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OfflineAmerican
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Registered: 09/25/15
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What are odds of isolating a fruiting strain?
    #22291117 - 09/25/15 11:53 PM (8 years, 4 months ago)

Hi guys!

This is my first post, long time lurker. This is not my first go around. I have some knowledge, but was looking to find out some specifics about strain isolation.

I know that depending on the species, a random spore has a 1/2, or 1/4 chance of being genetically compatible with another spore of the same mushroom. Stamets refers to "tetrapolar" and "unifactorial and bifactorial" mushroom types.

Can someone explain this?

Does that mean that any small piece of mycelium has a 1/2 or 1/4 chance of being a fruitable strain?


Let's say you have a Multi-Spore syringe, and inoculate grain or an agar plate.

The mycelium forms, and you take a small bit (.5cm triangular wedge), and put that into a Liquid Culture or turn it into a master grain jar.

What are the odds that:

1. you have "isolated" anything at all?

2. the resultant LC/Master will be a fruiting strain?



Thanks in advance for reading, and responding. Any help is appreciated, as long as it isn't ad rectum:biggrin:


Edited by American (09/25/15 11:55 PM)


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Invisiblemicro
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Re: What are odds of isolating a fruiting strain? [Re: American]
    #22291435 - 09/26/15 01:10 AM (8 years, 4 months ago)

Well, if you do it from multiple spores I think your chances of isolating a monokaryotic section is pretty slim. There are a lot of spores and it will form dikaryotic mycelium. But yeah, if it were just from one spore it would be a monokaryot and would not be able to fruit.

In practice, this should be very rare.


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OfflineAmerican
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Re: What are odds of isolating a fruiting strain? [Re: micro]
    #22291740 - 09/26/15 03:26 AM (8 years, 4 months ago)

Thanks for the quick reply! That makes sense. if I could pick your brain a bit further:

If one were to select for fast, rhizomorphic growth...

one SHOULD have a fast, rhizomorphic strain that is VERY LIKELY to fruit.

regardless of:

whether or not a single strain is isolated?


Edited by American (09/26/15 03:27 AM)


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Invisiblemicro
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Re: What are odds of isolating a fruiting strain? [Re: American]
    #22291962 - 09/26/15 06:28 AM (8 years, 4 months ago)

Quote:

American said:
If one were to select for fast, rhizomorphic growth...

one SHOULD have a fast, rhizomorphic strain that is VERY LIKELY to fruit.

regardless of:

whether or not a single strain is isolated?




well, it wouldn't necessarily be the strain but rhizomorphic will grow faster

if these are p cubensis it should fruit regardless

but rhizomorphic vs. tomentose just has to do with that section of hyphae

it could be one genetically identical organism and still have both types of growth


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InvisiblebodhisattaMDiscordReddit
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Re: What are odds of isolating a fruiting strain? [Re: micro]
    #22292999 - 09/26/15 11:24 AM (8 years, 4 months ago)

Forget about rhizomorphic. It's largely dependent on agar formula(nutritional density). Worry about healthy growth, every noob gets way too concerned with rhizo which really means shit.

You won't get any monokaryotic mycelium, its quite the endeavor to go about getting isolated monokaryotic growth. To do it they dilute spore syringes 1:100 or more and streak a single drop out on a plate and try to catch mono before it touches another mono and becomes dikaryotic (if compatible 25% chance)

If you cut the nutrients in agar recipe down by 25-50% you'll have rhizo growth every time. It really doesn't matter just get clean growth.

If you put a single drop of spore syringe on a dish there's 1000s of spores you'll end up with 100s of strains.


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Invisiblemicro
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Re: What are odds of isolating a fruiting strain? [Re: bodhisatta]
    #22293971 - 09/26/15 02:42 PM (8 years, 4 months ago)

true

tbh, i dont know why that was ever recommended

i mean, i'm sure it grows faster but it is the same mycelium

it isn't necessarily going to grow the same way when you change media


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OfflineAmerican
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Re: What are odds of isolating a fruiting strain? [Re: micro]
    #22296492 - 09/26/15 11:15 PM (8 years, 4 months ago)

This has been very helpful. Thanks, Micro and Bodhisatta.


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