. if your hands ever go above anything sterile = wrong
there's some good home made vids floating around the shroomery with SAB work too
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CrentistTheBensis
Strangest


Registered: 07/15/15
Posts: 63
Loc: The Baltic sea
Last seen: 3 years, 4 months
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Quote:
bodhisatta said: at least with no pour you can re-use. I use pour plates because I get petri dishes free or next to free. if I have to pay it's like 2.50 a sleeve of 20.
it sounds stupid but try rehearsal. if your hands ever go above anything sterile = wrong
there's some good home made vids floating around the shroomery with SAB work too
I got pre poured and no pour mixed up. I just looked up a no pour tek and that looks like a great way of doing things.
-------------------- I'm the grains of the operation.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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pastywhyte's easy agar tek is the one
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Darkhome
•Shaman•N•Training•



Registered: 07/10/15
Posts: 517
Loc: United States
Last seen: 7 years, 1 month
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Quote:
bodhisatta said: pastywhyte's easy agar tek is the one
He's 100% right!!!
-------------------- “The shaman is not merely a sick man, or a madman; he is a sick man who has healed himself.” ~Terence McKenna~ "NOTHING"...I post or say is "TRUE"...Any pictures are taken from the Web and any conversation is just research for a fictional character. That being said; He is constantly seeking knowledge and evolving.
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