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Offlinenoobie718
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my firsrt grow
    #22278305 - 09/23/15 11:10 AM (8 years, 4 months ago)

I had experienced cubes on and off due to availability quality and the typical, I decided to start researching the subject a few months ago and decided that I would like to some microscopic study's to further my knowledge and being a first timer, a complete and utter noob I decided to explore the educational value of the pf tek (brf and verm) as a starting point, before I found this forum I was I could say now blindly following one of many pf teks I had found on the web and of course took that teks advice as to where to procure viable spores for my research, so I ordered from a vendor of which I found out later on this forum was essentially providing products that were not viable, so I followed the tek exactly (I am blessed to have equipment acquired from the local college chemistry and biological studies lab after renovations, another mans trash is another ones treasure) proper sterilization of substrates and surrounding environment and ratios I believe to be followed to a T , I am at 7 days on 1 brf/verm jars with no sign of germination or contamination  ( not completely worried yet) at a stable temp of 78*F I processed my own brf, I decided to do an LC in attempt to check for viability same temp I used honey at 4% LC is at day 3 and has whit film on the surface of the water which clings to the side when jar is tilted (very interesting) and white specs float around, I'm assuming germination has occurred but still to early to tell. I am at work and have to stop here I will post up dates with pictures any criticism is welcome.

his is day 3 of  LC. 9-12-15,  there was a white whispy film on the surface of the water and some specs but mostly clear. Still no activity in brf jar same syringe ( golden teachers)



9-13-15 the film on the top of the LC was was gone and it looked like this. turned almost clear. 







9-23-15 this is my brf jar so far,



9-23-15 i now have 4 more brf jars and i leaned up on the water in my ratio, which i suspected was the reason my first jar took so long to show any signs of growth/germinate because the 4 new jars only took 6 days on the same spore syringe and are taking off like damn it. will post pics tonight.



heres an update 10-6-15 100% colonized, been consolidating for 3 days
going to dunk and roll Friday, I have doubts about the jar that you can see the verm I cant tell if its cob web or if dry layer was to deep on that one and the myc got thin and wispy when it got to the dry layer.. any opinions ???????




Edited by noobie718 (10/06/15 06:28 PM)


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OfflineDionili
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Re: my firsrt grow [Re: noobie718]
    #22278481 - 09/23/15 12:07 PM (8 years, 4 months ago)

lookin good buddy


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Offlinenoobie718
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Re: my firsrt grow [Re: Dionili]
    #22278513 - 09/23/15 12:14 PM (8 years, 4 months ago)

Thank you very much, lots of priceless knowledge here in the shroomery


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InvisibleInocuole
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Re: my firsrt grow [Re: noobie718]
    #22278560 - 09/23/15 12:27 PM (8 years, 4 months ago)

Inoculating LC with spores is bad news.  You'll have to count on having really good luck for that not to go south.


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OfflineDionili
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Re: my firsrt grow [Re: Inocuole]
    #22278614 - 09/23/15 12:39 PM (8 years, 4 months ago)

Quote:

Inocuole said:
Inoculating LC with spores is bad news.  You'll have to count on having really good luck for that not to go south.





Untrue. I've never had an issue. Just because you can isolate a mono culture and just because it grows faster/better/what ever does not mean MS innoc will always contam. If done right and with properly made syringes you will not have a problem with MS injections


Only if your sterile procedure sucks will you have issues with contamination.

pressure canner is your best friend. so is 90% iso alcohol and bleach. 100% iso is better but you pretty much gotta order it.


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Offlinenoobie718
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Re: my firsrt grow [Re: Inocuole]
    #22278643 - 09/23/15 12:44 PM (8 years, 4 months ago)

i read a thread that a guy wrote about extracting a piece from the center of a stem with a hypo and then pushing it out into the LC solution that struck my interest. what would you suggest for LC? or is it even worth my time as a noob? or should i abandon LC all together and lean more toward making my own prints? that LC was more to determine if my spores were viable, guess i could have used agar, i also made a stupid mistake and didn't make a proper LC jar and when i stuck my hypo into the jar it was under vacuum and sucked a shit load of spore solution into the jar.


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OfflineDionili
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Re: my firsrt grow [Re: noobie718]
    #22278683 - 09/23/15 12:52 PM (8 years, 4 months ago)

Quote:

noobie718 said:
i read a thread that a guy wrote about extracting a piece from the center of a stem with a hypo and then pushing it out into the LC solution that struck my interest. what would you suggest for LC? or is it even worth my time as a noob? or should i abandon LC all together and lean more toward making my own prints? that LC was more to determine if my spores were viable, guess i could have used agar, i also made a stupid mistake and didn't make a proper LC jar and when i stuck my hypo into the jar it was under vacuum and sucked a shit load of spore solution into the jar.





lol newbie mistake. happens. well, was the jar at least cool? i doubt  you will have an issue. i make hone and LME Lc's and innoc with MS all the time and have no issues. as long as you make a test jar first for common sense rather ruin one then a whole batch. as for that hypo tek i'm not familiar with it. i have heard of others using a scalpel to remove chunks and clone via LC but i do not recommend that. if you need to clone i suggest cloning to agar and isolating a mono culture. its not hard. just requires some sterile procedure. you can use pastywhites agar tek or pour your own agar petris, or order some pre poured petries (sterile and sealed) and save some time and eliminate a contamination variable.

How ever since you are new my advice is to make plenty of grain jars, get a few more syringes, a few more lc jars and make as many as possible while practicing different types of sterile procedure until you figure it out. dont worry about cloning and agar until you are able to grow with out an issue.


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InvisibleInocuole
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Re: my firsrt grow [Re: Dionili]
    #22278795 - 09/23/15 01:12 PM (8 years, 4 months ago)

The more spore solution you injected, the more likely it is that it will have failed.


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Offlinenoobie718
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Re: my firsrt grow [Re: Dionili]
    #22279001 - 09/23/15 01:59 PM (8 years, 4 months ago)

Thanks for the advice, it is rather hard being a first timer because there are so many routes and paths to take, and choices when it comes to teks and what not. it is a bit overwhelming, but as many have said "read the forums" i agree with what you said, ill master the basics and go from there because what Ive come to under stand from reading the forums is that most all seasoned growers started with simple teks and eventually developed their own style.. so it probably would be wise to test my LC with agar ? my order just arrived from night train ! i got burmas. what is your opinion of grain vs brf ?

( i did let the jars cool over night before :syringe:)


Edited by noobie718 (09/23/15 02:04 PM)


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OfflineDionili
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Re: my firsrt grow [Re: noobie718]
    #22279010 - 09/23/15 02:03 PM (8 years, 4 months ago)

I started with bulk spawn. Did a couple of pf teks too


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InvisibleMad Season
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Re: my firsrt grow [Re: Dionili]
    #22279039 - 09/23/15 02:11 PM (8 years, 4 months ago)

Quote:

Mad Season said:
You had fine success. This is from a syringe i had the luck of getting.



This particular syringe in lc would have been shit. Luckily I used agar, and was able to salvage it. As seen by me cutting it.

Agar is just as easy to make as LC, and it's much safer. If it's clean spores, great! :thumbup: but I like getting success every time.



That's what I have to say about LC's. I'd say about 50% of the syringes I've gotten had some sort of contamination in them. Maybe I'm just weird, but I totally advocate agar to noobs. It's no harder to do than jello or LC's, and is the best way to improve sterile procedures in your still air box.


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InvisibleInocuole
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Re: my firsrt grow [Re: Mad Season]
    #22279610 - 09/23/15 04:37 PM (8 years, 4 months ago)

Quote:

Mad Season said:
Quote:

Mad Season said:
You had fine success. This is from a syringe i had the luck of getting.



This particular syringe in lc would have been shit. Luckily I used agar, and was able to salvage it. As seen by me cutting it.

Agar is just as easy to make as LC, and it's much safer. If it's clean spores, great! :thumbup: but I like getting success every time.



That's what I have to say about LC's. I'd say about 50% of the syringes I've gotten had some sort of contamination in them. Maybe I'm just weird, but I totally advocate agar to noobs. It's no harder to do than jello or LC's, and is the best way to improve sterile procedures in your still air box.



:whathesaid:


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Re: my firsrt grow [Re: Inocuole]
    #22280081 - 09/23/15 06:35 PM (8 years, 4 months ago)

Agreed with inoc, i don't care what anybody says about there success rate with spores to lc i will tell ya strait up it's not the best route to take, if the syringes are clean you can make well over 20 from a print anyway and have 200 jars colonzed in the same timeit would take to colonize your lc from the start....agar man FTW


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Offlinenoobie718
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Re: my firsrt grow [Re: Mad Season]
    #22280246 - 09/23/15 07:16 PM (8 years, 4 months ago)

wow what a pic, guess I had beginners luck considering I used ms


Edited by noobie718 (09/23/15 07:20 PM)


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Offlinenoobie718
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Re: my firsrt grow [Re: noobie718]
    #22280272 - 09/23/15 07:21 PM (8 years, 4 months ago)

any ideas on a quickie fc I was thinking shot gun style I hear a lot about those? so basically agar will allow me to check viability of ms recognize and isolate contam all in one shot sounds like a winner to me


Edited by noobie718 (09/23/15 07:25 PM)


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Re: my firsrt grow [Re: noobie718]
    #22280290 - 09/23/15 07:24 PM (8 years, 4 months ago)

yep sgfc is the go to! mono's for bulk....gh's kick ass too but i would keep it simple for now


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Offlinemushpunx
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Re: my firsrt grow [Re: noobie718]
    #22280307 - 09/23/15 07:31 PM (8 years, 4 months ago)

Yea man it seems most of us have beginners luck

I went ages just putting spore solution to grain before I ever saw a green tub. I thought I was just *that* good :lol:


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Offlinenoobie718
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Re: my firsrt grow [Re: mushpunx]
    #22280315 - 09/23/15 07:32 PM (8 years, 4 months ago)



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InvisibleInocuole
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Re: my firsrt grow [Re: mushpunx]
    #22280317 - 09/23/15 07:33 PM (8 years, 4 months ago)

Quote:

mushpunx said:
Yea man it seems most of us have beginners luck

I went ages just putting spore solution to grain before I ever saw a green tub. I thought I was just *that* good :lol:




Same here, bad times.



Why are those PF jars upside down??


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Offlinenoobie718
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Re: my firsrt grow [Re: noobie718]
    #22280333 - 09/23/15 07:38 PM (8 years, 4 months ago)



I fucked my hand up and wanted to put coffee filters on (over kill) so I flipped me on the filter held jar with arm and zip tied with good hand.. Yeah I'm a little ocd lol


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