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MiNdTwIsTeR
ThirdEye

Registered: 10/14/08
Posts: 427
Last seen: 7 years, 3 hours
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Multiplying spore syringes?
#22277282 - 09/23/15 01:40 AM (8 years, 4 months ago) |
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I've read over and over that there's an over abundance of spores in a single syringe and that it can only take one drop.
So I got one of the syringes from one of the sponsors and used half of it and still had a half of syringe left. It still had large black clumps of spores in it so I figured that would be plenty to make three syringes out of.
So I took a wide mouth jelly jar and made an injection port and put 25cc of water in it. I then PC'd along with my wbs jars for 90 min.
I then cooled the jar and injection the half syringe into the jar through the injection port. I shook the jar to mix it well. Then I sucked up the spores into three full syringes.
I then inoculated 12quart jars of wbs. With 1/2cc per jar.
5 days in and I still see zero signs of growth. But when I originally used to original syringe I seen very noticeable growth in 4 days.
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Summer 09' Beautiful Plant (date on cam wrong) **MiNdTwIsTeRs Trade List**
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Machiavelliavore
Vermiculite Hater



Registered: 12/08/14
Posts: 3,038
Loc: The Sporetorn States
Last seen: 3 months, 20 days
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Re: Multiplying spore syringes? [Re: MiNdTwIsTeR]
#22277338 - 09/23/15 02:29 AM (8 years, 4 months ago) |
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Well it's at 1/6 its original concentration, and what spores there were may not have been evenly distributed. It may not be as quick.
In the future I would just do a quick invitro grow and make a sporeprint in the SAB. Should be nice and clean.
I think you'll probably be sucessful though.
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I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister. I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave. I think it will be good cause B+ is so potent. Triggered yet? Only a square would say "a cube is a cube."
No, this does not look right...
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Laughingcowwa
Your mum loves it.



Registered: 05/02/15
Posts: 418
Last seen: 7 years, 9 months
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It's amazing how little amount of spores in a syringe works, I've seen some of the syringes you got off the sponsers and they are dark. You can hardly see the sprores when I make up a syringe and it always comes out.
-------------------- Those who doubt me, suck cock by choice
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jcop
myself



Registered: 12/02/14
Posts: 163
Loc: Prague, CZ
Last seen: 4 years, 3 months
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I find it better to use the rest of the syringe to make LC, LI or whatever...soo much quicker colonization.
You should be fine though, just give it a few more days
-------------------- Afraid of illness? German new medicine might be for you First succesfull project:
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Multiplying spore syringes? [Re: jcop]
#22277569 - 09/23/15 06:39 AM (8 years, 4 months ago) |
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Spores to LC is stupid. And you can't make LI with spores.
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Re: Multiplying spore syringes? [Re: bodhisatta]
#22277597 - 09/23/15 07:00 AM (8 years, 4 months ago) |
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Yeah. In that case I'd just do agar.. you have to anyways for both LC and LI.
OP, I bet it'll be fine.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: Multiplying spore syringes? [Re: Mad Season]
#22278149 - 09/23/15 10:27 AM (8 years, 4 months ago) |
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i always just suck water up in the syringe and refill but good luck!
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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jcop
myself



Registered: 12/02/14
Posts: 163
Loc: Prague, CZ
Last seen: 4 years, 3 months
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Re: Multiplying spore syringes? [Re: bodhisatta]
#22278578 - 09/23/15 12:32 PM (8 years, 4 months ago) |
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Quote:
bodhisatta said: Spores to LC is stupid. And you can't make LI with spores.
Could you elaborate? I had fine success with spores -> LC. And I meant spores -> grains -> LI
EDIT: I actually meant LGI, my bad there...
-------------------- Afraid of illness? German new medicine might be for you First succesfull project:
Edited by jcop (09/23/15 12:34 PM)
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Re: Multiplying spore syringes? [Re: jcop]
#22278632 - 09/23/15 12:42 PM (8 years, 4 months ago) |
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You had fine success. This is from a syringe i had the luck of getting.

This particular syringe in lc would have been shit. Luckily I used agar, and was able to salvage it. As seen by me cutting it.
Agar is just as easy to make as LC, and it's much safer. If it's clean spores, great! but I like getting success every time.
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jcop
myself



Registered: 12/02/14
Posts: 163
Loc: Prague, CZ
Last seen: 4 years, 3 months
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Re: Multiplying spore syringes? [Re: Mad Season]
#22278763 - 09/23/15 01:05 PM (8 years, 4 months ago) |
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Get it, thanks 
Is it usual to get contamined syringes from sponsors though?
-------------------- Afraid of illness? German new medicine might be for you First succesfull project:
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Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
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Re: Multiplying spore syringes? [Re: jcop]
#22278800 - 09/23/15 01:14 PM (8 years, 4 months ago) |
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I'd say around half my syringes have had some sort of contamination in them.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Multiplying spore syringes? [Re: Mad Season]
#22279125 - 09/23/15 02:32 PM (8 years, 4 months ago) |
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Every syringe has at least one bacterium in it unless you grow in-vitro and print in a clean room.
Most spore syringes maybe have something like 1 bacteria per 1,000,000+ cube spores. This widely varries on how well it was made.
If you shoot 1cc into a cake no big deal you'll probably be fine even if one bacterium gets in. Same on a petri dish you can just isolate away from the little bacteria colony.
With a LC even one bacterium will just keep growing and spoil the whole thing. You may get lucky. But spores to LC is just plain fucking stupid to most of us
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jcop
myself



Registered: 12/02/14
Posts: 163
Loc: Prague, CZ
Last seen: 4 years, 3 months
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Re: Multiplying spore syringes? [Re: bodhisatta]
#22279154 - 09/23/15 02:40 PM (8 years, 4 months ago) |
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Quote:
bodhisatta said: Every syringe has at least one bacterium in it unless you grow in-vitro and print in a clean room.
Most spore syringes maybe have something like 1 bacteria per 1,000,000+ cube spores. This widely varries on how well it was made.
If you shoot 1cc into a cake no big deal you'll probably be fine even if one bacterium gets in. Same on a petri dish you can just isolate away from the little bacteria colony.
With a LC even one bacterium will just keep growing and spoil the whole thing. You may get lucky. But spores to LC is just plain fucking stupid to most of us
I'm glad I got so lucky then , will not repeat  GLI sounds reasonable then, right?
-------------------- Afraid of illness? German new medicine might be for you First succesfull project:
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natedawgnow
Rocky mountain hood rat



Registered: 02/09/15
Posts: 8,939
Loc: ation
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Re: Multiplying spore syringes? [Re: jcop]
#22279243 - 09/23/15 03:08 PM (8 years, 4 months ago) |
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Only if your initial spawn is clean as a fuckin whistle.
It is still pretty risky since a lot of people have trouble making perfectly clean spawn, including myself on occasion.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Multiplying spore syringes? [Re: natedawgnow]
#22279353 - 09/23/15 03:34 PM (8 years, 4 months ago) |
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I've never been a fan. if you want to do a LC use an agar wedge into grain soak water or make any agar recipe for fungi and omit the agar-agar(they call this broth instead of agar)
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