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OfflineBL4Z3D247
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First time grower and have a contamination concern.
    #22289692 - 09/25/15 06:55 PM (8 years, 4 months ago)

Hello fellow shoomers, I'm currently doing my first grow. I did all the prepping of the substrate(2:1:1 PK Tek), sterilization and inoculation. I made 5 jars, they're on week 2 and I noticed some green mold on one of them and upon further investigation I saw a crack where the green mold formed(already dumped it). I started looking at the others and the one next to the cracked one had a little green spot near the top layer of the substrate(also dumped). Now I have 3 left, 2 in which I believe are doing very well. The 3rd one is the one in question because what I think might be mycelium looks a little more faint than the other 2 jars. So I've come for some advice as to if this is a mold or myc. I'd hate to dump another jar when it might not be necessary. I apologize if the pics are bad I used my cellphone. Thanks in advance.



Edited by BL4Z3D247 (09/25/15 07:07 PM)


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InvisibleTheEaglesGift
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Registered: 04/10/11
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Re: First time grower and have a contamination concern. [Re: BL4Z3D247]
    #22290957 - 09/25/15 11:18 PM (8 years, 4 months ago)

Looks like mold, but like you said, the pictures aren't the best.

Take a picture of all 3 of your jars together, it'll help show any contrast between the growths. If this one jars mycelium looks truly different in appearance than the rest, then it's probably mold.

Don't toss til you get a 100 percent confirmation though.


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OfflineBL4Z3D247
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Re: First time grower and have a contamination concern. [Re: TheEaglesGift]
    #22291862 - 09/26/15 05:06 AM (8 years, 4 months ago)

Here is what my healthy jars look like.



Also the jar in question, the growth is right where the inoculation holes are if that might make any difference. The growth is more transparent looking which is why it's hard to see in the pics. I used a magnifying glass and the growth looks very similar to myc(stringy like) but it doesn't have a brilliant white color like my other two jars.


Edited by BL4Z3D247 (09/26/15 05:17 AM)


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InvisibleTheEaglesGift
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Re: First time grower and have a contamination concern. [Re: BL4Z3D247]
    #22293976 - 09/26/15 02:43 PM (8 years, 4 months ago)

Give it some more time.


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OfflineTronix
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Re: First time grower and have a contamination concern. [Re: TheEaglesGift]
    #22294003 - 09/26/15 02:52 PM (8 years, 4 months ago)

I think this is where the myc is growing toward the glass and not down it I've had several jars look like this it's fine just let it go


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OfflineBL4Z3D247
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Re: First time grower and have a contamination concern. [Re: Tronix]
    #22294515 - 09/26/15 04:36 PM (8 years, 4 months ago)

OK, thanks guys. Yeah, I thought it was myc but it didn't look as bright white as my other jars but has the "stringy" look to it that myc has. I moved it away from the other jars just to be safe. Glad I'm not going to have to dump another jar though.


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OfflineBL4Z3D247
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Re: First time grower and have a contamination concern. [Re: BL4Z3D247]
    #22297255 - 09/27/15 07:38 AM (8 years, 4 months ago)

OK, a little update as I wasn't home for a day. I came home this morning and took a peek at the jars. From afar I can see the jar in question looked more white than a day or two ago. But upon looking closer I saw little orangish droplets where the myc is growing on the side of the jar. I thinks it might be metabolites or bacteria. What do you guys think?



Also, I apologize for sounding paranoid. It's just my first grow and I'm trying to learn from my mistakes(if I made any) for future grows. I did a lot of research and took the proper precautions, I'm just unsure of a lot as far as contaminats go and don't want to ruin the rest of my jars if it's not what I think it might be and could potentially contaminate my grow area. Thanks!


Edited by BL4Z3D247 (09/27/15 09:11 AM)


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InvisibleJacobStorm
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Re: First time grower and have a contamination concern. [Re: BL4Z3D247]
    #22299593 - 09/27/15 05:16 PM (8 years, 4 months ago)

What was your inoculation procedure dude? Whats your incubation temp?


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OfflineBL4Z3D247
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Re: First time grower and have a contamination concern. [Re: JacobStorm]
    #22300501 - 09/27/15 08:54 PM (8 years, 4 months ago)

Quote:

JacobStorm said:
What was your inoculation procedure dude? Whats your incubation temp?



I kept the jars in the pot over night, cleaned the area I was going to use, I cleaned my glove box, sprayed the room with Lysol, put on gloves, wiped gloves with rubbing alcohol, cleaned the inside of the glove box and gave it a quick spray of Lysol, wipped the bag of syringes with alcohol and placed them in the glove box, placed jars from the pot to glove box, shook syringe(from sponsored vendor) and put in the needle, wiped needle with alcohol, flame sterilized the needle with a lighter torch, inserted and put .25cc in each hole, wiped needle, torched needle, ect, ect, until I was done.

I've kept them in my room on a shelf since. The temperature has been between 70-75. I have them when the room light reaches but isn't directly on them.


Edited by BL4Z3D247 (10/24/15 11:06 AM)


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Invisiblemicro
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Re: First time grower and have a contamination concern. [Re: BL4Z3D247]
    #22301222 - 09/28/15 01:56 AM (8 years, 4 months ago)

i say metabolites

it's a liquid

the one on the right looks fine, the one on the left probably too

the myc looks sparse but it is forming the same orange stuff


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OfflineBL4Z3D247
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Re: First time grower and have a contamination concern. [Re: micro]
    #22302134 - 09/28/15 10:29 AM (8 years, 4 months ago)

Quote:

micro said:
i say metabolites

it's a liquid

the one on the right looks fine, the one on the left probably too

the myc looks sparse but it is forming the same orange stuff



Yeah, it's a liquid so I knew it wasn't mold or anything.

OK, cool. I'm glad they both look fine. I probably handled them too much which I'll avoid doing in the future. Ah, the woes of being a noob. Lol.

Thanks for your response. I thought a month of reading up on the process was enough. Seems there's still lots to absorb.

micro, since I have your attention, I saw in another thread you commented in that if using a SGFC you don't need to fan since it has adequate FAE, correct? I've been doing research all over the internet and I must be stumbling onto misinformatiin/outdated material. I have some time before I birth anything so I'm just trying to get my ducks in a row. Thanks again.


Edited by BL4Z3D247 (09/28/15 10:31 AM)


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Invisiblemicro
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Re: First time grower and have a contamination concern. [Re: BL4Z3D247]
    #22302301 - 09/28/15 11:12 AM (8 years, 4 months ago)

If you are using filters for air exchange then no, fanning it would be redundant.


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InvisibleJacobStorm
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Re: First time grower and have a contamination concern. [Re: micro]
    #22303171 - 09/28/15 02:28 PM (8 years, 4 months ago)

Quote:

micro said:
If you are using filters for air exchange then no, fanning it would be redundant.




Wait, Filters in a SGFC too avoid fanning?

We don't fan for FEA. That's what all the holes in SGFC are for. We fan to encourage water evaporation which is one of a couple "pinning triggers"

I must be confused.


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Ethnobotanical garden forum

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azur said: If you like 6th grade results, then 6th grade projects are great.


Edited by JacobStorm (09/28/15 02:36 PM)


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Invisiblemicro
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Re: First time grower and have a contamination concern. [Re: JacobStorm]
    #22303228 - 09/28/15 02:39 PM (8 years, 4 months ago)

I was under the impression FAE stood for filtered air exchange...

I also knew there was a chance I'd FCK the TLA's and that's why I spelt it out.

Also, who is "we?"

I feel like I walked into The Borg :V


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Any research paper or book for free
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InvisibleJacobStorm
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Re: First time grower and have a contamination concern. [Re: micro]
    #22303234 - 09/28/15 02:40 PM (8 years, 4 months ago)

Okay so see I thought it meant FRESH air exchange. lmao.


--------------------
Rogger Rabbits Mushroom growing videos

Ethnobotanical garden forum

Inocuole tea TEK

azur said: If you like 6th grade results, then 6th grade projects are great.


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Invisiblemicro
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Re: First time grower and have a contamination concern. [Re: JacobStorm]
    #22303240 - 09/28/15 02:41 PM (8 years, 4 months ago)

You're probably right :shrug:


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InvisibleJacobStorm
psychedelic cartel
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Registered: 07/21/15
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Re: First time grower and have a contamination concern. [Re: JacobStorm]
    #22303243 - 09/28/15 02:42 PM (8 years, 4 months ago)

Oh and by "we" I meant anyone using a SGFC.

LIke I said Confused...

Lol well which is it?!
I'd like to know if I should stop fanning my SGFC lol.


--------------------
Rogger Rabbits Mushroom growing videos

Ethnobotanical garden forum

Inocuole tea TEK

azur said: If you like 6th grade results, then 6th grade projects are great.


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Invisiblemicro
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Re: First time grower and have a contamination concern. [Re: JacobStorm]
    #22303298 - 09/28/15 02:56 PM (8 years, 4 months ago)

OBGYN

I'm sure you could come up with something...

Closed box, filters (this may be hard if it is thick plastic; you might have to melt it with a metal cylindrical object like a can, or use power tools if you have them) and possibly a (clean) fan, i.e. a power supply fan, behind the filter.

There are more clever ways to do it. When I was working in infectious disease research, we had to give a sow a sterile C-Section and put the kids in microbial isolation units. It was really just a bunch of plastic heat sealed together, and a heater and air compressor blowing hot air into the bubble. There were gloves attached to it with those metal ring clams with a screw. I assume it used HEPA filtration.

I'm sure any of this would work so long as you give it the optimal parameters you can.


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(Avatar is Maxxy, a character by Mizzyam, RIP)


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InvisibleJacobStorm
psychedelic cartel
I'm a teapot


Registered: 07/21/15
Posts: 1,499
Re: First time grower and have a contamination concern. [Re: micro]
    #22303319 - 09/28/15 03:01 PM (8 years, 4 months ago)

Very interesting. Wish I had a spare bedroom for experiments. I'd either blow you all out of the water, or just blow my self up... Probably the second one lol.


--------------------
Rogger Rabbits Mushroom growing videos

Ethnobotanical garden forum

Inocuole tea TEK

azur said: If you like 6th grade results, then 6th grade projects are great.


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OfflineBL4Z3D247
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Re: First time grower and have a contamination concern. [Re: JacobStorm]
    #22308200 - 09/29/15 01:05 PM (8 years, 4 months ago)

So then I will need to fan to encourage evaporation when using a SGFC? This is why I'm asking because I have been reading so much and I'm not sure if everything I've read is up-to-date information or obsolete. Thanks for the responses everyone.


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