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Frosty420
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Registered: 09/24/15
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Last seen: 8 years, 4 months
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New LC culture, possible contam would love feedback.
#22283821 - 09/24/15 01:00 PM (8 years, 4 months ago) |
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Here is my Honey and RO water PC 20 min 15 psi.
I have been reading and looking at pictures from what I read, having a "dusty" appearance can be a contam, but mine only looks dusty when i shake it up, otherwise it settles on the bottom and the rest of the jar is clear, which i read is good. Also the pictures I looked at some of the jars that were good looked like mine, and other were contam. I used a whole 10cc syringe packed with spores for this. The last pic is after i shook it up. Thanks in advance.



This is my first post, thanks for all the great info.
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: β
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Re: New LC culture, possible contam would love feedback. [Re: Frosty420]
#22284024 - 09/24/15 01:44 PM (8 years, 4 months ago) |
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You used a WHOLE SYRINGE of spores for an LC? I don't even have to look at it, that's definitely fucked. Why... did you do that? One drop would've been more than enough.
Who made the syringe? Are you sure it was 100% clean? Even one bacterium can ruin an LC. A 99.99% clean syringe has, on average, let's say, at LEAST a hundred contaminant spores, and every drop increases the chances of them germinating or otherwise reproducing. There's no way to know besides testing it but I wouldn't want to clean up the contamination it'll create.
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Frosty420
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Re: New LC culture, possible contam would love feedback. [Re: Inocuole]
#22284258 - 09/24/15 02:35 PM (8 years, 4 months ago) |
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The syringe is from online I don't really have a way of knowing if it is clean, since i purchased it.
the "reason" i used a whole syringe is because i have extra, and i figure that the jar would colonize faster growing all the spores rather than just a few & would decrease colonization time.
Then the picture is useless to look at and I should just start over?
Edited by Frosty420 (09/24/15 04:29 PM)
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: β
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Re: New LC culture, possible contam would love feedback. [Re: Frosty420]
#22284330 - 09/24/15 02:49 PM (8 years, 4 months ago) |
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http://www.shroomery.org/forums/showflat.php/Number/14960167
First read that, then edit your post so a mod doesn't come along and give you a warning.
More spores doesn't mean faster colonization time really, it just means a greater chance of failure. If it were some other type of inoculant more could be better but since spore syringes and prints are rarely 100% clean, it's always safest to use a little as possible. This is why it's important to use agar as an intermediary step if you plan to make large batches of inoculant, you want to be sure what you're using is clean and only agar can do that. I've grown pounds of mushrooms from a single drop of spore solution, so less is more, really.
http://www.shroomery.org/forums/showflat.php/Number/19208976
Also, having now looked at the pictures, it really doesn't look good there either.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: New LC culture, possible contam would love feedback. [Re: Inocuole]
#22284424 - 09/24/15 03:15 PM (8 years, 4 months ago) |
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if you make a LC from a spore syringe you should assume it's contaminated until it's proven clean.
also if you're going to go the stupid ass route of making a LC with spores use as little as possible like a drop or two. never a whole CC let alone 10cc.
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Frosty420
Stranger


Registered: 09/24/15
Posts: 6
Last seen: 8 years, 4 months
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Re: New LC culture, possible contam would love feedback. [Re: bodhisatta]
#22284699 - 09/24/15 04:24 PM (8 years, 4 months ago) |
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Do not we all assume the substrate is contaminated until otherwise proven? In fact that is the exact reason I uploaded pictures to get feedback on. Thanks for that, really really needed that clarification.
Edited by Frosty420 (09/24/15 04:31 PM)
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iSmkGrnBud
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Registered: 10/28/11
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Re: New LC culture, possible contam would love feedback. [Re: Frosty420]
#22284798 - 09/24/15 04:49 PM (8 years, 4 months ago) |
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Quote:
Frosty420 said: Do not we all assume the substrate is contaminated until otherwise proven? In fact that is the exact reason I uploaded pictures to get feedback on. Thanks for that, really really needed that clarification.
No because properly pasteurized substrate, or sterilized coir, is pretty damn contaminant resistant. We sometimes assume spawn is dirty when working with spawn from MS, but this why we work on agar so we can be sure our cultures are clean before we add them to grain for spawn. LC is hard to tell if there is a contaminant, and if there is, you're whole culture is fucked, and most of the times you cannot see the contaminant. With agar you can simply transfer away from a contaminant that is easily spotted with the eye.
-------------------- iSmkGrnBud's Teks iSmkGrnBud's Wild Mushroom Finds  The Noob Forum's List of Teks βThe probability of success is difficult to estimate; but if we never search the chance of success is zero.β -Giuseppe Cocconi
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: New LC culture, possible contam would love feedback. [Re: Frosty420]
#22285166 - 09/24/15 05:58 PM (8 years, 4 months ago) |
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Quote:
Frosty420 said: Do not we all assume the substrate is contaminated until otherwise proven? In fact that is the exact reason I uploaded pictures to get feedback on. Thanks for that, really really needed that clarification.
Hence test it...
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