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OfflineMajora123
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Dried shroom cloning agar. Growth need help!
    #22273550 - 09/22/15 11:08 AM (8 years, 4 months ago)

Hello! I need help! I live I've CA and Connot order shrooms. I had cut a put of inside tissue from a dried shroom and also put gills on top of two other plates. I did this in a still air box. It's been a week and this is what is growing on them. Is this healthy mycelium or should I transfer them? I have bought everything I needed to start cakes can anyone let me know there opinions please help!


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Offlinemidnightmaraude
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Re: Dried shroom cloning agar. Growth need help! [Re: Majora123]
    #22273556 - 09/22/15 11:09 AM (8 years, 4 months ago)

interesting.


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InvisibleInocuole
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Re: Dried shroom cloning agar. Growth need help! [Re: midnightmaraude]
    #22273572 - 09/22/15 11:15 AM (8 years, 4 months ago)

First two pics, kinda fluffy but there's some sectoring, seems like your best shot.  Transfer away from some of the edges there or wait for it to see if it goes rhizomorphic before transferring.


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OfflineMajora123
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Re: Dried shroom cloning agar. Growth need help! [Re: Inocuole]
    #22273673 - 09/22/15 11:53 AM (8 years, 4 months ago)

Quote:

Inocuole said:
First two pics, kinda fluffy but there's some sectoring, seems like your best shot.  Transfer away from some of the edges there or wait for it to see if it goes rhizomorphic before transferring.



This was my first time doing any of this and was lucky their is no really nasty stuff in there. The first night I did not know that I had to keep the plate upside down so in the morning there was pools of water in all of them I thought that I messed up. I quickly turned turn upside down. The first two pics from a dried tissue sample looks kind of gooey. I Think that one is contaiminated it doesn't look like the others. Should I just wait and how long does it normally take for a good sample? Thanks!


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OfflineMajora123
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Re: Dried shroom cloning agar. Growth need help! [Re: Majora123]
    #22273696 - 09/22/15 11:57 AM (8 years, 4 months ago)

Here's a picture in the light of pic 1 & 2


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OfflineYaMoonSun
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Re: Dried shroom cloning agar. Growth need help! [Re: Majora123]
    #22273744 - 09/22/15 12:09 PM (8 years, 4 months ago)

That's hilarious that you jumped right into agar, but congrats, that looks like healthy myc to me. Maybe transfer a sector to grain? You working with a still-air-box?


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Offlinemidnightmaraude
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Re: Dried shroom cloning agar. Growth need help! [Re: YaMoonSun]
    #22273803 - 09/22/15 12:23 PM (8 years, 4 months ago)

I'm pretty impressed there are no visible contams using dried shroom that has been in contact with everything probably.  Good start.


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OfflineMajora123
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Re: Dried shroom cloning agar. Growth need help! [Re: YaMoonSun]
    #22273823 - 09/22/15 12:28 PM (8 years, 4 months ago)

Quote:

YaMoonSun said:
That's hilarious that you jumped right into agar, but congrats, that looks like healthy myc to me. Maybe transfer a sector to grain? You working with a still-air-box?


I dont think its heathy. It's a little yellower than the others and more moist. Here are better pics.



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OfflineMajora123
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Re: Dried shroom cloning agar. Growth need help! [Re: midnightmaraude]
    #22273842 - 09/22/15 12:32 PM (8 years, 4 months ago)

Quote:

midnightmaraude said:
I'm pretty impressed there are no visible contams using dried shroom that has been in contact with everything probably.  Good start.



Thank you! Is this looking pretty normal or should I transfer the edges to a newer plates? I used pieces from the inside of the tissue and gills that were still under some unbroken veils. Nature always finds a way. There are many people that said I will get get any king of growth other than mold. I was going to quit but wanted to experiment. It is hard for me to get shrooms here everybody always just bullshits so I wanted my own harvest.


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OfflineYaMoonSun
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Re: Dried shroom cloning agar. Growth need help! [Re: Majora123]
    #22273862 - 09/22/15 12:36 PM (8 years, 4 months ago)

I think I heard of some anti-bacterial agar, try to find some of that, and when you take a transfer it should clean it up. You nor-cal?


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OfflineMajora123
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Re: Dried shroom cloning agar. Growth need help! [Re: YaMoonSun]
    #22273919 - 09/22/15 12:48 PM (8 years, 4 months ago)

Quote:

YaMoonSun said:
I think I heard of some anti-bacterial agar, try to find some of that, and when you take a transfer it should clean it up. You nor-cal?



I bought these plates from Amazon. They said they were sterile. I'm in the LA area. So I shouldn'tuse any of these without transferring? Should I transfer all of them to new plates just invade? Or do you think I can use any of these to cut up and put in grain jars and start already with growing?


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InvisibleInocuole
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Re: Dried shroom cloning agar. Growth need help! [Re: Majora123]
    #22274023 - 09/22/15 01:12 PM (8 years, 4 months ago)

Well if it's yellow I don't know, I don't personally like the look of any of that growth.  Maybe wait a little longer or see if you can get any more healthy looking growth..  Or keep transferring from what you have and see what that yields.


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OfflineYaMoonSun
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Re: Dried shroom cloning agar. Growth need help! [Re: Majora123]
    #22274059 - 09/22/15 01:18 PM (8 years, 4 months ago)

All usable agar has to be sterilized beforehand, but not all of it contains anti-bacterials.


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OfflineMajora123
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Re: Dried shroom cloning agar. Growth need help! [Re: Inocuole]
    #22274084 - 09/22/15 01:22 PM (8 years, 4 months ago)

Quote:

Inocuole said:
Well if it's yellow I don't know, I don't personally like the look of any of that growth.  Maybe wait a little longer or see if you can get any more healthy looking growth..  Or keep transferring from what you have and see what that yields.



Not even the ones on top? How are the more whiter transpare ones?


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InvisibleJuiceh
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Re: Dried shroom cloning agar. Growth need help! [Re: Majora123]
    #22274173 - 09/22/15 01:43 PM (8 years, 4 months ago)

Looks like nothing but bacteria to me in the last pics.

Some pics in your first post looked more like mycelium. But the last batch of pics. It all looks like melted yellowish ice cream. No Bueno.


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Edited by Juiceh (09/22/15 01:45 PM)


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Invisibletahoe
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Re: Dried shroom cloning agar. Growth need help! [Re: Juiceh]
    #22274367 - 09/22/15 02:26 PM (8 years, 4 months ago)

I agree. Looks milky and like bacteria. The texture is all wrong. Plus, growing from dried material to agar takes longer than a few days. Usually two weeks or more to get the mycelium up and growing.

Majora. Do you have a pressure cooker? You are only going to be able to get away with mail order plates for a short time. There should only be growth where you have actually put material on the agar.
You will have better luck using spore/gill fragment.
Get a decent pic of your plates. They should kind of look like this. Don't think that your plates might dry up and look like this. There should be visible structure to the mycelium. Not gooey or slimy.


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Re: Dried shroom cloning agar. Growth need help! [Re: tahoe]
    #22274387 - 09/22/15 02:29 PM (8 years, 4 months ago)

Majora, some of the upper ones look okay. But the plates with multiple spots on them are bad. Unless you purposely placed material on multiple spots.


--------------------
Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


My Legacy
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InvisibleJuiceh
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Re: Dried shroom cloning agar. Growth need help! [Re: tahoe]
    #22274661 - 09/22/15 03:29 PM (8 years, 4 months ago)

Quote:

tahoe said:
Majora, some of the upper ones look okay. But the plates with multiple spots on them are bad. Unless you purposely placed material on multiple spots.



:whathesaid:

You do not want anything growing elsewhere from where you put the tissue sample.


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OfflineMajora123
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Re: Dried shroom cloning agar. Growth need help! [Re: tahoe]
    #22275054 - 09/22/15 04:43 PM (8 years, 4 months ago)

Quote:

tahoe said:
I agree. Looks milky and like bacteria. The texture is all wrong. Plus, growing from dried material to agar takes longer than a few days. Usually two weeks or more to get the mycelium up and growing.

Majora. Do you have a pressure cooker? You are only going to be able to get away with mail order plates for a short time. There should only be growth where you have actually put material on the agar.
You will have better luck using spore/gill fragment.
Get a decent pic of your plates. They should kind of look like this. Don't think that your plates might dry up and look like this. There should be visible structure to the mycelium. Not gooey or slimy.




The first night I had put them on, I didn't know I was supposed to keep them upside down. I'm guessing some of the tissue spread around so that's why there's growth all around. So none of them look like mycelium? It's been a week a day since I put them on. Is that to fast?


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OfflineMajora123
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Re: Dried shroom cloning agar. Growth need help! [Re: tahoe]
    #22275064 - 09/22/15 04:45 PM (8 years, 4 months ago)

Quote:

tahoe said:
Majora, some of the upper ones look okay. But the plates with multiple spots on them are bad. Unless you purposely placed material on multiple spots.



The first night I did not know to store them upside down. When I woke up in the morning there was a pool of water. I'm thinking that it may had spread.


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