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oysterlover
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Registered: 04/19/11
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First Tampanensis grow - stones and fruits
#22273390 - 09/22/15 10:22 AM (8 years, 4 months ago) |
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So I received a TC of p. tampanensis back in May. This would be the first time I'd ever tried cultivating stone producers, and I have to say I am hooked!
I started with agar. Did a little isolation and grew out a few plates of myc from my original wedge. The myc seems to cultivate just as easily on agar as any other cube I've worked with. After the first batch, I started using agar made with the water left over after simmering my grass seed. I read about this somewhere on here....
Some of the myc even started forming "roots" and stones on agar. Pretty interesting to see. Another note: All of my plates eventually pinned in-vitro. Guess that's just something tampanensis likes to do.

After agar, I transferred some wedges to prepped RGS using Violet's method. I don't remember where I found it, but you can search the forums for it. Basically the RGS is simmered and PCed. No muss, no fuss. The RGS jars colonized quickly and evenly. I did a few quarts and a few pints. I also inoculated some mexicana A along with the tampenensis, just to do a comparison. Sclerotia started to form visibly after only a few weeks on the insides of the jars of tampanensis, but not at all with the Mex A. I read somewhere that this is a characteristic of the strains and not to be concerned.
 tampanensis sclerotia
 mex-A after about 2 months colonization.
After about 6 weeks I harvested one of the pints of each strain. I believe I got about 20g wet from one jar of tampanensis. Not a huge harvest, but not bad either. The Mex A jar yielded ZERO stones. no idea what happened, but I assumed I just harvested too early. I currently have several more jars going, my longest being one inoculated on 6/12. We'll see what those produce. One thing to note, these jars are totally neglected. They don't even have GE holes in the lids. All I have done since inoculation is put them in a drawer and look at them every so often. No shaking. Set it and forget it!


After harvesting the sclerotia, I was left with a large pile of colonized loose grass seed. I put it all back in the jars it came out of. I noticed it "fluffed up" like cube myc, but never really consolidated back together. I figured I'd try to case it and see what happened.
I decided to case in quart sized deli containers, simply because that was what I had on hand. I layered the RGS with coffee soaked and pasteurized coir, alternating in about 1" thick layers. I popped the lid on the pint container and let it colonize. Admittedly, I forgot about it for about a month. After a month, the myc was just starting to appear on the top of the casing, so into the fruiting chamber it went. For a couple weeks it just sat there, not really doing anything. I didn't pay much attention, as I had other stuff going on. When I finally checked it, I realized the entire casing had completely dried out. In an effort to save it, I filled the quart container with water, and put the lid back on overnight. In the morning, I dumped out the remaining water and put it back in the fruiting chamber. Within a couple days, I saw this:

After that, things really started going crazy. This small casing has produced about a dozen fruits, all over 6" tall. I have made a few prints, which is very exciting. I've also noticed several HUGE stones forming. A couple of these are right at the surface of the casing, and have grown so large they have broken through the surface. I will try to get pictures of the casing tonight and add them.
After reading more on fruiting tampanensis, I believe this dry period is the key to fruiting them. I've only done it once, but it seems to mimic the conditions that would trigger fruiting in the wild. I plan to experiment further with that theory.
Just wanted to say that I am totally in love with tampanensis. I really like the "set it and forget it" cultivation method, and the fact that I can get stones AND fruits.
-------------------- Contaminate is a verb. Contaminant is a noun.
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sweetpea

Registered: 12/29/13
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Edited by sweetpea (10/06/15 12:44 PM)
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P.Zappatecorum
Lophophilus



Registered: 10/15/12
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Re: First Tampanensis grow - stones and fruits [Re: oysterlover]
#22273478 - 09/22/15 10:46 AM (8 years, 4 months ago) |
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Yeah dude, I got my best stones during the fruiting process.
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oysterlover
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Registered: 04/19/11
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Re: First Tampanensis grow - stones and fruits [Re: P.Zappatecorum] 1
#22273565 - 09/22/15 11:13 AM (8 years, 4 months ago) |
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More pics to come...I'll take some tonight
-------------------- Contaminate is a verb. Contaminant is a noun.
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oysterlover
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Re: First Tampanensis grow - stones and fruits [Re: oysterlover]
#22274932 - 09/22/15 04:18 PM (8 years, 4 months ago) |
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Here are the fruits that are left after harvesting about 8 for prints
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cronicr



Registered: 08/07/11
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Re: First Tampanensis grow - stones and fruits [Re: oysterlover]
#22274937 - 09/22/15 04:18 PM (8 years, 4 months ago) |
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great stuff
--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Sgt. Overkill
Agar Warrior


Registered: 05/24/16
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Re: First Tampanensis grow - stones and fruits [Re: oysterlover]
#23620331 - 09/06/16 11:20 PM (7 years, 4 months ago) |
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From what i know, Tampanensis requires constant fresh air and is a better performer outdoors mainly for that reason. If you can find a way to constantly supply fresh humid air to your fruiting chamber you'd probably get decent sized mushies and a way better yield! Other successful growers have also had their casing and spawn dehydrated by accident and then rehydrated, and saw good pinning (clusters) in a matter of 2 weeks, so that's probably not a coincidence!
-------------------- Sgt. Overkill of the 23rd Agar Division, Reporting for Duty!
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