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Invisiblemrbart4444
The mycelium whisperer


Registered: 09/13/14
Posts: 2,266
Should i start over from spore or transfer
    #22285198 - 09/24/15 06:05 PM (8 years, 4 months ago)

Okay so i have two plates one AA+ and one PE and i made them about 3 months ago and stored them in the refrigerator till today. Made some more plates today and should i start over from spore or transfer from what ive grown already. No visable signs of contam on the plates . The growth on the plates wasnt uniform though since there was some water in the plates and the spores got washed around but the plates are all white.


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Invisibleblackdust
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Registered: 02/28/09
Posts: 8,327
Re: Should i start over from spore or transfer [Re: mrbart4444]
    #22285281 - 09/24/15 06:19 PM (8 years, 4 months ago)

transfer?
whats the problem :lol:


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Invisiblestareatclouds
star eat clouds?
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Registered: 09/29/14
Posts: 9,887
Trusted Cultivator
Re: Should i start over from spore or transfer [Re: blackdust]
    #22285297 - 09/24/15 06:21 PM (8 years, 4 months ago)

Uh, absolutely make transfers from the stored plates. Why wouldn't you?


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InvisibleNumeroEno
I come from the land of lizards
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Registered: 07/24/14
Posts: 9,652
Loc: Gamehendge Flag
Re: Should i start over from spore or transfer [Re: blackdust]
    #22285305 - 09/24/15 06:23 PM (8 years, 4 months ago)

Plates can keep in the fridge for a while. Just use a different agar formula for the transfers. Put that myc to work!


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InvisibleFriedEggS
I'm a teapot


Registered: 09/22/14
Posts: 2,536
Loc: Taiwan
Re: Should i start over from spore or transfer [Re: NumeroEno]
    #22285321 - 09/24/15 06:27 PM (8 years, 4 months ago)

yup, transfer.  stored plates last quite a while in the fridge.


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Invisiblemrbart4444
The mycelium whisperer


Registered: 09/13/14
Posts: 2,266
Re: Should i start over from spore or transfer [Re: stareatclouds]
    #22285419 - 09/24/15 06:36 PM (8 years, 4 months ago)

Quote:

stareatclouds said:
Uh, absolutely make transfers from the stored plates. Why wouldn't you?



I was asking due to the possibility of contamination


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Invisibleblackdust
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Registered: 02/28/09
Posts: 8,327
Re: Should i start over from spore or transfer [Re: mrbart4444]
    #22285432 - 09/24/15 06:38 PM (8 years, 4 months ago)

:facepalm:
you are using agar

im just busting your balls.


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OfflineiSmkGrnBud
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Registered: 10/28/11
Posts: 891
Loc: Your subconscious
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Re: Should i start over from spore or transfer [Re: mrbart4444]
    #22285437 - 09/24/15 06:39 PM (8 years, 4 months ago)

You would be able to see any contamination just like a fresh plate. :thumbup:


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Offlinevagblast
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Registered: 10/04/10
Posts: 403
Loc: the lone star Flag
Last seen: 8 years, 3 months
Re: Should i start over from spore or transfer [Re: iSmkGrnBud]
    #22285660 - 09/24/15 07:14 PM (8 years, 4 months ago)

Are they monocultures?


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Invisiblestareatclouds
star eat clouds?
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Registered: 09/29/14
Posts: 9,887
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Re: Should i start over from spore or transfer [Re: mrbart4444]
    #22285722 - 09/24/15 07:33 PM (8 years, 4 months ago)

Quote:

mrbart4444 said:
I was asking due to the possibility of contamination




It's agar.


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Offlinemushpunx
Fungus Punk
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Registered: 04/20/14
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Re: Should i start over from spore or transfer [Re: stareatclouds]
    #22285853 - 09/24/15 08:00 PM (8 years, 4 months ago)

3 months? I would take a transfer just to be on the safe side. I know some folks  (myself included) who have had some problems using cultures direct out of cold storage

I'm pretty cautious though. Like after I pull from a slant I usually take one more transfer to be on the safer side :shrug:

But definitely no reason to start over from spore, even if you see some bacteria or something you can transfer away from it


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