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Sivarted
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Registered: 08/22/15
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Wbs not-exactly-ideal inoculation
#22275063 - 09/22/15 04:45 PM (8 years, 4 months ago) |
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So, I did something that I now recognize as slightly not ideal, by inoculating pint jars of wbs with a spore syringe, putting spore solution in the middle of the tops of the jar.
I'm in the middle of a successful BRF jar run, and wanted to play around with wbs both for GLC and as spawn for a future monotub attempt, so I figured I might as well familiarize myself with the process.
But since it's my first time, I really wish I would have inoculated the sides of the jar so I could see growth asap, and determine whether that first attempt was actually successful sone rather than later.
But since I did what I did, does anybody have experience inoculating the center of the jar and know how soon growth might show up, and where and how?
I'm sort of expecting that if my procedure was in fact successful, that I won't see anything for a long time until it explodes out of the center of the jar, but I don't actually know what to expect.
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Machiavelliavore
Vermiculite Hater



Registered: 12/08/14
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Re: Wbs not-exactly-ideal inoculation [Re: Sivarted]
#22275081 - 09/22/15 04:48 PM (8 years, 4 months ago) |
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Yeah, bottom is probably your best chance to see anything.
Given your PF run, you probably have a rough idea of your spore germination time.
Add like 5 days to that, and then give it a gentle shake and see if there's a large clump in the middle. If so, shake'r hard to distribute and inoculate the whole jar.
No reason it shouldn't work.
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I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister. I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave. I think it will be good cause B+ is so potent. Triggered yet? Only a square would say "a cube is a cube."
No, this does not look right...
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Sivarted
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Registered: 08/22/15
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Well, the syringe I used wasn't the same one I used from the initial run, it was a syringe I made myself from a spore print I took from my very first mushroom that happened to be a gigantor that started invitro.
Fortunately I do know the syringe itself was viable because in addition to 4 pints of wbs, I knocked up 13 brf jars (that in combination with using my own spore print, I made myself from start to finish to prove self-sufficiency), and those are all growing quite nicely and so far contam-free, so I'm hopeful about the wbs too. 
Just wish I had inoculated the sides, because I know the spores are viable but I don't know if I got the moisture content right on the wbs. Lol.
Thinking what I might do is give them another 4-5 days and if there's not visible growth, tear one of the jars apart to see what they look like inside. I did 4 of them in hopes of being able to do at least one GLC jar, and I have plenty more seed (and 5 good sized spore prints), so it wouldn't be the end of the world to lose one.
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Machiavelliavore
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Re: Wbs not-exactly-ideal inoculation [Re: Sivarted]
#22275218 - 09/22/15 05:11 PM (8 years, 4 months ago) |
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Well, from a fresh print of a non-mutant species, you can probably expect it to germinate in like 7 days. I'd just give them a good hard shake after 14 days. There should be clumps of mycellium in the middle. No need to waste what might be a good jar. Schrodinger quarts, yo.
I wouldn't try the GLC thing, especially from a print incoulated jar. They're usually just a little bit dirty, but the mushroom mycellium wins out statistically. G2G, and you maintain or improve on that statistical advantage. Suck up some random water, and you don't know what you might be getting statistically, and you're spreading it all over.
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I spawned some popcorn casings and had double-overlay cause I didn't put enough hydrogen peroxide in my automated aquarium mister. I only got one mushroom so I cut off the head part where the seeds fall from and put it in a jar of LC and sprayed it all over a tin of PF cakes I made with gravel, cardboard, and bisquick in my microwave. I think it will be good cause B+ is so potent. Triggered yet? Only a square would say "a cube is a cube."
No, this does not look right...
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Bugler Boy
Cultivar


Registered: 09/19/11
Posts: 998
Loc: In your head
Last seen: 2 years, 8 months
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Even if you went straight in the center gravity should bring some of the solution/spores to the bottom. Look for growth on the very bottom
-------------------- The mushroom speaks: '"I am old, older than thought in your species, which is itself fifty times older than your history. Though I have been on earth for ages I am from the stars. My home is no one planet, for many worlds scattered through the shining disc of the galaxy have conditions which allow my spores an opportunity for life... How the hypercommunication mode operates is a secret which will not be lightly given to man. But the means should be obvious: it is the occurrence of psilocybin and psilocin in the biosynthetic pathways of my living body that opens for me and my symbiots the vision screens to many worlds"
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Sivarted
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Re: Wbs not-exactly-ideal inoculation [Re: Bugler Boy]
#22328520 - 10/03/15 02:09 PM (8 years, 3 months ago) |
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Two of the jars ended up showing myc on the top, still haven't seen anything on the bottom of any of them.
Few more days and I'll probably go ahead and give them a shake.
I have another 7 jars of wbs that I did a couple days later, inoculated at two points down the sides, and those are all showing growth, so I'll probably use those as a guide for when to shake the first couple.
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