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OfflineMysticMoteToter
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Cloning from a dry mushroom?
    #22289180 - 09/25/15 04:31 PM (8 years, 4 months ago)

Hey everybody thanks for giving this a look. I hope this is the right forum to post this as i am by no means an advanced mycologist but i was just wondering if someone who is here would be willing to answer some questions about cloning a dry mushroom and the cloning process. Basically my questions are is
1) what is the best area to cut from a dry mushroom in order to effectively clone it on agar?
and
2) From my understanding when cloning a mushroom instead of getting spore prints and using spores to inoculate jars, instead you get mycelium growth on the agar plate. How can I, or is there a way to use this mycelium in the standard BRF PF Tek?
Thanks guys and i hope everyone has a great weekend!


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InvisibleToadstool5
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Re: Cloning from a dry mushroom? [Re: MysticMoteToter]
    #22289320 - 09/25/15 05:00 PM (8 years, 4 months ago)

Quote:

Dry tissue can in 'some' instances be revived, but it's certainly not something for a new grower to attempt without agar skills and a good laminar flowhood.

In a glovebox, you might have success germinating spores from the dried fruits on agar. The problem is being able to separate the mushroom mycelium from the natural contaminants that will also begin growing on your petri dishes.




Quote:

Try it with tissue a year or more old. Normally, it will take two to three weeks or more for mushroom mycelium to begin to grow from totally dry tissue. Often, growth starts sooner than that, but is almost always mold. If you have growth from completely dry tissue within a day and a half, it's almost a guarantee of mold. You can quickly determine that under a small microscope. Mushroom mycelium will be straight, while most molds will have an appearance of a steel wool pad with kinky, twisted mycelial strands with lots of side branching. Good luck.

Iodine is a great bactericide. I dip dry tissue first in iodine for a minute or two, then wash it off with a peroxide dip for five full minutes, then take the tissue through two distilled water washes before placing on the antibiotic agar. This procedure works for about a 10% success rate. Usually one or two of the cultures will grow mold, one or two will grow bacteria, one will grow mushroom mycelium, and the rest just sit there doing nothing. One of the keys for successful dry tissue recovery is to inoculate as many petri dishes as you can afford in order to use the numbers to your advantage.
RR




Quote:

If you still have problems with contaminants after using peroxide, you can also dip tissue into a ten percent bleach solution for a couple of minutes. Believe it or not, the mycelium can withstand this, but molds and bacteria can't. The bleach works really well when cloning dry tissue. Live tissue needs a week or two to recover from the bleach, but dry tissue is dormant so hardly even gets smacked.RR




Search for "clone dry tissue" in the advanced mycology threads and don't double post brah. :thumbup:

Good luck on the cloning!


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OfflineMysticMoteToter
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Re: Cloning from a dry mushroom? [Re: Toadstool5]
    #22289431 - 09/25/15 05:26 PM (8 years, 4 months ago)

thank you man sorry for not doing a quick search before. I think the bleach dip will be my best shot, thanks you man sorry again for not checking first i'm just getting antsy haha


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OfflineKizzle
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Re: Cloning from a dry mushroom? [Re: MysticMoteToter]
    #22304351 - 09/28/15 06:20 PM (8 years, 4 months ago)

It may not be possible depending on the mushroom was dried. Some people use dehydrators at temperatures that would make any spore or tissue nonviable.


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OfflineCj112321
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Re: Cloning from a dry mushroom? [Re: Kizzle]
    #22304656 - 09/28/15 07:17 PM (8 years, 4 months ago)

Does anyone please please know where a connect in DC VA or MD ...LSD :frown: #soberhippie


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OfflineKizzle
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Re: Cloning from a dry mushroom? [Re: Cj112321]
    #22304809 - 09/28/15 07:48 PM (8 years, 4 months ago)

:trollcop:


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OfflineMysticMoteToter
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Re: Cloning from a dry mushroom? [Re: Cj112321]
    #22310726 - 09/29/15 08:22 PM (8 years, 3 months ago)

Quote:

Cj112321 said:
Does anyone please please know where a connect in DC VA or MD ...LSD :frown: #soberhippie



No the whole country is dry.  :cop:


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