Home | Community | Message Board

Magic-Mushrooms-Shop.com
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Mushroom-Hut Liquid Cultures   Original Sensible Seeds Bulk Cannabis Seeds   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Left Coast Kratom Buy Kratom Extract   North Spore Injection Grain Bag

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
OfflineMiNdTwIsTeR
ThirdEye

Registered: 10/14/08
Posts: 427
Last seen: 7 years, 3 hours
Multiplying spore syringes?
    #22277282 - 09/23/15 01:40 AM (8 years, 4 months ago)

I've read over and over that there's an over abundance of spores in a single syringe and that it can only take one drop.

So I got one of the syringes from one of the sponsors and used half of it and still had a half of syringe left. It still had large black clumps of spores in it so I figured that would be plenty to make three syringes out of.

So I took a wide mouth jelly jar and made an injection port and put 25cc of water in it. I then PC'd along with my wbs jars for 90 min.

I then cooled the jar and injection the half syringe into the jar through the injection port. I shook the jar to mix it well. Then I sucked up the spores into three full syringes.

I then inoculated 12quart jars of wbs. With 1/2cc per jar.

5 days in and I still see zero signs of growth. But when I originally used to original syringe I seen very noticeable growth in 4 days.


--------------------

Summer 09' Beautiful Plant (date on cam wrong)
**MiNdTwIsTeRs Trade List**


Extras: Filter Print Post Top
OfflineMachiavelliavore
Vermiculite Hater
 User Gallery


Registered: 12/08/14
Posts: 3,038
Loc: The Sporetorn States
Last seen: 3 months, 20 days
Re: Multiplying spore syringes? [Re: MiNdTwIsTeR]
    #22277338 - 09/23/15 02:29 AM (8 years, 4 months ago)

Well it's at 1/6 its original concentration, and what spores there were may not have been evenly distributed.  It may not be as quick.

In the future I would just do a quick invitro grow and make a sporeprint in the SAB.  Should be nice and clean.

I think you'll probably be sucessful though.


--------------------


I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister.  I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave.  I think it will be good cause B+ is so potent.
Triggered yet?

Only a square would say "a cube is a cube."


No, this does not look right...


Extras: Filter Print Post Top
OfflineLaughingcowwa
Your mum loves it.
Male


Registered: 05/02/15
Posts: 418
Last seen: 7 years, 9 months
Re: Multiplying spore syringes? [Re: Machiavelliavore]
    #22277345 - 09/23/15 02:34 AM (8 years, 4 months ago)

It's amazing how little amount of spores in a syringe works, I've seen some of the syringes you got off the sponsers and they are dark. You can hardly see the sprores when I make up a syringe and it always comes out.


--------------------
Those who doubt me, suck cock by choice


Extras: Filter Print Post Top
Offlinejcop
myself
Male User Gallery


Registered: 12/02/14
Posts: 163
Loc: Prague, CZ Flag
Last seen: 4 years, 3 months
Re: Multiplying spore syringes? [Re: Laughingcowwa]
    #22277508 - 09/23/15 05:44 AM (8 years, 4 months ago)

I find it better to use the rest of the syringe to make LC, LI or whatever...soo much quicker colonization.

You should be fine though, just give it a few more days :wink:


--------------------
Afraid of illness? German new medicine might be for you

First succesfull project:


Extras: Filter Print Post Top
InvisiblebodhisattaMDiscordReddit
Smurf real estate agent
 User Gallery
Folding@home Statistics
Registered: 04/30/13
Posts: 61,889
Loc: Milky way
Trusted Cultivator
Re: Multiplying spore syringes? [Re: jcop]
    #22277569 - 09/23/15 06:39 AM (8 years, 4 months ago)

Spores to LC is stupid. And you can't make LI with spores.


Extras: Filter Print Post Top
InvisibleMad Season
hookers and blackjack
 User Gallery


Registered: 09/16/12
Posts: 12,666
Loc: Canada Flag
Trusted Cultivator
Re: Multiplying spore syringes? [Re: bodhisatta]
    #22277597 - 09/23/15 07:00 AM (8 years, 4 months ago)

Yeah. In that case I'd just do agar.. you have to anyways for both LC and LI.

OP, I bet it'll be fine.


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


Extras: Filter Print Post Top
OfflinecronicrFacebook
 User Gallery


Registered: 08/07/11
Posts: 61,436
Loc: Van Isle Flag
Last seen: 2 years, 8 days
Trusted Cultivator
Re: Multiplying spore syringes? [Re: Mad Season]
    #22278149 - 09/23/15 10:27 AM (8 years, 4 months ago)

i always just suck water up in the syringe and refill but good luck!


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


Extras: Filter Print Post Top
Offlinejcop
myself
Male User Gallery


Registered: 12/02/14
Posts: 163
Loc: Prague, CZ Flag
Last seen: 4 years, 3 months
Re: Multiplying spore syringes? [Re: bodhisatta]
    #22278578 - 09/23/15 12:32 PM (8 years, 4 months ago)

Quote:

bodhisatta said:
Spores to LC is stupid. And you can't make LI with spores.




Could you elaborate? I had fine success with spores -> LC. And I meant spores -> grains -> LI

EDIT: I actually meant LGI, my bad there...


--------------------
Afraid of illness? German new medicine might be for you

First succesfull project:


Edited by jcop (09/23/15 12:34 PM)


Extras: Filter Print Post Top
InvisibleMad Season
hookers and blackjack
 User Gallery


Registered: 09/16/12
Posts: 12,666
Loc: Canada Flag
Trusted Cultivator
Re: Multiplying spore syringes? [Re: jcop]
    #22278632 - 09/23/15 12:42 PM (8 years, 4 months ago)

You had fine success. This is from a syringe i had the luck of getting.



This particular syringe in lc would have been shit. Luckily I used agar, and was able to salvage it. As seen by me cutting it.

Agar is just as easy to make as LC, and it's much safer. If it's clean spores, great! :thumbup: but I like getting success every time.


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


Extras: Filter Print Post Top
Offlinejcop
myself
Male User Gallery


Registered: 12/02/14
Posts: 163
Loc: Prague, CZ Flag
Last seen: 4 years, 3 months
Re: Multiplying spore syringes? [Re: Mad Season]
    #22278763 - 09/23/15 01:05 PM (8 years, 4 months ago)

Get it, thanks :thumbup:

Is it usual to get contamined syringes from sponsors though?


--------------------
Afraid of illness? German new medicine might be for you

First succesfull project:


Extras: Filter Print Post Top
InvisibleMad Season
hookers and blackjack
 User Gallery


Registered: 09/16/12
Posts: 12,666
Loc: Canada Flag
Trusted Cultivator
Re: Multiplying spore syringes? [Re: jcop]
    #22278800 - 09/23/15 01:14 PM (8 years, 4 months ago)

I'd say around half my syringes have had some sort of contamination in them.


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.


Extras: Filter Print Post Top
InvisiblebodhisattaMDiscordReddit
Smurf real estate agent
 User Gallery
Folding@home Statistics
Registered: 04/30/13
Posts: 61,889
Loc: Milky way
Trusted Cultivator
Re: Multiplying spore syringes? [Re: Mad Season]
    #22279125 - 09/23/15 02:32 PM (8 years, 4 months ago)

Every syringe has at least one bacterium in it unless you grow in-vitro and print in a clean room.

Most spore syringes maybe have something like 1 bacteria per 1,000,000+ cube spores. This widely varries on how well it was made.

If you shoot 1cc into a cake no big deal you'll probably be fine even if one bacterium gets in. Same on a petri dish you can just isolate away from the little bacteria colony.

With a LC even one bacterium will just keep growing and spoil the whole thing. You may get lucky. But spores to LC is just plain fucking stupid to most of us


Extras: Filter Print Post Top
Offlinejcop
myself
Male User Gallery


Registered: 12/02/14
Posts: 163
Loc: Prague, CZ Flag
Last seen: 4 years, 3 months
Re: Multiplying spore syringes? [Re: bodhisatta]
    #22279154 - 09/23/15 02:40 PM (8 years, 4 months ago)

Quote:

bodhisatta said:
Every syringe has at least one bacterium in it unless you grow in-vitro and print in a clean room.

Most spore syringes maybe have something like 1 bacteria per 1,000,000+ cube spores. This widely varries on how well it was made.

If you shoot 1cc into a cake no big deal you'll probably be fine even if one bacterium gets in. Same on a petri dish you can just isolate away from the little bacteria colony.

With a LC even one bacterium will just keep growing and spoil the whole thing. You may get lucky. But spores to LC is just plain fucking stupid to most of us




I'm glad I got so lucky then :shrug:, will not repeat :thumbup:
GLI sounds reasonable then, right?


--------------------
Afraid of illness? German new medicine might be for you

First succesfull project:


Extras: Filter Print Post Top
Invisiblenatedawgnow
Rocky mountain hood rat
 User Gallery


Registered: 02/09/15
Posts: 8,939
Loc: ation
Trusted Cultivator
Re: Multiplying spore syringes? [Re: jcop]
    #22279243 - 09/23/15 03:08 PM (8 years, 4 months ago)

Only if your initial spawn is clean as a fuckin whistle.

It is still pretty risky since a lot of people have trouble making
perfectly clean spawn, including myself on occasion.


--------------------


Extras: Filter Print Post Top
InvisiblebodhisattaMDiscordReddit
Smurf real estate agent
 User Gallery
Folding@home Statistics
Registered: 04/30/13
Posts: 61,889
Loc: Milky way
Trusted Cultivator
Re: Multiplying spore syringes? [Re: natedawgnow]
    #22279353 - 09/23/15 03:34 PM (8 years, 4 months ago)

I've never been a fan. if you want to do a LC use an agar wedge into grain soak water or make any agar recipe for fungi and omit the agar-agar(they call this broth instead of agar)


Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Mushroom-Hut Liquid Cultures   Original Sensible Seeds Bulk Cannabis Seeds   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Left Coast Kratom Buy Kratom Extract   North Spore Injection Grain Bag


Similar ThreadsPosterViewsRepliesLast post
* spore syring is in trouble? mulan 14,123 12 08/29/09 09:15 PM
by HaRo On KoRn
* Spore Syring Cont/Clear Misconcept if any Zwieback0 5,674 4 08/11/08 03:22 AM
by fr33d0mfry
* Re: When buying a spore syring... 3Mshroom 7,932 2 06/01/00 08:17 PM
by 3Mshroom
* Spore Bacterial Infection?
( 1 2 all )
BladeLSD 12,139 34 05/21/03 03:30 PM
by Suntzu
* Re: Spore transfer? Anonymous 1,901 1 03/08/01 08:17 PM
by Juggles
* how far to spore prints go? AKA PC 3,737 11 01/28/03 08:07 AM
by debianlinux
* Possible Contaminated Spore Syringe? Tim2133 2,916 5 06/30/03 11:09 PM
by Sev
* spores userdoesnotexist 1,508 5 08/06/02 07:52 AM
by mickey_rourke

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
2,758 topic views. 33 members, 346 guests and 82 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.03 seconds spending 0.007 seconds on 12 queries.