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Kalistis


Registered: 09/06/15
Posts: 2,265
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UPDATE!! Agar Plates Have Growth!! Finally made it to grains!
#22285552 - 09/24/15 06:55 PM (8 years, 4 months ago) |
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Many of my first attempt agar plates with Argentina MS have growth! Only two have no growth and 1 have a contaminant, but looks like I may still be able to isolate the mycelium out? What do you guys think? How long do I let the first round go? I was under the impression with MS you shouldn't let it go too long before transferring to a new plate.
Edited by Kalistis (10/13/15 03:05 PM)
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NDStepp84
Stellar nuclear waste


Registered: 04/23/15
Posts: 4,956
Last seen: 1 month, 3 days
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Re: Agar Plates Have Growth!! When Do I Transfer? [Re: Kalistis]
#22285589 - 09/24/15 07:01 PM (8 years, 4 months ago) |
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Sooner the better, nice nails.
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"I am free, no matter what rules surround me. If I find them tolerable, I tolerate them; if I find them too obnoxious, I break them. I am free because I know that I alone am morally responsible for everything I do. -Robert A. Heinlein
Links and teks ND's grow log and discussion Plant thread
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vagblast
shroomificator



Registered: 10/04/10
Posts: 403
Loc: the lone star
Last seen: 8 years, 3 months
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Re: Agar Plates Have Growth!! When Do I Transfer? [Re: NDStepp84]
#22285637 - 09/24/15 07:10 PM (8 years, 4 months ago) |
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I usually wait until I can divide a couple of good looking spots like u have there into three wedges and from m there make 6-12 new plates one or more will be pretty close to a mono then.with one.or.two more transfers I get my mono then just make how ever.many transfers I need from that. Just an idea.
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Kalistis


Registered: 09/06/15
Posts: 2,265
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Re: Agar Plates Have Growth!! When Do I Transfer? [Re: NDStepp84]
#22285847 - 09/24/15 07:59 PM (8 years, 4 months ago) |
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Quote:
NDStepp84 said: Sooner the better, nice nails.
Haha... Thanks. My nails and socks are my only forms of expression. I live in scrubs.
Do I throw these puppies in the fridge while my fresh plates cool?
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Kalistis


Registered: 09/06/15
Posts: 2,265
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Re: Agar Plates Have Growth!! When Do I Transfer? [Re: Kalistis]
#22285862 - 09/24/15 08:03 PM (8 years, 4 months ago) |
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Also, do I have to cut a wedge or could I use my inoculation loop to pick up a portion for transfer?
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NDStepp84
Stellar nuclear waste


Registered: 04/23/15
Posts: 4,956
Last seen: 1 month, 3 days
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Re: Agar Plates Have Growth!! When Do I Transfer? [Re: Kalistis]
#22285880 - 09/24/15 08:08 PM (8 years, 4 months ago) |
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Get a scalpel or an exacto knife, flame sterilize until red hot then cool it in the receiving dish. Cut out a small square, scoop it up and place it in the center of receiving plate. Wouldn't make much of a difference to put in the fridge if you are transferring soon
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"I am free, no matter what rules surround me. If I find them tolerable, I tolerate them; if I find them too obnoxious, I break them. I am free because I know that I alone am morally responsible for everything I do. -Robert A. Heinlein
Links and teks ND's grow log and discussion Plant thread
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Kalistis


Registered: 09/06/15
Posts: 2,265
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Re: Agar Plates Have Growth!! When Do I Transfer? [Re: NDStepp84]
#22285918 - 09/24/15 08:16 PM (8 years, 4 months ago) |
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it will probably be 2 days. A lot can happen in 2 days.
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NDStepp84
Stellar nuclear waste


Registered: 04/23/15
Posts: 4,956
Last seen: 1 month, 3 days
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Re: Agar Plates Have Growth!! When Do I Transfer? [Re: Kalistis]
#22285941 - 09/24/15 08:23 PM (8 years, 4 months ago) |
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True. Wouldn't hurt, may slow them down until you transfer.
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"I am free, no matter what rules surround me. If I find them tolerable, I tolerate them; if I find them too obnoxious, I break them. I am free because I know that I alone am morally responsible for everything I do. -Robert A. Heinlein
Links and teks ND's grow log and discussion Plant thread
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mushpunx
Fungus Punk



Registered: 04/20/14
Posts: 13,394
Last seen: 11 days, 10 hours
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Re: Agar Plates Have Growth!! When Do I Transfer? [Re: NDStepp84]
#22286014 - 09/24/15 08:42 PM (8 years, 4 months ago) |
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I agree , from the germination plate I take a transfer as soon as I can see clean, healthy growth. I take it when its grown out a little less than the size of a dime. Next plate I let grow out a little further so I can try to pick out aggressive growth. Third plate is usually good enough to put to grain
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 Amateur Mycologists United AMU Q&A
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NDStepp84
Stellar nuclear waste


Registered: 04/23/15
Posts: 4,956
Last seen: 1 month, 3 days
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Re: Agar Plates Have Growth!! When Do I Transfer? [Re: mushpunx]
#22286045 - 09/24/15 08:53 PM (8 years, 4 months ago) |
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Well said sir that's the way to go.
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"I am free, no matter what rules surround me. If I find them tolerable, I tolerate them; if I find them too obnoxious, I break them. I am free because I know that I alone am morally responsible for everything I do. -Robert A. Heinlein
Links and teks ND's grow log and discussion Plant thread
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Re: Agar Plates Have Growth!! When Do I Transfer? [Re: mushpunx]
#22286052 - 09/24/15 08:55 PM (8 years, 4 months ago) |
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Kalistis


Registered: 09/06/15
Posts: 2,265
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Re: Agar Plates Have Growth!! When Do I Transfer? [Re: Mad Season]
#22286107 - 09/24/15 09:08 PM (8 years, 4 months ago) |
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Thanks guys! I am going to throw these buggers into the fridge until tomorrow night... possibly Saturday night when I have time to do the transfer.
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Kalistis


Registered: 09/06/15
Posts: 2,265
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Re: Agar Plates Have Growth!! When Do I Transfer? [Re: Kalistis]
#22298598 - 09/27/15 01:24 PM (8 years, 4 months ago) |
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I'm about to transfer. Do I place wedge face up or face down on agar? I had a link once with pictures of the process, but I can't find it. Can someone help me out?
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Re: Agar Plates Have Growth!! When Do I Transfer? [Re: Kalistis]
#22298604 - 09/27/15 01:26 PM (8 years, 4 months ago) |
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It really doesn't matter . The entire wedge will get fluffy in 24-48 hours post transfer
Check out the improve your sterile techniques link in my signature if you wanna see how I do transfers
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Kalistis


Registered: 09/06/15
Posts: 2,265
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Re: Agar Plates Have Growth!! When Do I Transfer? [Re: Mad Season]
#22298999 - 09/27/15 03:02 PM (8 years, 4 months ago) |
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That wasn't nearly as hard as I thought it was going to be in my SAB. I will need to bring home some parafilm from work because working with saranwrap is ludicrous.
So of the 13 plates I had ready to go, 2 looked like they could possibly have contaminants brewing and 1 had too much agar so I threw them out. I transfer a total of 10 wedges from the 4 plates I had. One of the wedges flipped while I was wrapping the dish and rolled around on the agar before rolling back into position. I wonder how badly that plate is fucked up?
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Re: Agar Plates Have Growth!! When Do I Transfer? [Re: Kalistis]
#22299009 - 09/27/15 03:03 PM (8 years, 4 months ago) |
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If it was wrapped up, the rolly one should be fine
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Kalistis


Registered: 09/06/15
Posts: 2,265
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Re: Agar Plates Have Growth!! When Do I Transfer? [Re: Mad Season]
#22299177 - 09/27/15 03:42 PM (8 years, 4 months ago) |
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Yeah it was wrapped up! Yay! Thank you for your help.
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Kalistis


Registered: 09/06/15
Posts: 2,265
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Re: Agar Plates Have Growth!! When Do I Transfer? [Re: Kalistis]
#22304508 - 09/28/15 06:49 PM (8 years, 4 months ago) |
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Here are what my plates look like 24 hours after first transfer. They all look about the same.


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mushpunx
Fungus Punk



Registered: 04/20/14
Posts: 13,394
Last seen: 11 days, 10 hours
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Re: Agar Plates Have Growth!! When Do I Transfer? [Re: Kalistis]
#22304552 - 09/28/15 06:55 PM (8 years, 4 months ago) |
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ooks good man! Wrap them up better though.. cut a 1.5 " section off a roll of cling wrap if you don't have paradigm so you can wrap the edges and pull it tight
Ok maybe this is a bit sloppy too haha but it's the only pic I have around..
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 Amateur Mycologists United AMU Q&A
Edited by mushpunx (09/28/15 06:58 PM)
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Kalistis


Registered: 09/06/15
Posts: 2,265
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Re: Agar Plates Have Growth!! When Do I Transfer? [Re: mushpunx]
#22304580 - 09/28/15 07:00 PM (8 years, 4 months ago) |
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I brought some parafilm, but I figured I would use it after next transfer. I now it doesn't look like it, but this is saran wrap. The crap wouldn't stick to itself which seems ridiculous. But it was 2.5 inches cut off the end of a tube. It seems pretty tight, but I added tape at one end to hold it in place. Should I leave what is in place and add another layer?
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