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newrook
Sucks at bulk



Registered: 03/20/15
Posts: 657
Last seen: 3 months, 4 days
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Quote:
noobie718 said:

Why'd you flip your jars?
And the dry verm layer on top is your contam barrier, leave em alone beyond that - no tinfoil, no ziptied coffee filters, no nothing. Leave them alone, the more you are handling the more you are fucking them up.
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  THROW AWAY YOUR SGFC
Edited by newrook (09/23/15 07:41 PM)
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mushpunx
Fungus Punk



Registered: 04/20/14
Posts: 13,394
Last seen: 11 days, 9 hours
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Huh? Did you not use a dry verm layer? Disturbing it can cause dirt to fall into your BRF. Unless u skipped it and used a grain lid filter
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 Amateur Mycologists United AMU Q&A
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: my firsrt grow [Re: mushpunx]
#22282615 - 09/24/15 08:37 AM (8 years, 4 months ago) |
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Coffee filters literally don't do shit compared to the dry verm barrier, if you did in fact make one. Mold spores can drive an envoy of semi trucks through a coffee filter. They could lead Mold Air Force One right in there. They don't have to sneak in one by one, they can fit the whole Moldy Trojan Horse inside.
If you did make one, it's extremely endangered by being upside down. And your logic is extremely endangered by allowing yourself to use stuff like cofffee filters without any concern for whether they actually work. These endangered species cannot be allowed to perish. Like, save the whales, man.
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noobie718
Stranger

Registered: 09/14/15
Posts: 28
Last seen: 8 years, 3 months
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Re: my firsrt grow [Re: Inocuole]
#22282705 - 09/24/15 09:03 AM (8 years, 4 months ago) |
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Yeah I did a dry verm layer, the tek I read said to use the filters I was just following what I read, granted that was before I discovered the shroomery. So even handling the jars can fuck em up ? just to check I'm cool without micro pore tape or anything as long as I have the dry layer? But since I already have the filters on there would it be best to just leave them alone now ? I'm probably gonna do that. And I'm debating not birthing until they just start to pin, and is it ok to add cakes to the fc as they become ready to fruit or is that a no no ? Sorry about all of the questions hopefully I'll be able to contribute later on
Edited by noobie718 (09/24/15 09:13 AM)
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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The very last question you asked is okay, and you can let them pin first if you really want. I wouldn't suggest doing anything else. I don't even know if I would turn them back upright. The damage could already be done.
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noobie718
Stranger

Registered: 09/14/15
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Last seen: 8 years, 3 months
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Re: my firsrt grow [Re: Inocuole]
#22282833 - 09/24/15 09:32 AM (8 years, 4 months ago) |
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Is it gravity that effects the myc or possibly contaminating the jars from turning them up side down?. I turned them upright last night and when I woke up this morning the jar to the far left all the myc has connected all the way around the jar and all other jars already show more progress just after 10 hours, I hope I didn't blindly fuck up my first grow.
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noobie718
Stranger

Registered: 09/14/15
Posts: 28
Last seen: 8 years, 3 months
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ill keep progress pics posted also for any one who is interested or has any comments, criticism or advice, I'm an information junkie (not only information). I'm not going to use my burma ms syringe until these gt's are complete (I don't have the space right now)
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Ajahn Don
Stranger


Registered: 07/05/15
Posts: 482
Loc: The buckle of the Bible belt
Last seen: 9 months, 1 day
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Re: my firsrt grow [Re: Dionili]
#22283096 - 09/24/15 10:24 AM (8 years, 4 months ago) |
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Quote:
Dionili said: so is 90% iso alcohol and bleach. 100% iso is better but you pretty much gotta order it.
This is at odds with what a number of people say. They say 70% is better than 90% iso for sanitizing.
Noobie, you won't be for long. I recommend working with PF-tek, brf cakes and an SGFC until you get really comfortable with growing mushrooms. Almost foolproof and will get you into a lot of the issues of growing, fast. I have found bulk and grain a lot more difficult. More variables. I suspect you'll be having a field day with agar soon, too.
Welcome to this incredily deep hobby. Mad scientists are encouraged here.
-------------------- "He's not altogether dense, but he's not altogether there."
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Quote:
Ajahn Don said:
Quote:
Dionili said: so is 90% iso alcohol and bleach. 100% iso is better but you pretty much gotta order it.
This is at odds with what a number of people say. They say 70% is better than 90% iso for sanitizing.
Yes it very much is. Anything much greater than 70% lacks the pronounced ability to penetrate cell walls. Too anhydrous at that point. Might as well be using 20% for as effective as 90% or greater is.
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Dionili
Second Rate Mycologist



Registered: 08/18/09
Posts: 2,194
Loc: Between a Rock and a Hard...
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Re: my firsrt grow [Re: Inocuole]
#22283604 - 09/24/15 12:20 PM (8 years, 4 months ago) |
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Quote:
Inocuole said:
Quote:
Ajahn Don said:
Quote:
Dionili said: so is 90% iso alcohol and bleach. 100% iso is better but you pretty much gotta order it.
This is at odds with what a number of people say. They say 70% is better than 90% iso for sanitizing.
Yes it very much is. Anything much greater than 70% lacks the pronounced ability to penetrate cell walls. Too anhydrous at that point. Might as well be using 20% for as effective as 90% or greater is.
Quote:
Inocuole said:
Quote:
Ajahn Don said:
Quote:
Dionili said: so is 90% iso alcohol and bleach. 100% iso is better but you pretty much gotta order it.
This is at odds with what a number of people say. They say 70% is better than 90% iso for sanitizing.
Yes it very much is. Anything much greater than 70% lacks the pronounced ability to penetrate cell walls. Too anhydrous at that point. Might as well be using 20% for as effective as 90% or greater is.
True, The reason alcohol disinfects is because it denatures bacteria This is also the reason is works on a wide selection of organisms. the stronger the concentration the quicker the (denaturalization? ) occurs. when this happens to quickly the cells become harder to penetrate.
So i was wrong. I just assumed since there was a quicker denaturization that it would infact disinfect better.
Learn somthin' new every day
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: my firsrt grow [Re: Dionili]
#22283620 - 09/24/15 12:22 PM (8 years, 4 months ago) |
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90% is good for... cleaning your bong.
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,887
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Re: my firsrt grow [Re: Dionili]
#22283741 - 09/24/15 12:45 PM (8 years, 4 months ago) |
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Quote:
Dionili said: Untrue. I've never had an issue. Just because you can isolate a mono culture and just because it grows faster/better/what ever does not mean MS innoc will always contam. If done right and with properly made syringes you will not have a problem with MS injections
Only if your sterile procedure sucks will you have issues with contamination.
This is really bad advice. Being results oriented with spores > LC is pretty silly. Inocuole isn't saying multi-spore is bad. He's saying multi-spore from a spore syringe to LC is bad because you have no way of knowing if it's clean or not. Because you don't.
Properly made syringes is the key here and syringes often contain dirty spores.
Edited by stareatclouds (09/24/15 12:53 PM)
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,887
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Quote:
noobie718 said: Is it gravity that effects the myc or possibly contaminating the jars from turning them up side down?. I turned them upright last night and when I woke up this morning the jar to the far left all the myc has connected all the way around the jar and all other jars already show more progress just after 10 hours, I hope I didn't blindly fuck up my first grow.
It's you disturbing the dry verm barrier which is protecting your substrate from the falling contaminants. The more you fuck with it, the more you displace it, and the more likely it is that contaminants will fall through. You're probably cutting off the gas exchange as well, which can force your jars to stall out due to high CO2 levels.
Just leave the jars right side up and don't touch them at all.
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Syringes contain dirty what?
Nah, stare's got it down.
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Ajahn Don
Stranger


Registered: 07/05/15
Posts: 482
Loc: The buckle of the Bible belt
Last seen: 9 months, 1 day
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Re: my firsrt grow [Re: Inocuole]
#22286122 - 09/24/15 09:11 PM (8 years, 4 months ago) |
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Quote:
Inocuole said: Syringes contain dirty what?
Nah, stare's got it down. 
Indeed, stare's the man.
-------------------- "He's not altogether dense, but he's not altogether there."
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noobie718
Stranger

Registered: 09/14/15
Posts: 28
Last seen: 8 years, 3 months
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Here's an update
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Ajahn Don
Stranger


Registered: 07/05/15
Posts: 482
Loc: The buckle of the Bible belt
Last seen: 9 months, 1 day
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Quote:
noobie718 said: Here's an update
Nothing happened.
-------------------- "He's not altogether dense, but he's not altogether there."
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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noobie718
Stranger

Registered: 09/14/15
Posts: 28
Last seen: 8 years, 3 months
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Re: my firsrt grow [Re: Inocuole]
#22385472 - 10/15/15 08:33 PM (8 years, 3 months ago) |
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Here's an new update notice the discoloration on the top of the cake on the right I'm worried it is contam but unsure if it could be bruised any input, this is five days after birth
Edited by noobie718 (10/15/15 08:34 PM)
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Kalistis


Registered: 09/06/15
Posts: 2,265
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Looks like bruising to me, but I haven't done cakes just bulk tubs. How's the FAE?
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