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Offlinevagblast
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Re: Agar Plates Have Growth!! When Do I Transfer? [Re: taGyo]
    #22335431 - 10/05/15 01:12 AM (8 years, 3 months ago)

:asianofapproval:


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InvisibleKalistis
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Re: Agar Plates Have Growth!! When Do I Transfer? [Re: vagblast]
    #22340359 - 10/06/15 09:53 AM (8 years, 3 months ago)

Day 4 after transfer 2- 3 plates have green contam. Otherwise, seemingly looking good. Give this is my 3rd plate- Do I let this one ride out a little more? MS>Wedge Transfer>Wedge Transfer



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Offlinevagblast
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Re: Agar Plates Have Growth!! When Do I Transfer? [Re: Kalistis]
    #22340402 - 10/06/15 10:10 AM (8 years, 3 months ago)

Id wait a couple more days to get some real good rhyzos and do another. Hopefully you will get urself a mono by then. If not no biggey do another transfer. Haha. Easy right? I love agar


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InvisibleKalistis
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Re: Agar Plates Have Growth!! When Do I Transfer? [Re: vagblast]
    #22340414 - 10/06/15 10:12 AM (8 years, 3 months ago)

I have monos-- multiple in fact.

Wait, are you telling me I can go from agar directly to monotub?? How the hell did I miss this? I thought I would have to go from agar to grain to monotub.

Tek?


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OfflineNDStepp84
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Re: Agar Plates Have Growth!! When Do I Transfer? [Re: Kalistis]
    #22340423 - 10/06/15 10:14 AM (8 years, 3 months ago)

He means a mono culture with no sectors on agar


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"I am free, no matter what rules surround me. If I find them tolerable, I tolerate them; if I find them too obnoxious, I break them. I am free because I know that I alone am morally responsible for everything I do.
-Robert A. Heinlein 

:takingnotes: Links and teks:takingnotes:
ND's grow log and discussion
Plant thread


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InvisibleKalistis
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Re: Agar Plates Have Growth!! When Do I Transfer? [Re: Kalistis]
    #22340424 - 10/06/15 10:14 AM (8 years, 3 months ago)

OMG what the hell am I going to do with all of these plates? I sure as hell am not going to spawn them all at once. Plus I already have 2 tubs already in colonization, and 12 jars of oats almost done colonizing. Hahaha. I think I may have bitten off way more than I can chew, let alone swallow.


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InvisibleKalistis
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Re: Agar Plates Have Growth!! When Do I Transfer? [Re: NDStepp84]
    #22340433 - 10/06/15 10:15 AM (8 years, 3 months ago)

Quote:

NDStepp84 said:
He means a mono culture with no sectors on agar




Oh. I don't expect a mono on 2nd transfer of MS.


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Offlinevagblast
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Re: Agar Plates Have Growth!! When Do I Transfer? [Re: Kalistis]
    #22340461 - 10/06/15 10:23 AM (8 years, 3 months ago)

Haha. Yeah i did the same. Endend up chunking alot of petris myself. Made some lc and put it in the frige. But yes normally if i do like 12 dishes with ms. Then by the first sometime or even the second ill have a mono. But by then ill have almost 35 dishes. But i wont have to transfer as many times and its alot faster for me to get enough monos to start knocking stuff up. Its a waste for sure but it takes half the time.


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InvisibleStickyIcky Fingers
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Re: Agar Plates Have Growth!! When Do I Transfer? [Re: Kalistis]
    #22340463 - 10/06/15 10:24 AM (8 years, 3 months ago)

If you have excess colonized clean plates you can stick them in the fridge to store and drop pieces to grain later. Clean out/ kill the dirty plates and you can add more agar later (I'm not sure if this works for disposable plastic plates, the PC may melt them)


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Offlinekushroom
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Re: Agar Plates Have Growth!! When Do I Transfer? [Re: vagblast]
    #22340472 - 10/06/15 10:26 AM (8 years, 3 months ago)

Quote:

vagblast said:
Its not.my idea exactly i just added a little to it. I used a soldering iron to melt.the edges of the discs.



But this is the original inventor.
http://www.shroomery.org/forums/showflat.php/Number/20115107

Check it out. I did it the same way just added my step and used only one layer for the top holes




Actually that seems to make even better since than ironing the whole material as that seems to prolly allow the pollyfil to breath still well, but at the same time maintaining consistent shape!


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:/



All submitted posts are by Someone Who Isn't Me (SWIM) -  and in any event are works of pure fiction or outright lies.  Any information, statement, or assertion contained therein should be considered pure unadulterated fictitious lies.


Edited by kushroom (10/06/15 10:27 AM)


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Offlinevagblast
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Re: Agar Plates Have Growth!! When Do I Transfer? [Re: vagblast]
    #22340485 - 10/06/15 10:29 AM (8 years, 3 months ago)



1 mono out of 12 dishes i transferred to from the first transfer from the ms. So second transfer. Lol if that makrs sence. Its labeled


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InvisibleKalistis
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Re: Agar Plates Have Growth!! When Do I Transfer? [Re: vagblast]
    #22340511 - 10/06/15 10:35 AM (8 years, 3 months ago)

I have a few promising plates.

Can I transfer whatever I want to save to a slant? I got tons of experience storing those.


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InvisibleKalistis
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Re: Agar Plates Have Growth!! When Do I Transfer? [Re: vagblast]
    #22340514 - 10/06/15 10:36 AM (8 years, 3 months ago)

Quote:

vagblast said:


1 mono out of 12 dishes i transferred to from the first transfer from the ms. So second transfer. Lol if that makrs sence. Its labeled




So pretty. You should frame that!


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InvisibleKalistis
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Re: Agar Plates Have Growth!! When Do I Transfer? [Re: Kalistis]
    #22340526 - 10/06/15 10:39 AM (8 years, 3 months ago)

I am really looking forward to doing my first print once these tubs fruit! I'm so proud of what I have accomplished so far that I want to take a snapshot of my fruit! Lol...


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InvisibleInocuole
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Re: Agar Plates Have Growth!! When Do I Transfer? [Re: Kalistis]
    #22340695 - 10/06/15 11:28 AM (8 years, 3 months ago)

Quote:

vagblast said:


1 mono out of 12 dishes i transferred to from the first transfer from the ms. So second transfer. Lol if that makrs sence. Its labeled





Quote:

megatacular said:
My first mono on the first transfer from ms syringe





Hey there's the first transfer! :lol:
It's like you didn't even try man.


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                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

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Offlinevagblast
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Re: Agar Plates Have Growth!! When Do I Transfer? [Re: Kalistis]
    #22340700 - 10/06/15 11:29 AM (8 years, 3 months ago)

Lol yeah shes a beut! And yea once u get a good mono culture do some slants. I wouldnt slant an unclean culture imo though. Plus once u decide to use a slant ur gonna wanna put a peace on agar again to confirm its still clean to be on the safe side. And to make enough to innoc. Better to add something clean too it first ya know? Save u some worry.


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OfflinecronicrFacebook
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Re: Agar Plates Have Growth!! When Do I Transfer? [Re: vagblast]
    #22340823 - 10/06/15 11:59 AM (8 years, 3 months ago)

i dont'even think he was smart enough to catch that inoc:lol:


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OfflineNDStepp84
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Re: Agar Plates Have Growth!! When Do I Transfer? [Re: cronicr]
    #22340834 - 10/06/15 12:01 PM (8 years, 3 months ago)

Thought the same thing :lmafo:


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"I am free, no matter what rules surround me. If I find them tolerable, I tolerate them; if I find them too obnoxious, I break them. I am free because I know that I alone am morally responsible for everything I do.
-Robert A. Heinlein 

:takingnotes: Links and teks:takingnotes:
ND's grow log and discussion
Plant thread


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InvisibleKalistis
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Re: Agar Plates Have Growth!! When Do I Transfer? [Re: NDStepp84]
    #22341168 - 10/06/15 01:55 PM (8 years, 3 months ago)

Perhaps they are the same person- two accounts? They are both from Texas. Or maybe that went over my head.


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InvisibleInocuole
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Re: Agar Plates Have Growth!! When Do I Transfer? [Re: Kalistis]
    #22341281 - 10/06/15 02:37 PM (8 years, 3 months ago)

http://www.shroomery.org/forums/showprofile.php?User=236968

I'm sure if you do a little digging between the two of them you'll figure it out.


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                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

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