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zalo_mb
Stranger

Registered: 09/15/15
Posts: 4
Last seen: 8 years, 4 months
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Help with possible contamination
#22281667 - 09/23/15 11:10 PM (8 years, 4 months ago) |
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Hi,
A few weeks ago I inoculated 5 jars. 3 of them are looking just fine, but the other 2 have something weird in the bottom.
This one looks fine to me, but I want to make sure it's ok. It looks like the mycelium reached one part from different ways, forming something like a ring.

The other one I don't know. It looks like verm that hasn't been colonized, but the color is a bit more grey. I read that something like this might happen if I used too much water. Should I discard it or just remove that part?

I hope you can help me out.
Thanks!
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ThePhoenix
Risen From Ashes


Registered: 04/20/14
Posts: 99
Last seen: 7 years, 6 months
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Re: Help with possible contamination [Re: zalo_mb]
#22281777 - 09/23/15 11:47 PM (8 years, 4 months ago) |
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If a jar is ever contained, always throw it out. Don't try to salvage it. Or you'll end up ruining everything else too. Your jars look like they have too much water like you said
-------------------- PM me for print trades! Looking for Cambodians, Mazatapec, or Penis Envy I have Golden Teacher Prints
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Re: Help with possible contamination [Re: ThePhoenix]
#22281938 - 09/24/15 01:13 AM (8 years, 4 months ago) |
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I must disagree and agree with you . Most contaminants get tossed. But both those jars are showing bacteria(bacillus/wet spot). It is common for myc to colonize it and "contain" it, but much like the criminals in jail, it is very much alive. This will cause a lower yield and a higher chance for contaminations like trich. Thus it really is up to the user if he wants to fruit it or not. If op has a limited # of jars, I'd say he can go ahead and try fruiting it. I've had plenty success from bacterial shit.
Also it's always been my experience that contaminations in your fruiting room, or from fruiting won't effect future grows. What really matters for true success is agar work/culture clean up, and having perfect techniques in your still air box. Almost all contamination comes from either a syringe or bad inoculation techniques, assuming you have done proper sterilization and have filtered gas exchange during colonization.
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micro
bunbun has a gungun



Registered: 05/09/03
Posts: 7,532
Loc: Brick City
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Re: Help with possible contamination [Re: Mad Season]
#22282544 - 09/24/15 08:19 AM (8 years, 4 months ago) |
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Quote:
Mad Season said: Also it's always been my experience that contaminations in your fruiting room, or from fruiting won't effect future grows..
Unless it's a pathogen...
Bacteria like that though, you might get away with it.
I'd still toss it personally because I wouldn't want to grow shrooms off bacteria, anyway :V
Also, pic 1 looks like the mycelium already overtook it.
If you *are* going to fruit it I'd cut that part out or it's just a vector for contamination.
-------------------- Any research paper or book for free (Avatar is Maxxy, a character by Mizzyam, RIP)
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zalo_mb
Stranger

Registered: 09/15/15
Posts: 4
Last seen: 8 years, 4 months
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Re: Help with possible contamination [Re: micro]
#22282655 - 09/24/15 08:51 AM (8 years, 4 months ago) |
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Thank you for your help!
And in comparison between the two, is the first one "better" in some sense? Or are they both equally risky when trying to fruit them?
And if I try to fruit them, is there a way of knowing if I cut the bacteria off successfully? Will the mushroom show something weird?
Thanks again guys! This is really helpful
Edited by zalo_mb (09/24/15 09:06 AM)
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TheEaglesGift
The Nagual


Registered: 04/10/11
Posts: 10,554
Loc: Ixtlan, Mexico
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Re: Help with possible contamination [Re: Mad Season]
#22284441 - 09/24/15 03:19 PM (8 years, 4 months ago) |
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Quote:
Also it's always been my experience that contaminations in your fruiting room, or from fruiting won't effect future grows.
You know I like you Mad, but I have to disagree with this statement.
Quote:
If you *are* going to fruit it I'd cut that part out or it's just a vector for contamination.
It doesn't have to be cut off. Any uncolonized portions can be easily washed off under the faucet before dunking.
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Re: Help with possible contamination [Re: TheEaglesGift]
#22284871 - 09/24/15 05:01 PM (8 years, 4 months ago) |
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Well I know for a fact that all of us have seen our fair share of trich. I know my room is laden with spores beyond belief. When I get trich, I've always blamed spawn or agar (since my substrate materials can be left out unspawned and hydrated for months with no contams.. ime it's the grains that contaminate or will cause a contamination), and strived to achieve clean agar and spawn. I've never bleached my room, I don't own a hood or anything to scrub the air, and I rarely sanitize tubs. Things are still going fine for me, but.. I also live in a dry ass environment. 
So if it's almost impossible to get a substrate to contaminate(at least that's my experience), wouldn't spawn/agar work be what truly matters? In that case all your SAB work, prep work and your filters are what really matters to achieve success. Thus it's my belief that what matters is proper sterilization/pasteurization, and sterile procedures/clean spawn. Anything in fruiting shouldn't have contaminated because of the fruiting room, it's effected because of the shitty spawn. I see further evidence of this when aggressive, clean cultures succeed. And when weak/bacterial cultures contaminate because they weren't able to fight it off. But correct me if I'm wrong. This is just my experience/opinions here.
I hope you at least understand my jibber jabber
Edited by Mad Season (09/24/15 05:21 PM)
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micro
bunbun has a gungun



Registered: 05/09/03
Posts: 7,532
Loc: Brick City
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Re: Help with possible contamination [Re: Mad Season]
#22285048 - 09/24/15 05:35 PM (8 years, 4 months ago) |
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Quote:
Mad Season said: When I get trich, I've always blamed spawn or agar (since my substrate materials can be left out unspawned and hydrated for months with no contams..
what is your substrate made out of?
peat moss? :v
-------------------- Any research paper or book for free (Avatar is Maxxy, a character by Mizzyam, RIP)
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Re: Help with possible contamination [Re: micro]
#22285556 - 09/24/15 06:56 PM (8 years, 4 months ago) |
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I've been doing mostly just hpoo. Some straw experiments too, but I haven't left hydrated straw alone before.
Like I said, it could just be that I'm in a dry environment I know most molds don't thrive too well up here in plant hardiness zone 2. It makes sense tho since I rarely see manure covered in molds outdoors, even if it's flooded and extremely wet.
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TheEaglesGift
The Nagual


Registered: 04/10/11
Posts: 10,554
Loc: Ixtlan, Mexico
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Re: Help with possible contamination [Re: Mad Season]
#22286919 - 09/25/15 02:16 AM (8 years, 4 months ago) |
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Mad, you wouldn't mind if I walked into the center of your fruiting room and smashed 10 trichoderma colonized quart jars onto the floor?
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Re: Help with possible contamination [Re: TheEaglesGift]
#22287405 - 09/25/15 07:53 AM (8 years, 4 months ago) |
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Definitely mind . But im not convinced it'd make me not be able to grow anymore. But I've also never done that before .
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TheEaglesGift
The Nagual


Registered: 04/10/11
Posts: 10,554
Loc: Ixtlan, Mexico
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Re: Help with possible contamination [Re: Mad Season]
#22288946 - 09/25/15 03:34 PM (8 years, 4 months ago) |
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I know some growers on these boards who've had to basically move because of vert in their homes, and went so far as to toss all their cultures just to make sure they brought as little with them as possible. Keeping the spore load in our homes is a paramount priority, or should be... that said, if we can obtain clean cultures, which isn't rocket science, then one can grow successfully in some relatively filthy quarters.
Still- I just personally wouldn't go so far as to say that contamination in your fruiting room won't effect future grows. It's just such an odd statement to me.
We'll agree to disagree Mad.
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