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Toadstool5
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Registered: 01/22/15
Posts: 1,359
Loc: The Golden State
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I have seen some reports that wild type specimens are less likely to experience cell senescence so that is also a positive.
I wouldn't be surprised if any culture could be molded into a winning thoroughbred through breeding and isolation. All cultures were at some point a wild type and made into what they are today.
-------------------- If you do not know where the mushroom products you are consuming are grown, think twice before eating them. - Paul Stamets AMU Teks Stro's Write Ups
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drake89
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Registered: 06/26/11
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Quote:
poofterFroth said: There's actually lots of small & successful growers using many other shiitake cultures besides shiitake 3782.
Many of the field and forest shiiatke strains are very fruitful and don't cost much to aquire. 
they are shit on sawdust. most of their cultures are in fact, only notable one i can think of is PoHu. their cultures are mostly made for log growers. If you can't pull a pound or more on the first flush, you're wasting your time.
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confuzzed
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Re: Starting up shiitakes [Re: drake89]
#22324789 - 10/02/15 05:25 PM (8 years, 3 months ago) |
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Quote:
drake89 said:
Quote:
poofterFroth said: There's actually lots of small & successful growers using many other shiitake cultures besides shiitake 3782.
Many of the field and forest shiiatke strains are very fruitful and don't cost much to aquire. 
they are shit on sawdust. most of their cultures are in fact, only notable one i can think of is PoHu. their cultures are mostly made for log growers. If you can't pull a pound or more on the first flush, you're wasting your time.
Pound per 1st flush? Is that per bag, per straw log, per oak log?? Or is this pound per 1st flush to per certain weight of substrate?
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drake89
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Re: Starting up shiitakes [Re: confuzzed]
#22325094 - 10/02/15 06:30 PM (8 years, 3 months ago) |
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i'm talking about your standard 5-6lb sawdust bag. i average 1.25# of caps only (not counting stem weight) on the first flush.
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Sivarted
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Re: Starting up shiitakes [Re: drake89] 1
#22325223 - 10/02/15 06:58 PM (8 years, 3 months ago) |
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Turns out I may have spoken too soon. This morning when I left for work the shiitake plates all looked like they had given up the ghost, but right now 5 of 6 have exploded fuzzy white (the other has a small white patch), and my control plate (all I did was open it and move around in the SAB like I was pretending to cut a bit of mushroom for it) is clean, so hope survives.
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Sivarted
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Re: Starting up shiitakes [Re: Sivarted]
#22325242 - 10/02/15 07:03 PM (8 years, 3 months ago) |
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Of course, no guarantee that what I've got is actually going to end up as a viable culture, but what I'm seeing now is quite encouraging.
My plan from here is to let them go out a bit, do one or two transfers from each plate just to make sure they're clean, and then grow a bit of each one out into a cake (brf/sawdust/water) and see how they do.
Is it totally weird that I feel a little bit like a little kid with a brand new toy right now? Lol
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Sivarted
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Re: Starting up shiitakes [Re: Sivarted]
#22325268 - 10/02/15 07:08 PM (8 years, 3 months ago) |
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From there, my plan would be to take the best performers, spore print them, and start isolating from there, with the full knowledge that I'll probably be fruiting at least a couple dozen different isolates before I end up with something that looks good to take to a bag.
Unfortunately right now I can't dump the cash for the 3782, or I'd just do that, but in the meantime this is pretty exciting too, even if that's what I end up doing in the long run.
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Sivarted
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Re: Starting up shiitakes [Re: Sivarted] 1
#22325426 - 10/02/15 07:32 PM (8 years, 3 months ago) |
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Hard to see through the condensation in the photo, but definitely looks like I might have something here. 
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Toadstool5
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Registered: 01/22/15
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Re: Starting up shiitakes [Re: Sivarted]
#22326287 - 10/02/15 10:56 PM (8 years, 3 months ago) |
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Looks like it wants to grow 
Have you considered dying your agar with food coloring? I find a dark blue hue much easier to spot on than the agar color.
-------------------- If you do not know where the mushroom products you are consuming are grown, think twice before eating them. - Paul Stamets AMU Teks Stro's Write Ups
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Sivarted
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Re: Starting up shiitakes [Re: Toadstool5]
#22336713 - 10/05/15 12:14 PM (8 years, 3 months ago) |
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Two best dishes so far:

Prepped new pastyplates last night, going to do some transfers tonight.
Haven't opened them so it's hard to tell if there's any sectoring but the growth looks pretty even so I probably won't muck with isolating, under the assumption that the genetics are already reasonably consistent.
Edited by Sivarted (10/05/15 12:21 PM)
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Toadstool5
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Registered: 01/22/15
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Re: Starting up shiitakes [Re: Sivarted]
#22336938 - 10/05/15 01:26 PM (8 years, 3 months ago) |
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Thats the downside to pastyplates compared to petris the sectoring can be hard to see on growth that hasn't formed very distinct mycelial cords.
Either way it worked! 
Was the polyfil hard to fit into your lids? I have two layers of micropore tape on mine but I would feel much better with an actual filter. Maybe i'm just paranoid about something falling through the tape, I haven't had issues... Yet.
-------------------- If you do not know where the mushroom products you are consuming are grown, think twice before eating them. - Paul Stamets AMU Teks Stro's Write Ups
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Sivarted
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Re: Starting up shiitakes [Re: Toadstool5]
#22337218 - 10/05/15 02:54 PM (8 years, 3 months ago) |
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Really easy way to do polyfill on lids:
Drill a 1/4 inch hole (I've done this on both plastic and metal lids, sometimes trimming the edges of the hole is necessary especially if you try to go through a bunch at once)
Roll up a little ball of poly in your hands, hold it over the top of the hole, and stuff it in with a chopstick.
You can use the tip of the chopstick to pull polyfill from the edges of the ball and stuff it down into the hole, for varying amounts of tightness on the stuffing, and the underside ends up being a tidy little ball that doesn't require trimming or anything, and it only takes a few times to figure out how much poly to use for a good size and tightness.
So far I'm using it on pretty much everything, and you can even safely inject through polyfill from what I've read. Although I did make the mistake once of not letting the needle cool before trying to shove it through one time. Don't do that btw. Haha
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Toadstool5
A Registered Mycophile



Registered: 01/22/15
Posts: 1,359
Loc: The Golden State
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Re: Starting up shiitakes [Re: Sivarted]
#22337392 - 10/05/15 03:35 PM (8 years, 3 months ago) |
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Cool tip with the chopstick! I'll give it a shot.
Polyfil is my favorite for lids because its cheap, easy to find, and you can make cheap SFD's out of them with an iron. Thats my next goal, iron and silicon all my grain jar lids 
First I need to switch all my pastyplates over haha time to hunt down a chopstick.
-------------------- If you do not know where the mushroom products you are consuming are grown, think twice before eating them. - Paul Stamets AMU Teks Stro's Write Ups
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Sivarted
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Re: Starting up shiitakes [Re: Toadstool5]
#22337663 - 10/05/15 04:59 PM (8 years, 3 months ago) |
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Poly is really easy to work with after figuring out the chopstick thing (anything slender but not sharp enough to poke all the way through the polyfill would work just as well, I just happen to have lots of chopsticks), super cheap (the pillow I bought full of it cost me like $2 and will probably last a ridiculously long time), is very versatile, working as both a filter and an injection port, and is easy to replace if necessary, unlike anything you have to silicon on.
I just can't think of any good reason to use anything else, personally, at least not for jars and containers like these.
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Toadstool5
A Registered Mycophile



Registered: 01/22/15
Posts: 1,359
Loc: The Golden State
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Re: Starting up shiitakes [Re: Sivarted]
#22337699 - 10/05/15 05:13 PM (8 years, 3 months ago) |
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If I'm not mistaken tyvek and SFD are water-proof but polyfil stops filtering effectively if wet.
That's what I've been told, in my experience poly does still work if it only gets wet for a short time.
-------------------- If you do not know where the mushroom products you are consuming are grown, think twice before eating them. - Paul Stamets AMU Teks Stro's Write Ups
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Sivarted
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Re: Starting up shiitakes [Re: Toadstool5] 1
#22337770 - 10/05/15 05:32 PM (8 years, 3 months ago) |
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Not sure about tyvek, but sfds definitely need to not get wet as well.
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confuzzed
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Re: Starting up shiitakes [Re: Sivarted]
#22338091 - 10/05/15 06:52 PM (8 years, 3 months ago) |
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Quote:
drake89 said: i'm talking about your standard 5-6lb sawdust bag. i average 1.25# of caps only (not counting stem weight) on the first flush.
Cool! 
Quote:
Sivarted said: Not sure about tyvek, but sfds definitely need to not get wet as well.
Do you have a link for this? I am reading people PC in these in a way they get wet. I just bought these and I am also reading people bleach them, this would be "wet" too 
I will find out soon anyway, but I think they're supposed to be waterproof
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Sivarted
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Re: Starting up shiitakes [Re: confuzzed]
#22338404 - 10/05/15 08:11 PM (8 years, 3 months ago) |
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They can *get* wet (nothing that you ever PC is going to stay completely dry), and so can polyfill. They both just need to be dry again before you can expect them to be effective as a filter.
I would expect the same to be true of tyvek, but it seems to be more water resistant so I'm not sure.
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Sivarted
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Re: Starting up shiitakes [Re: Sivarted]
#22343431 - 10/06/15 11:08 PM (8 years, 3 months ago) |
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First set of transfers done!

Tough to get good photos of the plates I ended up transferring from, but here are the plates I *didn't* use:


The round ones are shiitake, squart ones are oysters. Two of those shiitake plates and all three oysters appear to have some sort of contamination, but I expected some with the cloning, and am pretty happy that I ended up with three clean plates of each with stronger growth than any of those show.
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Toadstool5
A Registered Mycophile



Registered: 01/22/15
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Loc: The Golden State
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Re: Starting up shiitakes [Re: Sivarted]
#22343489 - 10/06/15 11:29 PM (8 years, 3 months ago) |
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Yeah it happens with clones but luckily you were wise enough to do mutiple plates.
Congrats on the success! Time for further isolations or grains
-------------------- If you do not know where the mushroom products you are consuming are grown, think twice before eating them. - Paul Stamets AMU Teks Stro's Write Ups
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