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Offlineoysterlover
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Registered: 04/19/11
Posts: 166
Last seen: 5 years, 5 months
First Tampanensis grow - stones and fruits
    #22273390 - 09/22/15 10:22 AM (8 years, 4 months ago)

So I received a TC of p. tampanensis back in May.  This would be the first time I'd ever tried cultivating stone producers, and I have to say I am hooked!

I started with agar.  Did a little isolation and grew out a few plates of myc from my original wedge.  The myc seems to cultivate just as easily on agar as any other cube I've worked with.  After the first batch, I started using agar made with the water left over after simmering my grass seed.  I read about this somewhere on here....

Some of the myc even started forming "roots" and stones on agar.  Pretty interesting to see.  Another note:  All of my plates eventually pinned in-vitro.  Guess that's just something tampanensis likes to do.



After agar, I transferred some wedges to prepped RGS using Violet's method.  I don't remember where I found it, but you can search the forums for it.  Basically the RGS is simmered and PCed.  No muss, no fuss.  The RGS jars colonized quickly and evenly.  I did a few quarts and a few pints.  I also inoculated some mexicana A along with the tampenensis, just to do a comparison.
Sclerotia started to form visibly after only a few weeks on the insides of the jars of tampanensis, but not at all with the Mex A.  I read somewhere that this is a characteristic of the strains and not to be concerned.


tampanensis sclerotia


mex-A after about 2 months colonization.


After about 6 weeks I harvested one of the pints of each strain.  I believe I got about 20g wet from one jar of tampanensis.  Not a huge harvest, but not bad either.  The Mex A jar yielded ZERO stones.  no idea what happened, but I assumed I just harvested too early.  I currently have several more jars going, my longest being one inoculated on  6/12.  We'll see what those produce.  One thing to note, these jars are totally neglected.  They don't even have GE holes in the lids.  All I have done since inoculation is put them in a drawer and look at them every so often.  No shaking.  Set it and forget it!





  After harvesting the sclerotia, I was left with a large pile of colonized loose grass seed.  I put it all back in the jars it came out of.  I noticed it "fluffed up" like cube myc, but never really consolidated back together.  I figured I'd try to case it and see what happened.

  I decided to case in quart sized deli containers, simply because that was what I had on hand.  I layered the RGS with coffee soaked and pasteurized coir, alternating in about 1" thick layers.  I popped the lid on the pint container and let it colonize.  Admittedly, I forgot about it for about a month.
  After a month, the myc was just starting to appear on the top of the casing, so into the fruiting chamber it went.  For a couple weeks it just sat there, not really doing anything.  I didn't pay much attention, as I had other stuff going on.  When I finally checked it, I realized the entire casing had completely dried out.  In an effort to save it, I filled the quart container with water, and put the lid back on overnight.  In the morning, I dumped out the remaining water and put it back in the fruiting chamber.  Within a couple days, I saw this:



  After that, things really started going crazy.  This small casing has produced about a dozen fruits, all over 6" tall.  I have made a few prints, which is very exciting.  I've also noticed several HUGE stones forming.  A couple of these are right at the surface of the casing, and have grown so large they have broken through the surface.  I will try to get pictures of the casing tonight and add them. 

  After reading more on fruiting tampanensis, I believe this dry period is the key to fruiting them.  I've only done it once, but it seems to mimic the conditions that would trigger fruiting in the wild.  I plan to experiment further with that theory.

  Just wanted to say that I am totally in love with tampanensis.  I really like the "set it and forget it" cultivation method, and the fact that I can get stones AND fruits.


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Contaminate is a verb.  Contaminant is a noun.
:amanita2::amanita2::amanita2:Trade List:amanita2::amanita2::amanita2:


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Invisiblesweetpea
Female
Registered: 12/29/13
Posts: 327
. [Re: oysterlover]
    #22273474 - 09/22/15 10:45 AM (8 years, 4 months ago)

.


Edited by sweetpea (10/06/15 12:44 PM)


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OfflineP.Zappatecorum
Lophophilus
Male


Registered: 10/15/12
Posts: 2,094
Loc: Cactaceae
Last seen: 3 years, 5 months
Re: First Tampanensis grow - stones and fruits [Re: oysterlover]
    #22273478 - 09/22/15 10:46 AM (8 years, 4 months ago)

Yeah dude, I got my best stones during the fruiting process. 
:asianofapproval:


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Offlineoysterlover
Stranger

Registered: 04/19/11
Posts: 166
Last seen: 5 years, 5 months
Re: First Tampanensis grow - stones and fruits [Re: P.Zappatecorum] * 1
    #22273565 - 09/22/15 11:13 AM (8 years, 4 months ago)

More pics to come...I'll take some tonight


--------------------
Contaminate is a verb.  Contaminant is a noun.
:amanita2::amanita2::amanita2:Trade List:amanita2::amanita2::amanita2:


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Offlineoysterlover
Stranger

Registered: 04/19/11
Posts: 166
Last seen: 5 years, 5 months
Re: First Tampanensis grow - stones and fruits [Re: oysterlover]
    #22274932 - 09/22/15 04:18 PM (8 years, 4 months ago)

Here are the fruits that are left after harvesting about 8 for prints

[/url


--------------------
Contaminate is a verb.  Contaminant is a noun.
:amanita2::amanita2::amanita2:Trade List:amanita2::amanita2::amanita2:


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OfflinecronicrFacebook
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Registered: 08/07/11
Posts: 61,436
Loc: Van Isle Flag
Last seen: 2 years, 8 days
Trusted Cultivator
Re: First Tampanensis grow - stones and fruits [Re: oysterlover]
    #22274937 - 09/22/15 04:18 PM (8 years, 4 months ago)

:cool: great stuff


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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OfflineSgt. Overkill
Agar Warrior


Registered: 05/24/16
Posts: 10
Last seen: 6 years, 4 months
Re: First Tampanensis grow - stones and fruits [Re: oysterlover]
    #23620331 - 09/06/16 11:20 PM (7 years, 4 months ago)

From what i know, Tampanensis requires constant fresh air and is a better performer outdoors mainly for that reason. If you can find a way to constantly supply fresh humid air to your fruiting chamber you'd probably get decent sized mushies and a way better yield! Other successful growers have also had their casing and spawn dehydrated by accident and then rehydrated, and saw good pinning (clusters) in a matter of 2 weeks, so that's probably not a coincidence! :smile:


--------------------
Sgt. Overkill of the 23rd Agar Division, Reporting for Duty!


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