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InvisibleKalistis
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Registered: 09/06/15
Posts: 2,265
Re: Agar Plates Have Growth!! When Do I Transfer? [Re: Kalistis]
    #22304597 - 09/28/15 07:02 PM (8 years, 4 months ago)

Oh wow that is beautiful! LOL! I pulled that crap so tight that I accidentally flipped one of the wedges. Maybe I need to cut less off the end of a tub or just use the Parafilm. Although the parafilm won't like being over saran wrap.


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InvisibleKalistis
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Registered: 09/06/15
Posts: 2,265
Re: Agar Plates Have Growth!! When Do I Transfer? [Re: Kalistis]
    #22316578 - 09/30/15 10:05 PM (8 years, 3 months ago)

All of my 1st wedge transfers look like the photos below. Out of the 9 remaining plates, 1 was contaminated. Time to transfer again?



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InvisibleFriedEggS
I'm a teapot


Registered: 09/22/14
Posts: 2,536
Loc: Taiwan
Re: Agar Plates Have Growth!! When Do I Transfer? [Re: Kalistis]
    #22316627 - 09/30/15 10:19 PM (8 years, 3 months ago)

yes, transfer again! 

plate 1:  transfer from 2:00
plate 2: transfer from.... uhhhh. maybe 11:00 or 1:00 :shrug:
plate 2: transfer from 10:00

you could also do multiple transfers per plate

good job :thumbup:


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(Yes, the egg is real)

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InvisibleKalistis
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Posts: 2,265
Re: Agar Plates Have Growth!! When Do I Transfer? [Re: FriedEgg]
    #22316634 - 09/30/15 10:22 PM (8 years, 3 months ago)

Yeah buddy! I have to make another batch of agar. Thanks!


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InvisibleKalistis
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Registered: 09/06/15
Posts: 2,265
Re: Agar Plates Have Growth!! When Do I Transfer? [Re: Kalistis]
    #22316716 - 09/30/15 10:47 PM (8 years, 3 months ago)



Love the new jars, BTW.... My only regret is that I didn't buy more.


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InvisibleFriedEggS
I'm a teapot


Registered: 09/22/14
Posts: 2,536
Loc: Taiwan
Re: Agar Plates Have Growth!! When Do I Transfer? [Re: Kalistis]
    #22316750 - 09/30/15 10:56 PM (8 years, 3 months ago)

yea those look nice :thumbup:


if you ever get tired of doing pours, look up pasty's no pour tek:
http://www.shroomery.org/forums/showflat.php/Number/19208976


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(Yes, the egg is real)

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OfflineDarkhome
•Shaman•N•Training•
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Registered: 07/10/15
Posts: 517
Loc: United States Flag
Last seen: 7 years, 1 month
Re: Agar Plates Have Growth!! When Do I Transfer? [Re: Kalistis]
    #22316788 - 09/30/15 11:09 PM (8 years, 3 months ago)

Quote:

Caelistis said:


Love the new jars, BTW.... My only regret is that I didn't buy more.



That is nice...thanx...lol I just bought one on Amazon when I saw yours.
Nice Job with your Agar BTW!


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“The shaman is not merely a sick man, or a madman; he is a sick man who has healed himself.”
~Terence McKenna~


"NOTHING"...I post or say is "TRUE"...Any pictures are taken from the Web and any conversation is just research for a fictional character. That being said; He is constantly seeking knowledge and evolving.


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InvisibleKalistis
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Posts: 2,265
Re: Agar Plates Have Growth!! When Do I Transfer? [Re: Darkhome]
    #22316806 - 09/30/15 11:20 PM (8 years, 3 months ago)

You should really buy 4. I bought two and as I am PC'sing my agar now I am completely regretting that I didn't buy 2 more. They are fricking awesome!! A little spendy, but totally worth it. I got the 500 ml bottles instead of the liter because I have small hands and wanted something small to handle in my SAB. I used a damn 750ml sake bottle last time and I spent more time worrying that I would drop the bottle that I overfilled some of my plates.

And thanks for the encouragement. This stuff is addicting! I am so close to spawning my first jars to bulk this weekend! I haven't even considered what I am going to do with all these mushrooms if I am successful.


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OfflineDarkhome
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Registered: 07/10/15
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Re: Agar Plates Have Growth!! When Do I Transfer? [Re: Kalistis]
    #22316816 - 09/30/15 11:25 PM (8 years, 3 months ago)

Thanx for the advice, I'll jump back on and order a few more, now that I know they work so well...
Having a ton of Mushies is a GREAT problem to have...lol
They keep pretty well if you dehydrate them to "cracker dry" with a food dehydrator... I highly recommend a dehydrator if you don't already have one! Keep up the GOOD WORK, You are Rockin' It:rockman:


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“The shaman is not merely a sick man, or a madman; he is a sick man who has healed himself.”
~Terence McKenna~


"NOTHING"...I post or say is "TRUE"...Any pictures are taken from the Web and any conversation is just research for a fictional character. That being said; He is constantly seeking knowledge and evolving.


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InvisibleKalistis
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Registered: 09/06/15
Posts: 2,265
Re: Agar Plates Have Growth!! When Do I Transfer? [Re: Darkhome]
    #22316830 - 09/30/15 11:30 PM (8 years, 3 months ago)

Hahaha I haven't even gotten there mentally, but at this rate with 24 quart jars and agar in the works with 2 syringes remaining, I am going to have more than I had ever imagined. It's somewhat terrifying how quickly this stuff accumulates and how I just keep trucking along. I guess I am starting to know what it feels like to be a hoarder or a crazy cat lady...


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InvisibleKalistis
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Re: Agar Plates Have Growth!! When Do I Transfer? [Re: Kalistis]
    #22316837 - 09/30/15 11:33 PM (8 years, 3 months ago)

And do I have a food dehydrator....? LOL!



I am a planner and don't do anything half ass. I also love Amazon. It feels like Christmas every evening when I get home from work and their is a package on my door step.


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OfflineDarkhome
•Shaman•N•Training•
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Registered: 07/10/15
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Re: Agar Plates Have Growth!! When Do I Transfer? [Re: Kalistis]
    #22316854 - 09/30/15 11:41 PM (8 years, 3 months ago)

Quote:

Caelistis said:
And do I have a food dehydrator....? LOL!



I am a planner and don't do anything half ass. I also love Amazon. It feels like Christmas every evening when I get home from work and their is a package on my door step.



Awesome...I am the exact same...Amazon makes me happy...maybe too happy...lol I added a rating to your profile... You gave me a good idea...and I am impressed with your Agar and Attitude!:rockon:


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“The shaman is not merely a sick man, or a madman; he is a sick man who has healed himself.”
~Terence McKenna~


"NOTHING"...I post or say is "TRUE"...Any pictures are taken from the Web and any conversation is just research for a fictional character. That being said; He is constantly seeking knowledge and evolving.


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Offlinemushpunx
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Re: Agar Plates Have Growth!! When Do I Transfer? [Re: Kalistis]
    #22320727 - 10/01/15 07:29 PM (8 years, 3 months ago)

Quote:

Caelistis said:
Oh wow that is beautiful! LOL! I pulled that crap so tightit.that I accidentally flipped one of the wedges. Maybe I need to cut less off the end of a tub or just use the Parafilm. Although the parafilm won't like being over saran wrap.




Yea it takes a little practice. I wish I could post a video cause there is kind of a trick to it

I think you're right, maybe cut the roll just a bit shorter and it'll make it easier to manage. That is the worst when you knock the wedge around and cant get it back in place, it leaves myc all over the place too when your platr grows out .
You wil LOVE parafilm though!


You are doing a nice job with your agar work, keep it up!!


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InvisibleKalistis
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Posts: 2,265
Re: Agar Plates Have Growth!! When Do I Transfer? [Re: mushpunx]
    #22325417 - 10/02/15 07:31 PM (8 years, 3 months ago)

Thanks, I am completing transfer #2 tonight! I'll post picture updates once I get some growth.


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InvisibleKalistis
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Posts: 2,265
Re: Agar Plates Have Growth!! When Do I Transfer? [Re: Kalistis]
    #22326114 - 10/02/15 10:04 PM (8 years, 3 months ago)

It feels good to be a gangsta. :grin:

Transferred a shit load of wedges to a plates poured 48 hours ago. The process keeps getting easier and easier as I go. This time around I tried two different recipes PDA and MEA... first two rounds were on MEA, but I felt like experimenting with different types of agar to see which recipe the mycelium liked more.

My plates are labeled and divided based on 3 things: Standard transfer, fuck up transfer (rolled around on agar or the petri dsh had more condensation than I liked), and contaminated transfer. Luckily I only had 2 contaminated plates from my last batch of 11. I didn't identify which were PDA or MEA because it is easy to identify by color alone.



So much fun.


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Offlinevagblast
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Registered: 10/04/10
Posts: 403
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Last seen: 8 years, 3 months
Re: Agar Plates Have Growth!! When Do I Transfer? [Re: Kalistis]
    #22330037 - 10/03/15 08:03 PM (8 years, 3 months ago)

I love agar. Its super fun!!!!! Great job og! Gotta try monos with my take on ironed polyfil discs for fae now a little different than dialateds tek. Lol im pround. I love exeriments!! Whooh!




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InvisibleKalistis
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Registered: 09/06/15
Posts: 2,265
Re: Agar Plates Have Growth!! When Do I Transfer? [Re: vagblast]
    #22330073 - 10/03/15 08:13 PM (8 years, 3 months ago)

Nice! I actually just came online right now to investigate building my monotub.

I like your idea. Did it work?


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Offlinevagblast
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Registered: 10/04/10
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Re: Agar Plates Have Growth!! When Do I Transfer? [Re: Kalistis]
    #22330381 - 10/03/15 09:30 PM (8 years, 3 months ago)

Its not.my idea exactly i just added a little to it. I used a soldering iron to melt.the edges of the discs.



But this is the original inventor.
http://www.shroomery.org/forums/showflat.php/Number/20115107

Check it out. I did it the same way just added my step and used only one layer for the top holes


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InvisibleKalistis
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Posts: 2,265
Re: Agar Plates Have Growth!! When Do I Transfer? [Re: vagblast]
    #22332251 - 10/04/15 11:20 AM (8 years, 3 months ago)

Here we are: Day 2 after transfer #2 and things are looking good. None of the plates are showing signs of contaminants. 3 of the PDA plates have condensation and the wedges flipped off to the side on 2 other plates, but I would say things are looking great!

Here's a sample of some of the plates:



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OfflinetaGyo
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Registered: 10/16/14
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Re: Agar Plates Have Growth!! When Do I Transfer? [Re: Kalistis]
    #22332298 - 10/04/15 11:34 AM (8 years, 3 months ago)

:popcorn:

They look good!


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